Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan
Background. Persistent Helicobacter pylori infection may induce several upper gastrointestinal diseases. Two major virulence factors of H. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distrib...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/7521913 |
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| author | Chih-Ho Lai Ju-Chun Huang Chuan Chiang-Ni Ju-Pi Li Lii-Tzu Wu Hua-Shan Wu Yu-Chen Sun Mei-Ling Lin Ju-Fang Lee Hwai-Jeng Lin |
| author_facet | Chih-Ho Lai Ju-Chun Huang Chuan Chiang-Ni Ju-Pi Li Lii-Tzu Wu Hua-Shan Wu Yu-Chen Sun Mei-Ling Lin Ju-Fang Lee Hwai-Jeng Lin |
| author_sort | Chih-Ho Lai |
| collection | DOAJ |
| description | Background. Persistent Helicobacter pylori infection may induce several upper gastrointestinal diseases. Two major virulence factors of H. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution of vacA and cag-pathogenicity island (cag-PAI) alleles varies in H. pylori isolated from patients in different geographic regions. Aim. To assess the association between mixed infection of H. pylori clinical isolates from Taiwanese patients and the severity of gastrointestinal diseases. Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes. Results. The prevalence of mixed infections of all H. pylori-infected patients was 28.6% (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6%) was much higher than that with other gastrointestinal diseases (P<0.05). Conclusions. H. pylori mixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development. |
| format | Article |
| id | doaj-art-48aaf590f358491fb03e8414a457b2f0 |
| institution | OA Journals |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-48aaf590f358491fb03e8414a457b2f02025-08-20T02:37:59ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/75219137521913Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in TaiwanChih-Ho Lai0Ju-Chun Huang1Chuan Chiang-Ni2Ju-Pi Li3Lii-Tzu Wu4Hua-Shan Wu5Yu-Chen Sun6Mei-Ling Lin7Ju-Fang Lee8Hwai-Jeng Lin9Graduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, TaiwanGraduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, TaiwanGraduate Institute of Basic Medical Science, School of Medicine, China Medical University, Taichung, TaiwanDepartment of Medical Research and Department of Laboratory Medicine, China Medical University and Hospital, Taichung, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan, TaiwanGraduate Institute of Biomedical Sciences, Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan, TaiwanDivision of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanBackground. Persistent Helicobacter pylori infection may induce several upper gastrointestinal diseases. Two major virulence factors of H. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution of vacA and cag-pathogenicity island (cag-PAI) alleles varies in H. pylori isolated from patients in different geographic regions. Aim. To assess the association between mixed infection of H. pylori clinical isolates from Taiwanese patients and the severity of gastrointestinal diseases. Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes. Results. The prevalence of mixed infections of all H. pylori-infected patients was 28.6% (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6%) was much higher than that with other gastrointestinal diseases (P<0.05). Conclusions. H. pylori mixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development.http://dx.doi.org/10.1155/2016/7521913 |
| spellingShingle | Chih-Ho Lai Ju-Chun Huang Chuan Chiang-Ni Ju-Pi Li Lii-Tzu Wu Hua-Shan Wu Yu-Chen Sun Mei-Ling Lin Ju-Fang Lee Hwai-Jeng Lin Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan Gastroenterology Research and Practice |
| title | Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan |
| title_full | Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan |
| title_fullStr | Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan |
| title_full_unstemmed | Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan |
| title_short | Mixed Infections of Helicobacter pylori Isolated from Patients with Gastrointestinal Diseases in Taiwan |
| title_sort | mixed infections of helicobacter pylori isolated from patients with gastrointestinal diseases in taiwan |
| url | http://dx.doi.org/10.1155/2016/7521913 |
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