circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8
High circ_0020123 expression is associated with poor prognosis in patients with non-small cell lung cancer (NSCLC) as previously reported. Whether circ_0020123 also plays an oncogenic role in lung adenocarcinoma (LUAD) is still unknown. Additionally, circ_0020123 is derived from part of exon (1312–3...
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De Gruyter
2022-03-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2022-0434 |
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| author | Wei Wei Wang Changyong Wang Lele Zhang Jianfeng |
| author_facet | Wei Wei Wang Changyong Wang Lele Zhang Jianfeng |
| author_sort | Wei Wei |
| collection | DOAJ |
| description | High circ_0020123 expression is associated with poor prognosis in patients with non-small cell lung cancer (NSCLC) as previously reported. Whether circ_0020123 also plays an oncogenic role in lung adenocarcinoma (LUAD) is still unknown. Additionally, circ_0020123 is derived from part of exon (1312–3851) from its host gene PDZ domain-containing protein 8 (PDZD8). We hypothesized that circ_0020123 might affect malignant behaviors of LUAD cells by regulating PDZD8. Reverse transcription quantitative polymerase chain reaction revealed that PDZD8 was highly expressed in LUAD tissues and cells. PDZD8 knockdown suppressed LUAD cell proliferation and migration as shown by colony formation assays, Ethynyl deoxyuridine incorporation assays, Transwell assays, and wound healing assays. circ_0020123 was also found to be upregulated in LUAD tissues and cells. Moreover, circ_0020123 positively regulated PDZD8 expression in LUAD cells but exerted no significant effect on the transcriptional level of PDZD8. Mechanistically, circ_0020123 act as a competing endogenous RNA (ceRNA) to interact with miR-1283, thereby releasing the repression on PDZD8. Moreover, PDZD8 overexpression rescued the suppressive effect of circ_0020123 knockdown on LUAD cell proliferation and migration. In conclusion, circ_0020123 interacts with miR-1283 as a ceRNA to regulate PDZD8 expression, thus promoting the proliferation and migration of LUAD cells. The study might provide new biomarkers for future LUAD investigation. |
| format | Article |
| id | doaj-art-48e853454c9249d2b771ea253801322e |
| institution | DOAJ |
| issn | 2391-5463 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | De Gruyter |
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| spelling | doaj-art-48e853454c9249d2b771ea253801322e2025-08-20T03:06:37ZengDe GruyterOpen Medicine2391-54632022-03-0117153654910.1515/med-2022-0434circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8Wei Wei0Wang Changyong1Wang Lele2Zhang Jianfeng3Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu, ChinaDepartment of Cardiothoracic Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu, ChinaDepartment of Cardiothoracic Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu, ChinaDepartment of Cardiothoracic Surgery, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, Jiangsu, ChinaHigh circ_0020123 expression is associated with poor prognosis in patients with non-small cell lung cancer (NSCLC) as previously reported. Whether circ_0020123 also plays an oncogenic role in lung adenocarcinoma (LUAD) is still unknown. Additionally, circ_0020123 is derived from part of exon (1312–3851) from its host gene PDZ domain-containing protein 8 (PDZD8). We hypothesized that circ_0020123 might affect malignant behaviors of LUAD cells by regulating PDZD8. Reverse transcription quantitative polymerase chain reaction revealed that PDZD8 was highly expressed in LUAD tissues and cells. PDZD8 knockdown suppressed LUAD cell proliferation and migration as shown by colony formation assays, Ethynyl deoxyuridine incorporation assays, Transwell assays, and wound healing assays. circ_0020123 was also found to be upregulated in LUAD tissues and cells. Moreover, circ_0020123 positively regulated PDZD8 expression in LUAD cells but exerted no significant effect on the transcriptional level of PDZD8. Mechanistically, circ_0020123 act as a competing endogenous RNA (ceRNA) to interact with miR-1283, thereby releasing the repression on PDZD8. Moreover, PDZD8 overexpression rescued the suppressive effect of circ_0020123 knockdown on LUAD cell proliferation and migration. In conclusion, circ_0020123 interacts with miR-1283 as a ceRNA to regulate PDZD8 expression, thus promoting the proliferation and migration of LUAD cells. The study might provide new biomarkers for future LUAD investigation.https://doi.org/10.1515/med-2022-0434lung adenocarcinomapdzd8circ_0020123mir-1283cerna |
| spellingShingle | Wei Wei Wang Changyong Wang Lele Zhang Jianfeng circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 Open Medicine lung adenocarcinoma pdzd8 circ_0020123 mir-1283 cerna |
| title | circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 |
| title_full | circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 |
| title_fullStr | circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 |
| title_full_unstemmed | circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 |
| title_short | circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8 |
| title_sort | circ 0020123 promotes cell proliferation and migration in lung adenocarcinoma via pdzd8 |
| topic | lung adenocarcinoma pdzd8 circ_0020123 mir-1283 cerna |
| url | https://doi.org/10.1515/med-2022-0434 |
| work_keys_str_mv | AT weiwei circ0020123promotescellproliferationandmigrationinlungadenocarcinomaviapdzd8 AT wangchangyong circ0020123promotescellproliferationandmigrationinlungadenocarcinomaviapdzd8 AT wanglele circ0020123promotescellproliferationandmigrationinlungadenocarcinomaviapdzd8 AT zhangjianfeng circ0020123promotescellproliferationandmigrationinlungadenocarcinomaviapdzd8 |