DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS

Objective: To study the informative value of the detection of mycobacteria in blood with the cultural method in patients with suspected tuberculous sepsis and to determine the most significant clinical and laboratory criteria for testing. Materials and methods: The investigation to detect M.tubercul...

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Main Authors: V. N. Zimina, O. E. Mikova, T. A. Varetskaya, D. A. Oborin, A. G. Zemskov, S. Yu. Degtyareva
Format: Article
Language:Russian
Published: Journal Infectology 2017-04-01
Series:Журнал инфектологии
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Online Access:https://journal.niidi.ru/jofin/article/view/563
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author V. N. Zimina
O. E. Mikova
T. A. Varetskaya
D. A. Oborin
A. G. Zemskov
S. Yu. Degtyareva
author_facet V. N. Zimina
O. E. Mikova
T. A. Varetskaya
D. A. Oborin
A. G. Zemskov
S. Yu. Degtyareva
author_sort V. N. Zimina
collection DOAJ
description Objective: To study the informative value of the detection of mycobacteria in blood with the cultural method in patients with suspected tuberculous sepsis and to determine the most significant clinical and laboratory criteria for testing. Materials and methods: The investigation to detect M.tuberculosis was fulfilled in 159 HIV-positive patients with suspected tuberculosis sepsis. Blood culture was completed with culture medium Myco/F Lytic Culture Vials and analyzer BACTEC 9050. Results: Mycobacteria were detected in blood of 19 patients (11,9% of all patients): in 18 patients the growth of М. tuberculosis complex was detected (25,3% of all patients with diagnosed tuberculosis) and in 1 patient it was Mycobacterium avium complex (0,6% of all patients). It was shown, that the probability of M.tuberculosis detection was especially associated with the severity of the disease, immunosupression (less than 100 cells/mkl), hemoglobin quantity less than 90 g/l (levels were determined through the seeking for the most significant cutoffs). It was not proofed, that meningoencephalitis develops more often in patients with proven bacteremia. There were no evident differences in detection frequency of mycobacteria in sputum between patients with tuberculous sepsis and without it.
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issn 2072-6732
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spelling doaj-art-48eac2a54b43441d83ec71a1567199a12025-08-20T02:55:14ZrusJournal InfectologyЖурнал инфектологии2072-67322017-04-0191626610.22625/2072-6732-2017-9-1-62-66533DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTSV. N. Zimina0O. E. Mikova1T. A. Varetskaya2D. A. Oborin3A. G. Zemskov4S. Yu. Degtyareva5Russian University of Peoples’ FrendshipPerm Regional Center for Prevention and Control of AIDS and infectious diseasesPerm Regional Center for Prevention and Control of AIDS and infectious diseasesPerm Regional Center for Prevention and Control of AIDS and infectious diseasesPerm Regional Clinical Infection HospitalRussian University of Peoples’ FrendshipObjective: To study the informative value of the detection of mycobacteria in blood with the cultural method in patients with suspected tuberculous sepsis and to determine the most significant clinical and laboratory criteria for testing. Materials and methods: The investigation to detect M.tuberculosis was fulfilled in 159 HIV-positive patients with suspected tuberculosis sepsis. Blood culture was completed with culture medium Myco/F Lytic Culture Vials and analyzer BACTEC 9050. Results: Mycobacteria were detected in blood of 19 patients (11,9% of all patients): in 18 patients the growth of М. tuberculosis complex was detected (25,3% of all patients with diagnosed tuberculosis) and in 1 patient it was Mycobacterium avium complex (0,6% of all patients). It was shown, that the probability of M.tuberculosis detection was especially associated with the severity of the disease, immunosupression (less than 100 cells/mkl), hemoglobin quantity less than 90 g/l (levels were determined through the seeking for the most significant cutoffs). It was not proofed, that meningoencephalitis develops more often in patients with proven bacteremia. There were no evident differences in detection frequency of mycobacteria in sputum between patients with tuberculous sepsis and without it.https://journal.niidi.ru/jofin/article/view/563hiv-infectiontuberculosismycobacterial bacteremiatuberculous sepsismbt-bacteremiablood culturebloodstream infection.
spellingShingle V. N. Zimina
O. E. Mikova
T. A. Varetskaya
D. A. Oborin
A. G. Zemskov
S. Yu. Degtyareva
DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
Журнал инфектологии
hiv-infection
tuberculosis
mycobacterial bacteremia
tuberculous sepsis
mbt-bacteremia
blood culture
bloodstream infection.
title DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
title_full DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
title_fullStr DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
title_full_unstemmed DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
title_short DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN BLOOD FOR DIAGNOSIS OF GENERALISED TUBERCULOSIS IN HIV-POSITIVE PATIENTS
title_sort detection of mycobacterium tuberculosis in blood for diagnosis of generalised tuberculosis in hiv positive patients
topic hiv-infection
tuberculosis
mycobacterial bacteremia
tuberculous sepsis
mbt-bacteremia
blood culture
bloodstream infection.
url https://journal.niidi.ru/jofin/article/view/563
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