Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis
Reverse Phase High Performance Liquid Chromatography (RP-HPLC) is an efficient and reliable technique employed in pharmaceutical and forensic analysis. The assay determination in pharmaceutical and certain molecular detection is performed using this technique. Performance of chromatographic columns...
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| Format: | Article |
| Language: | Arabic |
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Naif University Publishing House
2024-07-01
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| Series: | Arab Journal of Forensic Sciences & Forensic Medicine |
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| Online Access: | https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2987 |
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| author | Khalid Sajjad Feras Tahir Jamshaid Majed Musalim Albluwi Umair Ikram Dar Abid Hussain |
| author_facet | Khalid Sajjad Feras Tahir Jamshaid Majed Musalim Albluwi Umair Ikram Dar Abid Hussain |
| author_sort | Khalid Sajjad Feras |
| collection | DOAJ |
| description | Reverse Phase High Performance Liquid Chromatography (RP-HPLC) is an efficient and reliable technique employed in pharmaceutical and forensic analysis. The assay determination in pharmaceutical and certain molecular detection is performed using this technique. Performance of chromatographic columns play a central role in development of more efficient separation methods. It is one of the main component of system suitability testing in a chromatographic system. The column efficiency can be assessed by using various traditional methods and reproducible results can be evaluated using liquid chromatography We studied the column efficiency using RP-HPLC technique for pharmaceutical drug product Aceclofenac in a validated method. Two mobile phases combinations Acetonitrile: Methanol (80:20 v/v) and Acetonitrile: Methanol: Ammonia (225:50:1 v/v) were compared on C-8 Lichrospher RP-HPLC column for Aceclofenac and three tablet samples (30 ppm). The height equivalent to theoretical plate (HETP) referred as direct measure of column efficiency produced efficient results (530-545 and 190-230) for respective mobile phases. The lower HEPT is an indication of efficient elution in second mobile phase. The change in polarity as per pKa of the analyte may enhance elution and resolution. helpful in method development for pharmaceutical and forensic drug analysis using liquid chromatography. |
| format | Article |
| id | doaj-art-48d4002a253d41c59ba19f3d15a8a265 |
| institution | Kabale University |
| issn | 1658-6786 1658-6794 |
| language | Arabic |
| publishDate | 2024-07-01 |
| publisher | Naif University Publishing House |
| record_format | Article |
| series | Arab Journal of Forensic Sciences & Forensic Medicine |
| spelling | doaj-art-48d4002a253d41c59ba19f3d15a8a2652025-08-20T03:44:32ZaraNaif University Publishing HouseArab Journal of Forensic Sciences & Forensic Medicine1658-67861658-67942024-07-016Special Issue14515410.26735/IKRK25292691Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug AnalysisKhalid Sajjad Feras0Tahir Jamshaid1Majed Musalim Albluwi2Umair Ikram Dar3Abid Hussain4College of Criminal Justice, Department of Forensic Sciences, Naif Arab University for Security Sciences, Riyadh, Saudi ArabiaLahore College of Pharmaceutical Sciences, 18km Lahore-Raiwind Road, Raiwind, Lahore, PakistanDepartment of Forensic Chemistry, General Administration of Criminal Evidences, Public Security, Riyadh, Saudi ArabiaLahore College of Pharmaceutical Sciences, 18km Lahore-Raiwind Road, Raiwind, Lahore, PakistanPunjab Forensic Science Agency, Lahore, Punjab, PakistanReverse Phase High Performance Liquid Chromatography (RP-HPLC) is an efficient and reliable technique employed in pharmaceutical and forensic analysis. The assay determination in pharmaceutical and certain molecular detection is performed using this technique. Performance of chromatographic columns play a central role in development of more efficient separation methods. It is one of the main component of system suitability testing in a chromatographic system. The column efficiency can be assessed by using various traditional methods and reproducible results can be evaluated using liquid chromatography We studied the column efficiency using RP-HPLC technique for pharmaceutical drug product Aceclofenac in a validated method. Two mobile phases combinations Acetonitrile: Methanol (80:20 v/v) and Acetonitrile: Methanol: Ammonia (225:50:1 v/v) were compared on C-8 Lichrospher RP-HPLC column for Aceclofenac and three tablet samples (30 ppm). The height equivalent to theoretical plate (HETP) referred as direct measure of column efficiency produced efficient results (530-545 and 190-230) for respective mobile phases. The lower HEPT is an indication of efficient elution in second mobile phase. The change in polarity as per pKa of the analyte may enhance elution and resolution. helpful in method development for pharmaceutical and forensic drug analysis using liquid chromatography.https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2987forensic sciencesreversed-phase highperformance liquid chromatography (rp-hplc)system suitability testingaceclofenac (ace) |
| spellingShingle | Khalid Sajjad Feras Tahir Jamshaid Majed Musalim Albluwi Umair Ikram Dar Abid Hussain Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis Arab Journal of Forensic Sciences & Forensic Medicine forensic sciences reversed-phase highperformance liquid chromatography (rp-hplc) system suitability testing aceclofenac (ace) |
| title | Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis |
| title_full | Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis |
| title_fullStr | Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis |
| title_full_unstemmed | Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis |
| title_short | Optimization and Evaluation of Column Efficiency in RP-HPLC Method for Pharmaceutical and Forensic Drug Analysis |
| title_sort | optimization and evaluation of column efficiency in rp hplc method for pharmaceutical and forensic drug analysis |
| topic | forensic sciences reversed-phase highperformance liquid chromatography (rp-hplc) system suitability testing aceclofenac (ace) |
| url | https://journals.nauss.edu.sa/index.php/AJFSFM/article/view/2987 |
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