Molecular Diversity of Arbuscular Mycorrhizal Fungi Associated with the Rhizosphere of <i>Vachellia seyal</i> Del. from Selected Saline Soils in Senegal
Drought and salinity are major environmental constraints that severely limit crop production, particularly in arid and semi-arid zones. We investigated the genetic diversity of arbuscular mycorrhizal fungi isolated from the rhizosphere of <i>Vachellia seyal</i> in three different soils f...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2025-01-01
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Series: | Microbiology Research |
Subjects: | |
Online Access: | https://www.mdpi.com/2036-7481/16/1/19 |
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Summary: | Drought and salinity are major environmental constraints that severely limit crop production, particularly in arid and semi-arid zones. We investigated the genetic diversity of arbuscular mycorrhizal fungi isolated from the rhizosphere of <i>Vachellia seyal</i> in three different soils from Senegal with varying levels of salinity. Soil and root samples were collected from under <i>V. seyal</i> and in the vicinity of the trees. After DNA extraction, nested PCR, and sequencing of the large subunit region of the rRNA gene, different phylotypes from rhizospheric soils, roots, and spores were compared by phylogeny in order to investigate the role of salinity in arbuscular fungal diversity. This study revealed several unidentified arbuscular fungi and a particularly high host specificity in <i>V. seyal</i> roots. The vast majority of operational taxonomic units (OTUs) isolated in this study had no homologous sequences in the databases. |
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ISSN: | 2036-7481 |