Protocol for the establishment of murine orthotopic lung tumors and the assessment of contralateral pulmonal metastasis by flow cytometry

Protocol for the establishment of murine orthotopic lung tumors and the assessment of contralateral pulmonal metastasis by flow cytometry

Summary: Murine orthotopic tumor models can accurately resemble cancer biology characteristics including metastasis, drug sensitivity, and remodeling of the tumor microenvironment. Here, we present a protocol for establishing murine orthotopic lung tumors and assessing contralateral pulmonary metast...

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Bibliographic Details
Main Authors: Gregor Zaun, Siyang Liu, Maximilian Webendörfer, Abubakar Wani, Diego Rodriguez, Douglas R. Green, Halime Kalkavan
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Single Cell
Flow Cytometry
Cancer
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001996
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