Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases

Crohn’s disease (CD) and ulcerative colitis (UC) belong to a group of inflammatory bowel diseases (IBD). The aim of our study was to evaluate the expression of MMP-2, MMP-7, MMP-9, TIMP-1, and TIMP-2 in ulcerative colitis and Crohn’s disease. The study group comprised 34 patients with UC and 10 pati...

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Main Authors: Katarzyna Jakubowska, Anna Pryczynicz, Piotr Iwanowicz, Andrzej Niewiński, Elżbieta Maciorkowska, Jerzy Hapanowicz, Dorota Jagodzińska, Andrzej Kemona, Katarzyna Guzińska-Ustymowicz
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Gastroenterology Research and Practice
Online Access:http://dx.doi.org/10.1155/2016/2456179
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author Katarzyna Jakubowska
Anna Pryczynicz
Piotr Iwanowicz
Andrzej Niewiński
Elżbieta Maciorkowska
Jerzy Hapanowicz
Dorota Jagodzińska
Andrzej Kemona
Katarzyna Guzińska-Ustymowicz
author_facet Katarzyna Jakubowska
Anna Pryczynicz
Piotr Iwanowicz
Andrzej Niewiński
Elżbieta Maciorkowska
Jerzy Hapanowicz
Dorota Jagodzińska
Andrzej Kemona
Katarzyna Guzińska-Ustymowicz
author_sort Katarzyna Jakubowska
collection DOAJ
description Crohn’s disease (CD) and ulcerative colitis (UC) belong to a group of inflammatory bowel diseases (IBD). The aim of our study was to evaluate the expression of MMP-2, MMP-7, MMP-9, TIMP-1, and TIMP-2 in ulcerative colitis and Crohn’s disease. The study group comprised 34 patients with UC and 10 patients with CD. Evaluation of MMP-2, MMP-7, MMP-9, TIMP-1, and TIMP-2 expression in tissue samples was performed using immunohistochemistry. The overexpression of MMP-9 and TIMP-1 was dominant in both the glandular epithelium and inflammatory infiltration in UC patients. In contrast, in CD subjects the positive expression of MMP-2 and TIMP-1 was in glandular tubes while mainly MMP-7 and TIMP-2 expression was in inflammatory infiltration. Metalloproteinases’ expression was associated with the presence of erosions, architectural tissue changes, and inflammatory infiltration in the lamina propria of UC patients. The expression of metalloproteinase inhibitors correlated with the presence of eosinophils and neutrophils in UC and granulomas in CD patients. Our studies indicate that the overexpression of metalloproteinases and weaker expression of their inhibitors may determine the development of IBD. It appears that MMP-2, MMP-7, and MMP-9 may be a potential therapeutic target and the use of their inhibitors may significantly reduce UC progression.
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spelling doaj-art-491e4c494ce14ee28af08c55d61b8b722025-08-20T02:05:56ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/24561792456179Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel DiseasesKatarzyna Jakubowska0Anna Pryczynicz1Piotr Iwanowicz2Andrzej Niewiński3Elżbieta Maciorkowska4Jerzy Hapanowicz5Dorota Jagodzińska6Andrzej Kemona7Katarzyna Guzińska-Ustymowicz8Department of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandDepartment of Rehabilitation, Medical University of Bialystok, Bialystok, PolandDepartment of Developmental Age Medicine and Paediatric Nursing, Medical University of Bialystok, Bialystok, PolandDepartment of Oncological Surgery, Comprehensive Cancer Centre, Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, Waszyngtona Street 13, 15-269 Bialystok, PolandCrohn’s disease (CD) and ulcerative colitis (UC) belong to a group of inflammatory bowel diseases (IBD). The aim of our study was to evaluate the expression of MMP-2, MMP-7, MMP-9, TIMP-1, and TIMP-2 in ulcerative colitis and Crohn’s disease. The study group comprised 34 patients with UC and 10 patients with CD. Evaluation of MMP-2, MMP-7, MMP-9, TIMP-1, and TIMP-2 expression in tissue samples was performed using immunohistochemistry. The overexpression of MMP-9 and TIMP-1 was dominant in both the glandular epithelium and inflammatory infiltration in UC patients. In contrast, in CD subjects the positive expression of MMP-2 and TIMP-1 was in glandular tubes while mainly MMP-7 and TIMP-2 expression was in inflammatory infiltration. Metalloproteinases’ expression was associated with the presence of erosions, architectural tissue changes, and inflammatory infiltration in the lamina propria of UC patients. The expression of metalloproteinase inhibitors correlated with the presence of eosinophils and neutrophils in UC and granulomas in CD patients. Our studies indicate that the overexpression of metalloproteinases and weaker expression of their inhibitors may determine the development of IBD. It appears that MMP-2, MMP-7, and MMP-9 may be a potential therapeutic target and the use of their inhibitors may significantly reduce UC progression.http://dx.doi.org/10.1155/2016/2456179
spellingShingle Katarzyna Jakubowska
Anna Pryczynicz
Piotr Iwanowicz
Andrzej Niewiński
Elżbieta Maciorkowska
Jerzy Hapanowicz
Dorota Jagodzińska
Andrzej Kemona
Katarzyna Guzińska-Ustymowicz
Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
Gastroenterology Research and Practice
title Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
title_full Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
title_fullStr Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
title_full_unstemmed Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
title_short Expressions of Matrix Metalloproteinases (MMP-2, MMP-7, and MMP-9) and Their Inhibitors (TIMP-1, TIMP-2) in Inflammatory Bowel Diseases
title_sort expressions of matrix metalloproteinases mmp 2 mmp 7 and mmp 9 and their inhibitors timp 1 timp 2 in inflammatory bowel diseases
url http://dx.doi.org/10.1155/2016/2456179
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