Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov.
The genus <i>Haloarcula</i> is one of the most extensively studied genera within the class <i>Halobacteria</i>. In this study, analyses of average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA–DNA hybridization (dDDH) values, and phylogenomic data...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/14/6/615 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849418349791936512 |
|---|---|
| author | Ji-Qiang Liu Ling-Rui Zhu Ya-Ling Mao Xue Ma Jing Hou |
| author_facet | Ji-Qiang Liu Ling-Rui Zhu Ya-Ling Mao Xue Ma Jing Hou |
| author_sort | Ji-Qiang Liu |
| collection | DOAJ |
| description | The genus <i>Haloarcula</i> is one of the most extensively studied genera within the class <i>Halobacteria</i>. In this study, analyses of average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA–DNA hybridization (dDDH) values, and phylogenomic data indicated that “<i>Haloarcula californiae</i>” ATCC 33799 and “<i>Haloarcula sinaiiensis</i>” ATCC 33800 served as reference strains for <i>Haloarcula marismortui</i>. Furthermore, a halophilic archaeal strain, GH36<sup>T</sup>, isolated from the inland Gahai Salt Lake in China, was subjected to comprehensive polyphasic taxonomic characterization. The ANI, AAI, and dDDH comparisons between strain GH36<sup>T</sup> and other <i>Haloarcula</i> species were below the established thresholds for species demarcation. Strain GH36<sup>T</sup> was assigned to a novel species within the genus <i>Haloarcula</i> based on phylogenetic, phylogenomic, and comparative genomic analyses. Moreover, distinct phenotypic differences were observed in strain GH36<sup>T</sup>. In strain GH36<sup>T</sup>, the primary phospholipids detected were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), and phosphatidylglycerol sulfate (PGS), whereas the principal glycolipids were sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). Accordingly, the novel species <i>Haloarcula montana</i> sp. nov. is proposed to accommodate strain GH36<sup>T</sup> (=CGMCC 1.62631<sup>T</sup> = MCCC 4K00122<sup>T</sup>). |
| format | Article |
| id | doaj-art-490cb3b67ca543969837a5f983a970da |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-490cb3b67ca543969837a5f983a970da2025-08-20T03:32:27ZengMDPI AGBiology2079-77372025-05-0114661510.3390/biology14060615Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov.Ji-Qiang Liu0Ling-Rui Zhu1Ya-Ling Mao2Xue Ma3Jing Hou4State Key Laboratory of Submarine Geoscience, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaThe genus <i>Haloarcula</i> is one of the most extensively studied genera within the class <i>Halobacteria</i>. In this study, analyses of average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA–DNA hybridization (dDDH) values, and phylogenomic data indicated that “<i>Haloarcula californiae</i>” ATCC 33799 and “<i>Haloarcula sinaiiensis</i>” ATCC 33800 served as reference strains for <i>Haloarcula marismortui</i>. Furthermore, a halophilic archaeal strain, GH36<sup>T</sup>, isolated from the inland Gahai Salt Lake in China, was subjected to comprehensive polyphasic taxonomic characterization. The ANI, AAI, and dDDH comparisons between strain GH36<sup>T</sup> and other <i>Haloarcula</i> species were below the established thresholds for species demarcation. Strain GH36<sup>T</sup> was assigned to a novel species within the genus <i>Haloarcula</i> based on phylogenetic, phylogenomic, and comparative genomic analyses. Moreover, distinct phenotypic differences were observed in strain GH36<sup>T</sup>. In strain GH36<sup>T</sup>, the primary phospholipids detected were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), and phosphatidylglycerol sulfate (PGS), whereas the principal glycolipids were sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). Accordingly, the novel species <i>Haloarcula montana</i> sp. nov. is proposed to accommodate strain GH36<sup>T</sup> (=CGMCC 1.62631<sup>T</sup> = MCCC 4K00122<sup>T</sup>).https://www.mdpi.com/2079-7737/14/6/615<i>Haloarcula</i>novel halophilic archaeonsalt lakecomparative genomicstaxogenomics |
| spellingShingle | Ji-Qiang Liu Ling-Rui Zhu Ya-Ling Mao Xue Ma Jing Hou Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. Biology <i>Haloarcula</i> novel halophilic archaeon salt lake comparative genomics taxogenomics |
| title | Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. |
| title_full | Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. |
| title_fullStr | Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. |
| title_full_unstemmed | Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. |
| title_short | Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov. |
| title_sort | genome based reclassification of two i haloarcula i species and characterization of i haloarcula montana i sp nov |
| topic | <i>Haloarcula</i> novel halophilic archaeon salt lake comparative genomics taxogenomics |
| url | https://www.mdpi.com/2079-7737/14/6/615 |
| work_keys_str_mv | AT jiqiangliu genomebasedreclassificationoftwoihaloarculaispeciesandcharacterizationofihaloarculamontanaispnov AT lingruizhu genomebasedreclassificationoftwoihaloarculaispeciesandcharacterizationofihaloarculamontanaispnov AT yalingmao genomebasedreclassificationoftwoihaloarculaispeciesandcharacterizationofihaloarculamontanaispnov AT xuema genomebasedreclassificationoftwoihaloarculaispeciesandcharacterizationofihaloarculamontanaispnov AT jinghou genomebasedreclassificationoftwoihaloarculaispeciesandcharacterizationofihaloarculamontanaispnov |