Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia

Abstract With high disability and mortality rate as well as highly complex pathogenesis, cerebral ischemia is highly morbid, prone to recurrence. To comprehensively understand the pathophysiological process of cerebral ischemia and to find new therapeutic strategies, a new approach to cerebral ische...

Full description

Saved in:
Bibliographic Details
Main Authors: Jiaming Liu, Tao Qing, Mei he, Liu Xu, Zhuxiang Wu, Meiting Huang, Zheyu Liu, Ye Zhang, Zisheng Li, Wenhui Yang, Junbo Liu, Jie Li
Format: Article
Language:English
Published: BMC 2025-04-01
Series:European Journal of Medical Research
Subjects:
Online Access:https://doi.org/10.1186/s40001-025-02596-2
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849713027320905728
author Jiaming Liu
Tao Qing
Mei he
Liu Xu
Zhuxiang Wu
Meiting Huang
Zheyu Liu
Ye Zhang
Zisheng Li
Wenhui Yang
Junbo Liu
Jie Li
author_facet Jiaming Liu
Tao Qing
Mei he
Liu Xu
Zhuxiang Wu
Meiting Huang
Zheyu Liu
Ye Zhang
Zisheng Li
Wenhui Yang
Junbo Liu
Jie Li
author_sort Jiaming Liu
collection DOAJ
description Abstract With high disability and mortality rate as well as highly complex pathogenesis, cerebral ischemia is highly morbid, prone to recurrence. To comprehensively understand the pathophysiological process of cerebral ischemia and to find new therapeutic strategies, a new approach to cerebral ischemia transcriptomics has emerged in recent years. By integrating data from multiple levels of transcriptomics, such as transcriptomics, single-cell transcriptomics, and spatial transcriptomics, this new approach can provide powerful help in revealing the molecular mechanisms of cerebral ischemia occurrence and development. Key findings highlight the critical roles of inflammation, blood–brain barrier dysfunction, and mitochondrial dysregulation in cerebral ischemia, offering potential biomarkers and therapeutic targets for early diagnosis and personalized treatment. A review of the research progress of cerebral ischemic injury mechanism under the analysis of the comprehensive transcriptomics research method was presented in this article, aiming to study the potential mechanism to provide new, innovative therapeutic strategies for this disease.
format Article
id doaj-art-493ac38e597f4f019abdcf3d3b4bc761
institution DOAJ
issn 2047-783X
language English
publishDate 2025-04-01
publisher BMC
record_format Article
series European Journal of Medical Research
spelling doaj-art-493ac38e597f4f019abdcf3d3b4bc7612025-08-20T03:14:05ZengBMCEuropean Journal of Medical Research2047-783X2025-04-0130111810.1186/s40001-025-02596-2Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemiaJiaming Liu0Tao Qing1Mei he2Liu Xu3Zhuxiang Wu4Meiting Huang5Zheyu Liu6Ye Zhang7Zisheng Li8Wenhui Yang9Junbo Liu10Jie Li11Basic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineHunan Provincial Maternal and Child Health Care HospitalInternational Education School, Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineBasic Medical College of Hunan University of MedicineAbstract With high disability and mortality rate as well as highly complex pathogenesis, cerebral ischemia is highly morbid, prone to recurrence. To comprehensively understand the pathophysiological process of cerebral ischemia and to find new therapeutic strategies, a new approach to cerebral ischemia transcriptomics has emerged in recent years. By integrating data from multiple levels of transcriptomics, such as transcriptomics, single-cell transcriptomics, and spatial transcriptomics, this new approach can provide powerful help in revealing the molecular mechanisms of cerebral ischemia occurrence and development. Key findings highlight the critical roles of inflammation, blood–brain barrier dysfunction, and mitochondrial dysregulation in cerebral ischemia, offering potential biomarkers and therapeutic targets for early diagnosis and personalized treatment. A review of the research progress of cerebral ischemic injury mechanism under the analysis of the comprehensive transcriptomics research method was presented in this article, aiming to study the potential mechanism to provide new, innovative therapeutic strategies for this disease.https://doi.org/10.1186/s40001-025-02596-2Cerebral ischemiaTranscriptomicsSingle-cell transcriptomicsSpatial transcriptomicsMulti-omics study
spellingShingle Jiaming Liu
Tao Qing
Mei he
Liu Xu
Zhuxiang Wu
Meiting Huang
Zheyu Liu
Ye Zhang
Zisheng Li
Wenhui Yang
Junbo Liu
Jie Li
Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
European Journal of Medical Research
Cerebral ischemia
Transcriptomics
Single-cell transcriptomics
Spatial transcriptomics
Multi-omics study
title Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
title_full Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
title_fullStr Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
title_full_unstemmed Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
title_short Transcriptomics, single-cell sequencing and spatial sequencing-based studies of cerebral ischemia
title_sort transcriptomics single cell sequencing and spatial sequencing based studies of cerebral ischemia
topic Cerebral ischemia
Transcriptomics
Single-cell transcriptomics
Spatial transcriptomics
Multi-omics study
url https://doi.org/10.1186/s40001-025-02596-2
work_keys_str_mv AT jiamingliu transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT taoqing transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT meihe transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT liuxu transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT zhuxiangwu transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT meitinghuang transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT zheyuliu transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT yezhang transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT zishengli transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT wenhuiyang transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT junboliu transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia
AT jieli transcriptomicssinglecellsequencingandspatialsequencingbasedstudiesofcerebralischemia