In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination
Recently, the use of a cancer deoxyribonucleic acid (DNA) vaccine encoding tumor-associated antigens has emerged as an immunotherapeutic strategy. In this study, we monitored tumor growth inhibition by pcDNA3-hMUC1 immunization in mice using optical imaging. To determine the anti-hMUC1-associated im...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2007-09-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2007.00027 |
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| author | Yong Hyun Jeon Yun Choi Hyun Joo Kim Joo Hyun Kang Chul Woo Kim Jae Min Jeong Dong Soo Lee June-Key Chung |
| author_facet | Yong Hyun Jeon Yun Choi Hyun Joo Kim Joo Hyun Kang Chul Woo Kim Jae Min Jeong Dong Soo Lee June-Key Chung |
| author_sort | Yong Hyun Jeon |
| collection | DOAJ |
| description | Recently, the use of a cancer deoxyribonucleic acid (DNA) vaccine encoding tumor-associated antigens has emerged as an immunotherapeutic strategy. In this study, we monitored tumor growth inhibition by pcDNA3-hMUC1 immunization in mice using optical imaging. To determine the anti-hMUC1-associated immune response generated by pcDNA3.1 or pcDNA3-hMUC1, we determined the concentration of interferon-γ (IFN-γ) protein and CD8+IFN-γ cell numbers among lymphocytes from the draining lymph nodes of mice immunized with pcDNA3.1 or pcDNA3-hMUC1. After subcutaneously injecting CT26/hMUC1-F luc into mice immunized with pcDNA3-hMUC1, we monitored in vivo tumor growth inhibition using an optical imaging method. The concentration of IFN-γ protein in pcDNA3-hMUC1 was higher than that of the pcDNA3.1 group (2.7 ⩽ 0.08 ng/mL and 1.6 ± 0.07 ng/mL, respectively, p < .001. The number of hMUC1-associated CD8+IFN-γ cells in pcDNA3-hMUC1-immunized animals was 30-fold higher than in the pcDNA3.1 group. Bioluminescent images showed tumor growth inhibition in pcDNA3-hMUC1 immunized animals up to 25 days after immunization. A good correlation ( r 2 = .9076: pcDNA3/hMUC1 group; r 2 = .7428: pcDNA3.1 group) was observed between bioluminescence signals and tumor weights in two mice in each group. We conclude that optical bioluminescent imaging offers a useful means of monitoring the antitumor effects of cancer DNA immunization in living animals. |
| format | Article |
| id | doaj-art-4885a423606f4e1a8338a2113fcedb86 |
| institution | DOAJ |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-4885a423606f4e1a8338a2113fcedb862025-08-20T02:43:12ZengSAGE PublishingMolecular Imaging1536-01212007-09-01610.2310/7290.2007.0002710.2310_7290.2007.00027In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 VaccinationYong Hyun JeonYun ChoiHyun Joo KimJoo Hyun KangChul Woo KimJae Min JeongDong Soo LeeJune-Key ChungRecently, the use of a cancer deoxyribonucleic acid (DNA) vaccine encoding tumor-associated antigens has emerged as an immunotherapeutic strategy. In this study, we monitored tumor growth inhibition by pcDNA3-hMUC1 immunization in mice using optical imaging. To determine the anti-hMUC1-associated immune response generated by pcDNA3.1 or pcDNA3-hMUC1, we determined the concentration of interferon-γ (IFN-γ) protein and CD8+IFN-γ cell numbers among lymphocytes from the draining lymph nodes of mice immunized with pcDNA3.1 or pcDNA3-hMUC1. After subcutaneously injecting CT26/hMUC1-F luc into mice immunized with pcDNA3-hMUC1, we monitored in vivo tumor growth inhibition using an optical imaging method. The concentration of IFN-γ protein in pcDNA3-hMUC1 was higher than that of the pcDNA3.1 group (2.7 ⩽ 0.08 ng/mL and 1.6 ± 0.07 ng/mL, respectively, p < .001. The number of hMUC1-associated CD8+IFN-γ cells in pcDNA3-hMUC1-immunized animals was 30-fold higher than in the pcDNA3.1 group. Bioluminescent images showed tumor growth inhibition in pcDNA3-hMUC1 immunized animals up to 25 days after immunization. A good correlation ( r 2 = .9076: pcDNA3/hMUC1 group; r 2 = .7428: pcDNA3.1 group) was observed between bioluminescence signals and tumor weights in two mice in each group. We conclude that optical bioluminescent imaging offers a useful means of monitoring the antitumor effects of cancer DNA immunization in living animals.https://doi.org/10.2310/7290.2007.00027 |
| spellingShingle | Yong Hyun Jeon Yun Choi Hyun Joo Kim Joo Hyun Kang Chul Woo Kim Jae Min Jeong Dong Soo Lee June-Key Chung In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination Molecular Imaging |
| title | In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination |
| title_full | In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination |
| title_fullStr | In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination |
| title_full_unstemmed | In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination |
| title_short | In Vivo Bioluminescence Visualization of Antitumor Effects by Human MUC1 Vaccination |
| title_sort | in vivo bioluminescence visualization of antitumor effects by human muc1 vaccination |
| url | https://doi.org/10.2310/7290.2007.00027 |
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