Study on antivirus activity of crude extract from eighteen kinds of traditional Chinese medicine against duck plague virus in vitro
Traditional Chinese medicine is rich in resources and has the characteristics of low prices and low-toxic side effects, which is widely used in the treatment of animal diseases, including viral diseases. Duck plague virus (DPV) is an acute, contagious herpesvirus infection of duck, geese, and swans...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2013-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.082 |
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| Summary: | Traditional Chinese medicine is rich in resources and has the characteristics of low prices and low-toxic side effects, which is widely used in the treatment of animal diseases, including viral diseases. Duck plague virus (DPV) is an acute, contagious herpesvirus infection of duck, geese, and swans of all ages and species. This disease has been responsible for significant economic losses in domestic and wild waterfowl as results of mortality, and decreased egg production. There is no effective drug on the therapy of this virus disease. We attempted to screen out a few of effective traditional Chinese medicines on DPV from eighteen kinds of traditional Chinese medicines. The methods were as follows.At first, the cytotoxic effect of the water extracts and alcohol extracts of the traditional Chinese medicines on DEFs monolayer were determined by the method of cytopathic effect (CPE). All the concentrations of extracts used in antiviral activity assays were below the maximal non-cytotoxic concentration (MNCC). In the antiviral activity assay, the method of CPE was used in preliminary screening for the effective extracts. The virus inhibitory ratios of the effective extracts against DPV were tested by the method of [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, MTT] colorimetric analysis.The results showed that the virus inhibitory ratios against DPV were 76.50% and 48.91% with the concentrations 0.062 5 mg/mL and 0.007 8 mg/mL of Rhizoma polygoni cuspidati and Radix rubiae. Additionally, the 50% inhibitory concentration of Rhizoma polygoni cuspidati and Radix rubiae were 0.022 7 mg/mL and greater than 0.007 8 mg/mL respectively.The result above indicated that the alcohol extracts of Rhizoma polygoni cuspidati and Radix rubiae showed the antiviral activities at varying degrees in vitro and the Rhizoma polygoni cuspidati, whose antiviral activity is better than Radix rubiae, might be a potential agent in the prevention of DPV infections. |
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| ISSN: | 1008-9209 2097-5155 |