MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/2320640 |
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| author | Feng Huang Junsong Zhang Diyuan Yang Yuelan Zhang Jinxiang Huang Yaochang Yuan Xuefeng Li Gen Lu |
| author_facet | Feng Huang Junsong Zhang Diyuan Yang Yuelan Zhang Jinxiang Huang Yaochang Yuan Xuefeng Li Gen Lu |
| author_sort | Feng Huang |
| collection | DOAJ |
| description | Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing. Expression profiles of whole blood microRNAs were altered and distinctly different in adenovirus-infected children. The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. Potential host target genes were predicated and validated by qRT-PCR to study the impact of microRNAs on the host genes. Most of the target genes were involved in the MAPK signaling pathway and innate immune response. These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia. |
| format | Article |
| id | doaj-art-48cffbd536984cfeb3dcdc51eef0c689 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-48cffbd536984cfeb3dcdc51eef0c6892025-08-20T02:05:55ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/23206402320640MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia ChildrenFeng Huang0Junsong Zhang1Diyuan Yang2Yuelan Zhang3Jinxiang Huang4Yaochang Yuan5Xuefeng Li6Gen Lu7Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510120 Guangdong, ChinaInstitute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080 Guangdong, ChinaGuangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510120 Guangdong, ChinaThe Second Affiliated Hospital of Guangzhou Medical University, the State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s hospital, Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436 Guangdong, ChinaThe Second Affiliated Hospital of Guangzhou Medical University, the State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s hospital, Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436 Guangdong, ChinaInstitute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080 Guangdong, ChinaThe Second Affiliated Hospital of Guangzhou Medical University, the State Key Laboratory of Respiratory Disease, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s hospital, Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436 Guangdong, ChinaGuangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510120 Guangdong, ChinaHuman adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing. Expression profiles of whole blood microRNAs were altered and distinctly different in adenovirus-infected children. The top 3 upregulated miRNA (hsa-miR-127-3p, hsa-miR-493-5p, and hsa-miR-409-3p) were identified in adenovirus-infected children and provided a clear distinction between infected and healthy individuals. Potential host target genes were predicated and validated by qRT-PCR to study the impact of microRNAs on the host genes. Most of the target genes were involved in the MAPK signaling pathway and innate immune response. These highly upregulated microRNAs may have crucial roles in Adv pathogenesis and are potential biomarkers for adenovirus-infected pneumonia.http://dx.doi.org/10.1155/2018/2320640 |
| spellingShingle | Feng Huang Junsong Zhang Diyuan Yang Yuelan Zhang Jinxiang Huang Yaochang Yuan Xuefeng Li Gen Lu MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children Mediators of Inflammation |
| title | MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children |
| title_full | MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children |
| title_fullStr | MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children |
| title_full_unstemmed | MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children |
| title_short | MicroRNA Expression Profile of Whole Blood Is Altered in Adenovirus-Infected Pneumonia Children |
| title_sort | microrna expression profile of whole blood is altered in adenovirus infected pneumonia children |
| url | http://dx.doi.org/10.1155/2018/2320640 |
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