Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa
Abstract The fall armyworm Spodoptera frugiperda (J.E. Smith) is a new serious destructive and widespread pest of corn which recently invaded subtropical regions worldwide. As this invasive species is spreading across the continent, it is vital to assess its susceptibility to currently used insectic...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-99196-1 |
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| author | Eric Tossou Ghislain T. Tepa-Yotto Georg Goergen Genevieve M. Tchigossou Magellan Tchouakui Daniel Nguiffo Nguete Laouali Amadou Moussa Noussourou Aimé H. Bokonon-Ganta Charles Wondji Manuele Tamò Rousseau Djouaka |
| author_facet | Eric Tossou Ghislain T. Tepa-Yotto Georg Goergen Genevieve M. Tchigossou Magellan Tchouakui Daniel Nguiffo Nguete Laouali Amadou Moussa Noussourou Aimé H. Bokonon-Ganta Charles Wondji Manuele Tamò Rousseau Djouaka |
| author_sort | Eric Tossou |
| collection | DOAJ |
| description | Abstract The fall armyworm Spodoptera frugiperda (J.E. Smith) is a new serious destructive and widespread pest of corn which recently invaded subtropical regions worldwide. As this invasive species is spreading across the continent, it is vital to assess its susceptibility to currently used insecticides and establish the potential underlying resistance mechanism to better inform control programmes. In this study, we characterized the strains from eighteen fall armyworm populations from different countries in West Africa, established their susceptibility profiles to the main insecticides and genotyped the target site resistance alleles. The RFLP-PCR method showed that the majority of the population tested was a corn strain (72.5–100%) compared to the probable rice strain (0–27.5%). Tpi sequencing of the suspected rice strains revealed that almost all the samples analysed were from corn (> 97%). Additionally, the three insecticides tested, λ-cyhalothrin, chlorpyrifos and emamectin benzoate induced susceptible to moderate toxicity against this pest. Synergism tests performed to investigate the biochemical mechanism used by fall armyworm to breakdown λ-cyhalothrin indicated that metabolic enzymes (oxydases, esterase and the glutathion-S-transferase) play moderate roles in the resistance of λ-cyhalothrin observed in western Africa. Target mutation tests (qPCR) combined with previous synergetic tests showed that resistance to organophosphates and pyrethroids could be due to a biochemical mechanism + amino acid mutations (presence of the F290V mutation) or a biochemical mechanism (absence of the T929I mutation), respectively. The results of this study provide valuable information for supporting decisions related to sustainable fall armyworm control and applied resistance management. |
| format | Article |
| id | doaj-art-490b72392ae14b3db8bbd519f747d0f2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-490b72392ae14b3db8bbd519f747d0f22025-08-20T04:01:26ZengNature PortfolioScientific Reports2045-23222025-07-0115111610.1038/s41598-025-99196-1Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West AfricaEric Tossou0Ghislain T. Tepa-Yotto1Georg Goergen2Genevieve M. Tchigossou3Magellan Tchouakui4Daniel Nguiffo Nguete5Laouali Amadou6Moussa Noussourou7Aimé H. Bokonon-Ganta8Charles Wondji9Manuele Tamò10Rousseau Djouaka11Agroecohealth Unit , International Institute of Tropical Agriculture (IITA) Biorisk Management Facility (BIMAF), International Institute of Tropical Agriculture (IITA-Benin)Biorisk Management Facility (BIMAF), International Institute of Tropical Agriculture (IITA-Benin)Agroecohealth Unit , International Institute of Tropical Agriculture (IITA) Centre for Research in Infectious Diseases (CRID)Centre for Research in Infectious Diseases (CRID)National Institute of Agricultural Research (INRAN)Institute of Rural Economy (IER)Laboratory of Agricultural Entomology (LEAg), Faculty of Agronomic Sciences (FSA), University of Abomey-Calavi (UAC)Centre for Research in Infectious Diseases (CRID)Biorisk Management Facility (BIMAF), International Institute of Tropical Agriculture (IITA-Benin)Agroecohealth Unit , International Institute of Tropical Agriculture (IITA) Abstract The fall armyworm Spodoptera frugiperda (J.E. Smith) is a new serious destructive and widespread pest of corn which recently invaded subtropical regions worldwide. As this invasive species is spreading across the continent, it is vital to assess its susceptibility to currently used insecticides and establish the potential underlying resistance mechanism to better inform control programmes. In this study, we characterized the strains from eighteen fall armyworm populations from different countries in West Africa, established their susceptibility profiles to the main insecticides and genotyped the target site resistance alleles. The RFLP-PCR method showed that the majority of the population tested was a corn strain (72.5–100%) compared to the probable rice strain (0–27.5%). Tpi sequencing of the suspected rice strains revealed that almost all the samples analysed were from corn (> 97%). Additionally, the three insecticides tested, λ-cyhalothrin, chlorpyrifos and emamectin benzoate induced susceptible to moderate toxicity against this pest. Synergism tests performed to investigate the biochemical mechanism used by fall armyworm to breakdown λ-cyhalothrin indicated that metabolic enzymes (oxydases, esterase and the glutathion-S-transferase) play moderate roles in the resistance of λ-cyhalothrin observed in western Africa. Target mutation tests (qPCR) combined with previous synergetic tests showed that resistance to organophosphates and pyrethroids could be due to a biochemical mechanism + amino acid mutations (presence of the F290V mutation) or a biochemical mechanism (absence of the T929I mutation), respectively. The results of this study provide valuable information for supporting decisions related to sustainable fall armyworm control and applied resistance management.https://doi.org/10.1038/s41598-025-99196-1Fall armywormInsecticide resistanceTarget site mutationsC-strainR-strainResistance management |
| spellingShingle | Eric Tossou Ghislain T. Tepa-Yotto Georg Goergen Genevieve M. Tchigossou Magellan Tchouakui Daniel Nguiffo Nguete Laouali Amadou Moussa Noussourou Aimé H. Bokonon-Ganta Charles Wondji Manuele Tamò Rousseau Djouaka Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa Scientific Reports Fall armyworm Insecticide resistance Target site mutations C-strain R-strain Resistance management |
| title | Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa |
| title_full | Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa |
| title_fullStr | Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa |
| title_full_unstemmed | Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa |
| title_short | Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa |
| title_sort | genetic variation associated with increased lambda cyhalothrin resistance in spodoptera frugiperda lepidoptera noctuidae in west africa |
| topic | Fall armyworm Insecticide resistance Target site mutations C-strain R-strain Resistance management |
| url | https://doi.org/10.1038/s41598-025-99196-1 |
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