Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics

The synthesis and accumulation of bioactive compounds in crops is influenced by both environmental and genetic factors, with fertilization being a key environmental determinant. The aim of this study was to evaluate the effects of different fertilization regimes on the metabolomic profile and grain...

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Main Authors: Silvia Sánchez-Méndez, Luciano Orden, Manuel Viuda-Martos, Raquel Lucas González, Francisco Javier Andreu-Rodríguez, Miguel Ángel Mira-Urios, José Antonio Sáez-Tovar, Encarnación Martínez-Sabater, Raúl Moral
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225005578
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author Silvia Sánchez-Méndez
Luciano Orden
Manuel Viuda-Martos
Raquel Lucas González
Francisco Javier Andreu-Rodríguez
Miguel Ángel Mira-Urios
José Antonio Sáez-Tovar
Encarnación Martínez-Sabater
Raúl Moral
author_facet Silvia Sánchez-Méndez
Luciano Orden
Manuel Viuda-Martos
Raquel Lucas González
Francisco Javier Andreu-Rodríguez
Miguel Ángel Mira-Urios
José Antonio Sáez-Tovar
Encarnación Martínez-Sabater
Raúl Moral
author_sort Silvia Sánchez-Méndez
collection DOAJ
description The synthesis and accumulation of bioactive compounds in crops is influenced by both environmental and genetic factors, with fertilization being a key environmental determinant. The aim of this study was to evaluate the effects of different fertilization regimes on the metabolomic profile and grain quality of wheat, including starch content and flour colour. The treatments consisted of high phosphorus pellets at sowing (50 kg P ha−¹) and high nitrogen pellets at tillering (150 kg N ha−¹), using three types of fertilizer: (i) organic pellets (OF) derived from agro-food by-products, (ii) organic-mineral pellets (OMF) and (iii) synthetic fertilizers (IN). A control group (no fertilization) and a conventional treatment (standard synthetic NPK) were included for comparison. Metabolomic profiling was conducted using proton nuclear magnetic resonance (¹H-NMR), while the starch content and colour parameters of the flour were also measured. The results revealed that treatments combining organic and mineral fertilizers significantly increased starch content by up to 19% compared to the control and improved flour lightness (L* value). These treatments also enhanced the accumulation of key primary metabolites, including amino acids (such as glutamine and proline) and organic acids (such as malate and citrate), indicating a more favourable nutritional profile. These insights support the development of more sustainable, nutritionally focused wheat production systems.
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spelling doaj-art-496b554b1ef341f8b4c3fe04e614f10d2025-08-22T04:58:43ZengElsevierApplied Food Research2772-50222025-12-015210125210.1016/j.afres.2025.101252Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomicsSilvia Sánchez-Méndez0Luciano Orden1Manuel Viuda-Martos2Raquel Lucas González3Francisco Javier Andreu-Rodríguez4Miguel Ángel Mira-Urios5José Antonio Sáez-Tovar6Encarnación Martínez-Sabater7Raúl Moral8GIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, Spain; Corresponding author.IPOA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainIPOA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainGIAAMA Research Group, Instituto de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Miguel Hernández University, Carretera de Beniel Km 3,2, Orihuela, Alicante 03312, SpainThe synthesis and accumulation of bioactive compounds in crops is influenced by both environmental and genetic factors, with fertilization being a key environmental determinant. The aim of this study was to evaluate the effects of different fertilization regimes on the metabolomic profile and grain quality of wheat, including starch content and flour colour. The treatments consisted of high phosphorus pellets at sowing (50 kg P ha−¹) and high nitrogen pellets at tillering (150 kg N ha−¹), using three types of fertilizer: (i) organic pellets (OF) derived from agro-food by-products, (ii) organic-mineral pellets (OMF) and (iii) synthetic fertilizers (IN). A control group (no fertilization) and a conventional treatment (standard synthetic NPK) were included for comparison. Metabolomic profiling was conducted using proton nuclear magnetic resonance (¹H-NMR), while the starch content and colour parameters of the flour were also measured. The results revealed that treatments combining organic and mineral fertilizers significantly increased starch content by up to 19% compared to the control and improved flour lightness (L* value). These treatments also enhanced the accumulation of key primary metabolites, including amino acids (such as glutamine and proline) and organic acids (such as malate and citrate), indicating a more favourable nutritional profile. These insights support the development of more sustainable, nutritionally focused wheat production systems.http://www.sciencedirect.com/science/article/pii/S2772502225005578Sustainable fertilizationMetabolomicsWheatCompost1H-NMRGrain quality
spellingShingle Silvia Sánchez-Méndez
Luciano Orden
Manuel Viuda-Martos
Raquel Lucas González
Francisco Javier Andreu-Rodríguez
Miguel Ángel Mira-Urios
José Antonio Sáez-Tovar
Encarnación Martínez-Sabater
Raúl Moral
Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
Applied Food Research
Sustainable fertilization
Metabolomics
Wheat
Compost
1H-NMR
Grain quality
title Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
title_full Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
title_fullStr Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
title_full_unstemmed Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
title_short Effect of different biofertilization strategies on wheat (Triticum turgidum) flour metabolomics
title_sort effect of different biofertilization strategies on wheat triticum turgidum flour metabolomics
topic Sustainable fertilization
Metabolomics
Wheat
Compost
1H-NMR
Grain quality
url http://www.sciencedirect.com/science/article/pii/S2772502225005578
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