Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction

The SD was extracted with a new green eutectic solvent, and the extraction method of TCM decoction was developed. In the quantitative analysis by HPLC, choline chloride phenol was selected as the eutectic solvent, THF was used as the extractant, and investigation of DES type, DES molar ratio, DES-to...

Full description

Saved in:
Bibliographic Details
Main Authors: Lijing Li, Yuejie Wang, Fangxin Liu, Yang Xu, Huiwei Bao
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2021/9999406
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832546259253067776
author Lijing Li
Yuejie Wang
Fangxin Liu
Yang Xu
Huiwei Bao
author_facet Lijing Li
Yuejie Wang
Fangxin Liu
Yang Xu
Huiwei Bao
author_sort Lijing Li
collection DOAJ
description The SD was extracted with a new green eutectic solvent, and the extraction method of TCM decoction was developed. In the quantitative analysis by HPLC, choline chloride phenol was selected as the eutectic solvent, THF was used as the extractant, and investigation of DES type, DES molar ratio, DES-to-THF ratio, vortex time, and material-to-liquid ratio was carried out. The experimental results showed that the optimal extraction method was as follows: the molar ratio of DES was 1 : 3, and the material-liquid ratio was 5 : 1200 (mL/μL). The volume ratio of DES to THF was 1200 : 800 (μL), the vortex time was 3 min, and the extraction was repeated two times. The eutectic solvent liquid phase microextraction method was adopted to optimize the extraction method of SD and reduce the complicated processing, long time, and low efficiency of traditional methods. At the same time, in the mouse ammonia water inducing cough and phenol red excretion and expectorant experiments, SD high- and medium-dose groups have a significant inhibitory effect on the frequency of antitussive in mice and both can increase the excretion of phenol red to varying degrees, indicating that SD has good cough-relieving and expectorant effect. The present study suggests a scientific basis and basis for the clinical research and quality standard formulation of SD.
format Article
id doaj-art-497dcc55896948aeb08086fb15226b3f
institution Kabale University
issn 2090-8865
2090-8873
language English
publishDate 2021-01-01
publisher Wiley
record_format Article
series Journal of Analytical Methods in Chemistry
spelling doaj-art-497dcc55896948aeb08086fb15226b3f2025-02-03T07:23:28ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732021-01-01202110.1155/2021/99994069999406Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi DecoctionLijing Li0Yuejie Wang1Fangxin Liu2Yang Xu3Huiwei Bao4College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, ChinaCollege of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, ChinaCollege of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, ChinaCollege of Pharmacy, Baicheng Medical College, Baicheng 137000, ChinaCollege of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, ChinaThe SD was extracted with a new green eutectic solvent, and the extraction method of TCM decoction was developed. In the quantitative analysis by HPLC, choline chloride phenol was selected as the eutectic solvent, THF was used as the extractant, and investigation of DES type, DES molar ratio, DES-to-THF ratio, vortex time, and material-to-liquid ratio was carried out. The experimental results showed that the optimal extraction method was as follows: the molar ratio of DES was 1 : 3, and the material-liquid ratio was 5 : 1200 (mL/μL). The volume ratio of DES to THF was 1200 : 800 (μL), the vortex time was 3 min, and the extraction was repeated two times. The eutectic solvent liquid phase microextraction method was adopted to optimize the extraction method of SD and reduce the complicated processing, long time, and low efficiency of traditional methods. At the same time, in the mouse ammonia water inducing cough and phenol red excretion and expectorant experiments, SD high- and medium-dose groups have a significant inhibitory effect on the frequency of antitussive in mice and both can increase the excretion of phenol red to varying degrees, indicating that SD has good cough-relieving and expectorant effect. The present study suggests a scientific basis and basis for the clinical research and quality standard formulation of SD.http://dx.doi.org/10.1155/2021/9999406
spellingShingle Lijing Li
Yuejie Wang
Fangxin Liu
Yang Xu
Huiwei Bao
Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
Journal of Analytical Methods in Chemistry
title Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
title_full Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
title_fullStr Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
title_full_unstemmed Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
title_short Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction
title_sort study on the effect of deep eutectic solvent liquid phase microextraction on quality standard antitussive and expectorant of sangbaipi decoction
url http://dx.doi.org/10.1155/2021/9999406
work_keys_str_mv AT lijingli studyontheeffectofdeepeutecticsolventliquidphasemicroextractiononqualitystandardantitussiveandexpectorantofsangbaipidecoction
AT yuejiewang studyontheeffectofdeepeutecticsolventliquidphasemicroextractiononqualitystandardantitussiveandexpectorantofsangbaipidecoction
AT fangxinliu studyontheeffectofdeepeutecticsolventliquidphasemicroextractiononqualitystandardantitussiveandexpectorantofsangbaipidecoction
AT yangxu studyontheeffectofdeepeutecticsolventliquidphasemicroextractiononqualitystandardantitussiveandexpectorantofsangbaipidecoction
AT huiweibao studyontheeffectofdeepeutecticsolventliquidphasemicroextractiononqualitystandardantitussiveandexpectorantofsangbaipidecoction