Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells
Background. Oxidative stress induced by reactive oxygen and nitrogen species is critically involved in the impairment of β-cell function during the development of diabetes. Methods. HIT-T15 cells were cultured in 5% CO2 and then preincubated with Gelam honey extracts (20, 40, 60, and 80 µg/mL) as we...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/367312 |
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| author | Kalaivani Batumalaie Sher Zaman Safi Kamaruddin Mohd Yusof Ikram Shah Ismail Shamala Devi Sekaran Rajes Qvist |
| author_facet | Kalaivani Batumalaie Sher Zaman Safi Kamaruddin Mohd Yusof Ikram Shah Ismail Shamala Devi Sekaran Rajes Qvist |
| author_sort | Kalaivani Batumalaie |
| collection | DOAJ |
| description | Background. Oxidative stress induced by reactive oxygen and nitrogen species is critically involved in the impairment of β-cell function during the development of diabetes. Methods. HIT-T15 cells were cultured in 5% CO2 and then preincubated with Gelam honey extracts (20, 40, 60, and 80 µg/mL) as well as quercetin (20, 40, 60, and 80 µM), prior to stimulation by 20 and 50 mM of glucose. Cell lysate was collected to determine the effect of honey extracts and quercetin on the stress activated NF-κB, MAPK pathways, and the Akt (ser473) activated insulin signaling pathway. Results. HIT-T15 cells cultured under hyperglycemic conditions demonstrated insulin resistance with a significant increase in the levels of MAPK, NF-κB, and IRS-1 serine phosphorylation (ser307); however, Akt expression and insulin contents are significantly decreased. Pretreatment with quercetin and Gelam honey extract improved insulin resistance and insulin content by reducing the expression of MAPK, NF-κB, and IRS-1 serine phosphorylation (ser307) and increasing the expression of Akt significantly. Conclusion. Gelam honey-induced differential expression of MAPK, NF-κB, IRS-1 (ser307), and Akt in HIT-T15 cells shows that Gelam honey exerts protective effects against diabetes- and hyperglycemia-induced oxidative stress by improving insulin content and insulin resistance. |
| format | Article |
| id | doaj-art-48f53938ff2d42dea418443ca76ad77d |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-48f53938ff2d42dea418443ca76ad77d2025-02-03T06:42:26ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/367312367312Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster CellsKalaivani Batumalaie0Sher Zaman Safi1Kamaruddin Mohd Yusof2Ikram Shah Ismail3Shamala Devi Sekaran4Rajes Qvist5Department of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Molecular Biology and Genetics, Faculty of Arts and Science, Canik Basari University, Samsun, TurkeyDepartment of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, MalaysiaBackground. Oxidative stress induced by reactive oxygen and nitrogen species is critically involved in the impairment of β-cell function during the development of diabetes. Methods. HIT-T15 cells were cultured in 5% CO2 and then preincubated with Gelam honey extracts (20, 40, 60, and 80 µg/mL) as well as quercetin (20, 40, 60, and 80 µM), prior to stimulation by 20 and 50 mM of glucose. Cell lysate was collected to determine the effect of honey extracts and quercetin on the stress activated NF-κB, MAPK pathways, and the Akt (ser473) activated insulin signaling pathway. Results. HIT-T15 cells cultured under hyperglycemic conditions demonstrated insulin resistance with a significant increase in the levels of MAPK, NF-κB, and IRS-1 serine phosphorylation (ser307); however, Akt expression and insulin contents are significantly decreased. Pretreatment with quercetin and Gelam honey extract improved insulin resistance and insulin content by reducing the expression of MAPK, NF-κB, and IRS-1 serine phosphorylation (ser307) and increasing the expression of Akt significantly. Conclusion. Gelam honey-induced differential expression of MAPK, NF-κB, IRS-1 (ser307), and Akt in HIT-T15 cells shows that Gelam honey exerts protective effects against diabetes- and hyperglycemia-induced oxidative stress by improving insulin content and insulin resistance.http://dx.doi.org/10.1155/2013/367312 |
| spellingShingle | Kalaivani Batumalaie Sher Zaman Safi Kamaruddin Mohd Yusof Ikram Shah Ismail Shamala Devi Sekaran Rajes Qvist Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells International Journal of Endocrinology |
| title | Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells |
| title_full | Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells |
| title_fullStr | Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells |
| title_full_unstemmed | Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells |
| title_short | Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells |
| title_sort | effect of gelam honey on the oxidative stress induced signaling pathways in pancreatic hamster cells |
| url | http://dx.doi.org/10.1155/2013/367312 |
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