Advances and future trends in real-time precision optical control of chemical processes in live cells

Abstract Traditional chemical interventions regulate cellular processes but often affect non-target biomolecules. Precise and site-specific control is crucial for studying complex systems. Conventional laser-based methods offer high spatial precision and speed but rely on prior sample knowledge and...

Full description

Saved in:
Bibliographic Details
Main Authors: Chi Zhang, Bin Dong, Shivam Mahapatra, Seohee Ma
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:npj Imaging
Online Access:https://doi.org/10.1038/s44303-025-00083-1
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Traditional chemical interventions regulate cellular processes but often affect non-target biomolecules. Precise and site-specific control is crucial for studying complex systems. Conventional laser-based methods offer high spatial precision and speed but rely on prior sample knowledge and do not apply to highly mobile targets. Real-time precision opto-control (RPOC) overcomes these limits using closed-loop feedback for automated and signal-determined real-time laser activation to regulate chemical processes in live biological samples. This review compares RPOC with other optical control techniques and explores its advancements, applications, and future directions.
ISSN:2948-197X