Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum

Abstract Microbial biogeography and its controlling mechanisms are central themes in microbial ecology. However, we still lack a comprehensive understanding of how habitats and lifestyles affect microbial biogeography across complex environmental gradients. In this study, we investigated the plankto...

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Main Authors: Jiao Liu, Peng Yao, Jinmei Liu, Gaoyang Ren, Xiao‐Hua Zhang, Jiwen Liu
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:mLife
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Online Access:https://doi.org/10.1002/mlf2.70017
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author Jiao Liu
Peng Yao
Jinmei Liu
Gaoyang Ren
Xiao‐Hua Zhang
Jiwen Liu
author_facet Jiao Liu
Peng Yao
Jinmei Liu
Gaoyang Ren
Xiao‐Hua Zhang
Jiwen Liu
author_sort Jiao Liu
collection DOAJ
description Abstract Microbial biogeography and its controlling mechanisms are central themes in microbial ecology. However, we still lack a comprehensive understanding of how habitats and lifestyles affect microbial biogeography across complex environmental gradients. In this study, we investigated the planktonic (including free‐living [FL] and particle‐associated [PA] lifestyles) and benthic prokaryotic communities along a river–estuary–sea continuum of the Changjiang River to explore their distinct spatial dynamics. We observed greater community variability across spatial distances than between habitat and lifestyle types. Spatial variations were evident in FL, PA, and benthic communities, with the highest turnover rates observed in benthic communities, followed by PA, and the lowest turnover rates observed in FL. The replacement effect dominated PA and benthic community variations, whereas the richness effect was more significant in FL communities. Microbial assembly was primarily governed by homogeneous selection and dispersal limitation regardless of habitats/lifestyles, with their ratios decreasing as the spatial distance increased, particularly in the FL fraction. Dispersal limitation had a stronger effect on benthic communities compared to planktonic communities. While heterogeneous selection generally played a minor role, its influence became more pronounced over larger spatial distances and with increasing salinity differences. Finally, we showed that abiotic and biotic factors individually exerted a greater influence on PA communities, whereas their interactions had a stronger effect on FL communities. Our results revealed complex spatial dynamics and assembly mechanisms among microorganisms across different habitats and lifestyles, providing insights into the spatial scaling of community assembly across complex environmental gradients.
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spelling doaj-art-49012d6be52041fd8ae591a0ab4da2502025-08-20T03:31:14ZengWileymLife2770-100X2025-06-014330531810.1002/mlf2.70017Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuumJiao Liu0Peng Yao1Jinmei Liu2Gaoyang Ren3Xiao‐Hua Zhang4Jiwen Liu5College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of China Qingdao ChinaLaboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao ChinaCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of China Qingdao ChinaCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of China Qingdao ChinaCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of China Qingdao ChinaCollege of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System Ocean University of China Qingdao ChinaAbstract Microbial biogeography and its controlling mechanisms are central themes in microbial ecology. However, we still lack a comprehensive understanding of how habitats and lifestyles affect microbial biogeography across complex environmental gradients. In this study, we investigated the planktonic (including free‐living [FL] and particle‐associated [PA] lifestyles) and benthic prokaryotic communities along a river–estuary–sea continuum of the Changjiang River to explore their distinct spatial dynamics. We observed greater community variability across spatial distances than between habitat and lifestyle types. Spatial variations were evident in FL, PA, and benthic communities, with the highest turnover rates observed in benthic communities, followed by PA, and the lowest turnover rates observed in FL. The replacement effect dominated PA and benthic community variations, whereas the richness effect was more significant in FL communities. Microbial assembly was primarily governed by homogeneous selection and dispersal limitation regardless of habitats/lifestyles, with their ratios decreasing as the spatial distance increased, particularly in the FL fraction. Dispersal limitation had a stronger effect on benthic communities compared to planktonic communities. While heterogeneous selection generally played a minor role, its influence became more pronounced over larger spatial distances and with increasing salinity differences. Finally, we showed that abiotic and biotic factors individually exerted a greater influence on PA communities, whereas their interactions had a stronger effect on FL communities. Our results revealed complex spatial dynamics and assembly mechanisms among microorganisms across different habitats and lifestyles, providing insights into the spatial scaling of community assembly across complex environmental gradients.https://doi.org/10.1002/mlf2.70017Changjiang Rivercommunity assemblyhabitatmicrobial communityriver–estuary–sea continuum
spellingShingle Jiao Liu
Peng Yao
Jinmei Liu
Gaoyang Ren
Xiao‐Hua Zhang
Jiwen Liu
Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
mLife
Changjiang River
community assembly
habitat
microbial community
river–estuary–sea continuum
title Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
title_full Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
title_fullStr Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
title_full_unstemmed Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
title_short Habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river–estuary–sea continuum
title_sort habitat and lifestyle affect the spatial dynamics of prokaryotic communities along a river estuary sea continuum
topic Changjiang River
community assembly
habitat
microbial community
river–estuary–sea continuum
url https://doi.org/10.1002/mlf2.70017
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