Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei

The fermentation condition for efficacious production of β-glucanase and xylanase by Trichoderma reesei was studied. The results showed that the best carbon and nitrogen sources for xylanase production were lactose combined with beef extract. On the other hand, for β-glucanase production combination...

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Main Authors: GU Sai-hong, SUN Jian-yi, LI Wei-fen, XU Zi-rong
Format: Article
Language:English
Published: Zhejiang University Press 2003-09-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/1008-9209.2003.05.0545
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author GU Sai-hong
SUN Jian-yi
LI Wei-fen
XU Zi-rong
author_facet GU Sai-hong
SUN Jian-yi
LI Wei-fen
XU Zi-rong
author_sort GU Sai-hong
collection DOAJ
description The fermentation condition for efficacious production of β-glucanase and xylanase by Trichoderma reesei was studied. The results showed that the best carbon and nitrogen sources for xylanase production were lactose combined with beef extract. On the other hand, for β-glucanase production combination of wheat bran and ammonium sulphate was most successful. The optimal initial pH and fermentation condition was 4.0 at 30℃ for xylanase production and 5.0 at 30℃ for β-glucanase production. In addition, Toween 20, Toween 80 and betaine were found to increase yields of xylanase and β-glucanase, the optimal stimulator being betaine for xylanase production and Toween 20 for β-glucanase production.. Based on course of β-glucanase and xylanase by T. reesei, the initiation of xylanase and β-glucanase production were at 20 h and 24 h, respectively, while the peak time for their production were at 48h and 44h, respectively.
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2097-5155
language English
publishDate 2003-09-01
publisher Zhejiang University Press
record_format Article
series 浙江大学学报. 农业与生命科学版
spelling doaj-art-48cd6703c4c74dccbe285f8e0f6cf2b42025-08-20T03:58:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-09-012954554910.3785/1008-9209.2003.05.054510089209Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reeseiGU Sai-hongSUN Jian-yiLI Wei-fenXU Zi-rongThe fermentation condition for efficacious production of β-glucanase and xylanase by Trichoderma reesei was studied. The results showed that the best carbon and nitrogen sources for xylanase production were lactose combined with beef extract. On the other hand, for β-glucanase production combination of wheat bran and ammonium sulphate was most successful. The optimal initial pH and fermentation condition was 4.0 at 30℃ for xylanase production and 5.0 at 30℃ for β-glucanase production. In addition, Toween 20, Toween 80 and betaine were found to increase yields of xylanase and β-glucanase, the optimal stimulator being betaine for xylanase production and Toween 20 for β-glucanase production.. Based on course of β-glucanase and xylanase by T. reesei, the initiation of xylanase and β-glucanase production were at 20 h and 24 h, respectively, while the peak time for their production were at 48h and 44h, respectively.https://www.academax.com/doi/10.3785/1008-9209.2003.05.0545<italic>Trichoderma reesei</italic>xylanase<italic>β</italic>-glucanasefermentation condition
spellingShingle GU Sai-hong
SUN Jian-yi
LI Wei-fen
XU Zi-rong
Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
浙江大学学报. 农业与生命科学版
<italic>Trichoderma reesei</italic>
xylanase
<italic>β</italic>-glucanase
fermentation condition
title Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
title_full Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
title_fullStr Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
title_full_unstemmed Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
title_short Effects of fermentation condition on production of β-glucanase and xylanase by Trichoderma reesei
title_sort effects of fermentation condition on production of β glucanase and xylanase by trichoderma reesei
topic <italic>Trichoderma reesei</italic>
xylanase
<italic>β</italic>-glucanase
fermentation condition
url https://www.academax.com/doi/10.3785/1008-9209.2003.05.0545
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AT sunjianyi effectsoffermentationconditiononproductionofbglucanaseandxylanasebytrichodermareesei
AT liweifen effectsoffermentationconditiononproductionofbglucanaseandxylanasebytrichodermareesei
AT xuzirong effectsoffermentationconditiononproductionofbglucanaseandxylanasebytrichodermareesei