Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss)
Nanoparticles of copper oxide, released uncontrollably into the environment, pose a threat to living organisms. One of the mechanisms of their toxicity is the induction of oxidative stress, contributing to the disruption of antioxidant mechanisms. The aim of the present study was to assess the degre...
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Taylor & Francis Group
2024-07-01
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| Series: | The European Zoological Journal |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/24750263.2024.2385820 |
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| author | A. Sielska M. Kowalska-Góralska I. Szućko-Kociuba L. Skuza |
| author_facet | A. Sielska M. Kowalska-Góralska I. Szućko-Kociuba L. Skuza |
| author_sort | A. Sielska |
| collection | DOAJ |
| description | Nanoparticles of copper oxide, released uncontrollably into the environment, pose a threat to living organisms. One of the mechanisms of their toxicity is the induction of oxidative stress, contributing to the disruption of antioxidant mechanisms. The aim of the present study was to assess the degree and toxic dose of copper oxide nanoparticle (CuO-NPs) associated with the induction of oxidative stress and compare them to the toxicity of copper sulfate (VI) (CuSO4) by measuring the expression of genes encoding some key enzymes involved in protection against oxidative stress: glutathione peroxidase (gpx), cytochrome P450 (cyp1a), heat shock protein (hsp70) and superoxide dismutase (sod). Hatchlings of rainbow trout (Oncorhynchus mykiss) were used as biological material. The test individuals were exposed for 2 hours to CuO-NPs and CuSO4 in the concentration range of 4–256 mg dm−3. Individuals exposed to all concentrations of CuO-NPs showed an increase in the expression of gpx, cyp1a and hsp70 genes, and a decrease in sod gene expression, while the hatchlings exposed to CuSO4 exhibited an increase in the expression of all genes. The expression of hsp70 and sod genes in the hatchlings exposed to CuSO4 was higher than in the CuO-NP treatment, while the expression levels of gpx and cyp1a were similar. Based on the obtained results, potentially higher toxicity of CuSO4 was observed in the hatchlings of Oncorhynchus mykiss. However, the elevated expression of gpx, cyp1a and hsp70 after the exposure to CuO-NPs, indicates toxicity via oxidative stress. The decrease (sod in hatchlings exposed to CuO-NPs) or increase of gene expression was not dose-dependent. The presented study confirms a need for further research regarding the toxicity of metal nanoparticles to aquatic organisms and the necessity for monitoring the release of CuO-NPs into the aquatic environment. |
| format | Article |
| id | doaj-art-4889adf68f7847b494a87f26da1cc9d3 |
| institution | OA Journals |
| issn | 2475-0263 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | The European Zoological Journal |
| spelling | doaj-art-4889adf68f7847b494a87f26da1cc9d32025-08-20T02:31:29ZengTaylor & Francis GroupThe European Zoological Journal2475-02632024-07-0191289790510.1080/24750263.2024.2385820Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss)A. Sielska0M. Kowalska-Góralska1I. Szućko-Kociuba2L. Skuza3Institute of Biology, University of Szczecin, Szczecin, PolandDepartment of Hydrobiology and Aquaculture, Faculty of Biology and Animal Science, Wrocław University of Environmental and Life Sciences, Wroclaw, PolandInstitute of Biology, University of Szczecin, Szczecin, PolandInstitute of Biology, University of Szczecin, Szczecin, PolandNanoparticles of copper oxide, released uncontrollably into the environment, pose a threat to living organisms. One of the mechanisms of their toxicity is the induction of oxidative stress, contributing to the disruption of antioxidant mechanisms. The aim of the present study was to assess the degree and toxic dose of copper oxide nanoparticle (CuO-NPs) associated with the induction of oxidative stress and compare them to the toxicity of copper sulfate (VI) (CuSO4) by measuring the expression of genes encoding some key enzymes involved in protection against oxidative stress: glutathione peroxidase (gpx), cytochrome P450 (cyp1a), heat shock protein (hsp70) and superoxide dismutase (sod). Hatchlings of rainbow trout (Oncorhynchus mykiss) were used as biological material. The test individuals were exposed for 2 hours to CuO-NPs and CuSO4 in the concentration range of 4–256 mg dm−3. Individuals exposed to all concentrations of CuO-NPs showed an increase in the expression of gpx, cyp1a and hsp70 genes, and a decrease in sod gene expression, while the hatchlings exposed to CuSO4 exhibited an increase in the expression of all genes. The expression of hsp70 and sod genes in the hatchlings exposed to CuSO4 was higher than in the CuO-NP treatment, while the expression levels of gpx and cyp1a were similar. Based on the obtained results, potentially higher toxicity of CuSO4 was observed in the hatchlings of Oncorhynchus mykiss. However, the elevated expression of gpx, cyp1a and hsp70 after the exposure to CuO-NPs, indicates toxicity via oxidative stress. The decrease (sod in hatchlings exposed to CuO-NPs) or increase of gene expression was not dose-dependent. The presented study confirms a need for further research regarding the toxicity of metal nanoparticles to aquatic organisms and the necessity for monitoring the release of CuO-NPs into the aquatic environment.https://www.tandfonline.com/doi/10.1080/24750263.2024.2385820Oncorhynchus mykisscopper oxide nanoparticlescopper (II) sulfateoxidative stressaquatic toxicologyecotoxicology |
| spellingShingle | A. Sielska M. Kowalska-Góralska I. Szućko-Kociuba L. Skuza Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) The European Zoological Journal Oncorhynchus mykiss copper oxide nanoparticles copper (II) sulfate oxidative stress aquatic toxicology ecotoxicology |
| title | Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) |
| title_full | Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) |
| title_fullStr | Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) |
| title_full_unstemmed | Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) |
| title_short | Comparison of the effects of copper oxide nanoparticles (CuO-NPs) and copper (II) sulfate on oxidative stress parameters in rainbow trout hatchlings (Oncorhynchus mykiss) |
| title_sort | comparison of the effects of copper oxide nanoparticles cuo nps and copper ii sulfate on oxidative stress parameters in rainbow trout hatchlings oncorhynchus mykiss |
| topic | Oncorhynchus mykiss copper oxide nanoparticles copper (II) sulfate oxidative stress aquatic toxicology ecotoxicology |
| url | https://www.tandfonline.com/doi/10.1080/24750263.2024.2385820 |
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