Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov.

Genome-Based Reclassification of Two <i>Haloarcula</i> Species and Characterization of <i>Haloarcula montana</i> sp. nov.

The genus <i>Haloarcula</i> is one of the most extensively studied genera within the class <i>Halobacteria</i>. In this study, analyses of average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA–DNA hybridization (dDDH) values, and phylogenomic data...

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Bibliographic Details
Main Authors: Ji-Qiang Liu, Ling-Rui Zhu, Ya-Ling Mao, Xue Ma, Jing Hou
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Biology
Subjects:
<i>Haloarcula</i>
novel halophilic archaeon
salt lake
comparative genomics
taxogenomics
Online Access:https://www.mdpi.com/2079-7737/14/6/615
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Summary:The genus <i>Haloarcula</i> is one of the most extensively studied genera within the class <i>Halobacteria</i>. In this study, analyses of average nucleotide identity (ANI), average amino acid identity (AAI), digital DNA–DNA hybridization (dDDH) values, and phylogenomic data indicated that “<i>Haloarcula californiae</i>” ATCC 33799 and “<i>Haloarcula sinaiiensis</i>” ATCC 33800 served as reference strains for <i>Haloarcula marismortui</i>. Furthermore, a halophilic archaeal strain, GH36<sup>T</sup>, isolated from the inland Gahai Salt Lake in China, was subjected to comprehensive polyphasic taxonomic characterization. The ANI, AAI, and dDDH comparisons between strain GH36<sup>T</sup> and other <i>Haloarcula</i> species were below the established thresholds for species demarcation. Strain GH36<sup>T</sup> was assigned to a novel species within the genus <i>Haloarcula</i> based on phylogenetic, phylogenomic, and comparative genomic analyses. Moreover, distinct phenotypic differences were observed in strain GH36<sup>T</sup>. In strain GH36<sup>T</sup>, the primary phospholipids detected were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), and phosphatidylglycerol sulfate (PGS), whereas the principal glycolipids were sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). Accordingly, the novel species <i>Haloarcula montana</i> sp. nov. is proposed to accommodate strain GH36<sup>T</sup> (=CGMCC 1.62631<sup>T</sup> = MCCC 4K00122<sup>T</sup>).
ISSN:2079-7737

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