Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla
A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from Pilea microphylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/826830 |
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| author | Amir Modarresi Chahardehi Darah Ibrahim Shaida Fariza Sulaiman |
| author_facet | Amir Modarresi Chahardehi Darah Ibrahim Shaida Fariza Sulaiman |
| author_sort | Amir Modarresi Chahardehi |
| collection | DOAJ |
| description | A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from Pilea microphylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at 72.10±0.71 and chloroform extract of method II (CE II) showed the highest total flavonoid contents at 60.14±0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry. |
| format | Article |
| id | doaj-art-49801871feba4221972f671fcbdaf2e2 |
| institution | DOAJ |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-49801871feba4221972f671fcbdaf2e22025-08-20T02:39:21ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/826830826830Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphyllaAmir Modarresi Chahardehi0Darah Ibrahim1Shaida Fariza Sulaiman2Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800 Minden, MalaysiaIndustrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800 Minden, MalaysiaPhytochemistry laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800 Minden, MalaysiaA total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from Pilea microphylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at 72.10±0.71 and chloroform extract of method II (CE II) showed the highest total flavonoid contents at 60.14±0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.http://dx.doi.org/10.1155/2010/826830 |
| spellingShingle | Amir Modarresi Chahardehi Darah Ibrahim Shaida Fariza Sulaiman Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla International Journal of Microbiology |
| title | Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla |
| title_full | Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla |
| title_fullStr | Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla |
| title_full_unstemmed | Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla |
| title_short | Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla |
| title_sort | antioxidant antimicrobial activity and toxicity test of pilea microphylla |
| url | http://dx.doi.org/10.1155/2010/826830 |
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