Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney
Considering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopa...
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2016-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2016/6302376 |
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| author | N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi |
| author_facet | N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi |
| author_sort | N. L. Rukavina Mikusic |
| collection | DOAJ |
| description | Considering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopamine uptake in renal tissue or Na+, K+-ATPase activity; meanwhile, Ang-(1-7) was able to increase 3H-dopamine uptake and decreased Na+, K+-ATPase activity in renal cortex. Ang-(1-7) and dopamine together decreased further Na+, K+-ATPase activity showing an additive effect on the sodium pump. In addition, hydrocortisone reversed Ang-(1-7)-dopamine overinhibition on the enzyme, suggesting that this inhibition is closely related to Ang-(1-7) stimulation on renal dopamine uptake. Both anantin and cANP (4-23-amide) did not modify CNP effects on 3H-dopamine uptake by tubular cells. The Mas receptor antagonist, A-779, blocked the increase elicited by Ang-(1-7) on 3H-dopamine uptake. The stimulatory uptake induced by Ang-(1-7) was even more pronounced in the presence of losartan, suggesting an inhibitory effect of Ang-(1-7) on AT1 receptors on 3H-dopamine uptake. By increasing dopamine bioavailability in tubular cells, Ang-(1-7) enhances Na+, K+-ATPase activity inhibition, contributing to its natriuretic and diuretic effects. |
| format | Article |
| id | doaj-art-48970334847541f891ffd0d982de9988 |
| institution | Kabale University |
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| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Scientifica |
| spelling | doaj-art-48970334847541f891ffd0d982de99882025-02-03T05:54:02ZengWileyScientifica2090-908X2016-01-01201610.1155/2016/63023766302376Regulation of Dopamine Uptake by Vasoactive Peptides in the KidneyN. L. Rukavina Mikusic0N. M. Kouyoumdzian1E. Rouvier2M. M. Gironacci3J. E. Toblli4B. E. Fernández5M. R. Choi6Instituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaCátedras de Química Biológica, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaInstituto de Investigaciones Cardiológicas ININCA, UBA-CONICET, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, ArgentinaConsidering the key role of renal dopamine in tubular sodium handling, we hypothesized that c-type natriuretic peptide (CNP) and Ang-(1-7) may regulate renal dopamine availability in tubular cells, contributing to Na+, K+-ATPase inhibition. Present results show that CNP did not affect either 3H-dopamine uptake in renal tissue or Na+, K+-ATPase activity; meanwhile, Ang-(1-7) was able to increase 3H-dopamine uptake and decreased Na+, K+-ATPase activity in renal cortex. Ang-(1-7) and dopamine together decreased further Na+, K+-ATPase activity showing an additive effect on the sodium pump. In addition, hydrocortisone reversed Ang-(1-7)-dopamine overinhibition on the enzyme, suggesting that this inhibition is closely related to Ang-(1-7) stimulation on renal dopamine uptake. Both anantin and cANP (4-23-amide) did not modify CNP effects on 3H-dopamine uptake by tubular cells. The Mas receptor antagonist, A-779, blocked the increase elicited by Ang-(1-7) on 3H-dopamine uptake. The stimulatory uptake induced by Ang-(1-7) was even more pronounced in the presence of losartan, suggesting an inhibitory effect of Ang-(1-7) on AT1 receptors on 3H-dopamine uptake. By increasing dopamine bioavailability in tubular cells, Ang-(1-7) enhances Na+, K+-ATPase activity inhibition, contributing to its natriuretic and diuretic effects.http://dx.doi.org/10.1155/2016/6302376 |
| spellingShingle | N. L. Rukavina Mikusic N. M. Kouyoumdzian E. Rouvier M. M. Gironacci J. E. Toblli B. E. Fernández M. R. Choi Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney Scientifica |
| title | Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
| title_full | Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
| title_fullStr | Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
| title_full_unstemmed | Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
| title_short | Regulation of Dopamine Uptake by Vasoactive Peptides in the Kidney |
| title_sort | regulation of dopamine uptake by vasoactive peptides in the kidney |
| url | http://dx.doi.org/10.1155/2016/6302376 |
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