Preanalytical factors affecting the plasma and serum levels of circulating microRNAs
Circulating microribonucleic acids (microRNAs) are promising biomarkers of various diseases, but their clinical laboratory use requires highly sensitive, reproducible, reliable and sustainable methods for their accurate plasma and serum quantification. The preanalytical phase of studies conducted us...
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| Format: | Article |
| Language: | Russian |
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«SILICEA-POLIGRAF» LLC
2025-01-01
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| Series: | Кардиоваскулярная терапия и профилактика |
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| Online Access: | https://cardiovascular.elpub.ru/jour/article/view/4179 |
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| author | E. A. Sotnikova A. V. Kiseleva A. N. Meshkov |
| author_facet | E. A. Sotnikova A. V. Kiseleva A. N. Meshkov |
| author_sort | E. A. Sotnikova |
| collection | DOAJ |
| description | Circulating microribonucleic acids (microRNAs) are promising biomarkers of various diseases, but their clinical laboratory use requires highly sensitive, reproducible, reliable and sustainable methods for their accurate plasma and serum quantification. The preanalytical phase of studies conducted using biospecimens consists of their collection, processing, storage and transportation. Preanalytical conditions remain the main distorting factors in microRNA studies, and standardization of these conditions, carried out in biobanks, can improve the reproducibility of results and their comparison. The review aim is to consider the main contemporary original studies on preanalytical factors, which are an important source of variability in studies on circulating microRNAs at the stages from blood collection to plasma or serum production. |
| format | Article |
| id | doaj-art-49525b8d2df640caa3f93db0615a21ae |
| institution | Kabale University |
| issn | 1728-8800 2619-0125 |
| language | Russian |
| publishDate | 2025-01-01 |
| publisher | «SILICEA-POLIGRAF» LLC |
| record_format | Article |
| series | Кардиоваскулярная терапия и профилактика |
| spelling | doaj-art-49525b8d2df640caa3f93db0615a21ae2025-08-20T03:57:18Zrus«SILICEA-POLIGRAF» LLCКардиоваскулярная терапия и профилактика1728-88002619-01252025-01-01231110.15829/1728-8800-2024-41793111Preanalytical factors affecting the plasma and serum levels of circulating microRNAsE. A. Sotnikova0A. V. Kiseleva1A. N. Meshkov2National Medical Research Center for Therapy and Preventive MedicineNational Medical Research Center for Therapy and Preventive MedicineNational Medical Research Center for Therapy and Preventive MedicineCirculating microribonucleic acids (microRNAs) are promising biomarkers of various diseases, but their clinical laboratory use requires highly sensitive, reproducible, reliable and sustainable methods for their accurate plasma and serum quantification. The preanalytical phase of studies conducted using biospecimens consists of their collection, processing, storage and transportation. Preanalytical conditions remain the main distorting factors in microRNA studies, and standardization of these conditions, carried out in biobanks, can improve the reproducibility of results and their comparison. The review aim is to consider the main contemporary original studies on preanalytical factors, which are an important source of variability in studies on circulating microRNAs at the stages from blood collection to plasma or serum production.https://cardiovascular.elpub.ru/jour/article/view/4179micrornapreanalytical factorsplasmaserumcentrifugationanticoagulantsblood storage |
| spellingShingle | E. A. Sotnikova A. V. Kiseleva A. N. Meshkov Preanalytical factors affecting the plasma and serum levels of circulating microRNAs Кардиоваскулярная терапия и профилактика microrna preanalytical factors plasma serum centrifugation anticoagulants blood storage |
| title | Preanalytical factors affecting the plasma and serum levels of circulating microRNAs |
| title_full | Preanalytical factors affecting the plasma and serum levels of circulating microRNAs |
| title_fullStr | Preanalytical factors affecting the plasma and serum levels of circulating microRNAs |
| title_full_unstemmed | Preanalytical factors affecting the plasma and serum levels of circulating microRNAs |
| title_short | Preanalytical factors affecting the plasma and serum levels of circulating microRNAs |
| title_sort | preanalytical factors affecting the plasma and serum levels of circulating micrornas |
| topic | microrna preanalytical factors plasma serum centrifugation anticoagulants blood storage |
| url | https://cardiovascular.elpub.ru/jour/article/view/4179 |
| work_keys_str_mv | AT easotnikova preanalyticalfactorsaffectingtheplasmaandserumlevelsofcirculatingmicrornas AT avkiseleva preanalyticalfactorsaffectingtheplasmaandserumlevelsofcirculatingmicrornas AT anmeshkov preanalyticalfactorsaffectingtheplasmaandserumlevelsofcirculatingmicrornas |