Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia
Biliary intraepithelial neoplasia (BilIN) is a precursor lesion of hilar/perihilar and extrahepatic cholangiocarcinoma. BilIN represents the process of multistep cholangiocarcinogenesis and is the biliary counterpart of pancreatic intraepithelial neoplasia (PanIN). This study was performed to clarif...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2014/678260 |
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| author | Yasunori Sato Kenichi Harada Motoko Sasaki Yasuni Nakanuma |
| author_facet | Yasunori Sato Kenichi Harada Motoko Sasaki Yasuni Nakanuma |
| author_sort | Yasunori Sato |
| collection | DOAJ |
| description | Biliary intraepithelial neoplasia (BilIN) is a precursor lesion of hilar/perihilar and extrahepatic cholangiocarcinoma. BilIN represents the process of multistep cholangiocarcinogenesis and is the biliary counterpart of pancreatic intraepithelial neoplasia (PanIN). This study was performed to clarify the histological characteristics of BilIN in relation to PanIN. Using paraffin-embedded tissue sections of surgically resected specimens of cholangiocarcinoma associated with BilIN and pancreatic ductal adenocarcinoma associated with PanIN, immunohistochemical staining was performed using primary antibodies against MUC1, MUC2, MUC5AC, cyclin D1, p21, p53, and S100P. For mucin staining, Alcian blue pH 2.5 was used. Most of the molecules examined here showed similar expression patterns in BilIN and PanIN, in which their expression tended to increase along with the increase in atypia of the epithelial lesions. Significant differences were observed in the increase in mucin production and the expression of S100P in PanIN-1 and the expression of p53 in PanIN-3, when compared with those in BilIN of a corresponding grade. These results suggest that cholangiocarcinoma and pancreatic ductal adenocarcinoma share, at least in part, a common carcinogenic process and further confirm that BilIN can be regarded as the biliary counterpart of PanIN. |
| format | Article |
| id | doaj-art-4877ed6af009488c8dbf7a2075c199b9 |
| institution | Kabale University |
| issn | 2090-3448 2090-3456 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hepatology |
| spelling | doaj-art-4877ed6af009488c8dbf7a2075c199b92025-02-03T06:07:45ZengWileyInternational Journal of Hepatology2090-34482090-34562014-01-01201410.1155/2014/678260678260Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial NeoplasiaYasunori Sato0Kenichi Harada1Motoko Sasaki2Yasuni Nakanuma3Department of Human Pathology, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa 920-8640, JapanDepartment of Human Pathology, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa 920-8640, JapanDepartment of Human Pathology, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa 920-8640, JapanDepartment of Human Pathology, Kanazawa University Graduate School of Medicine, 13-1 Takara-machi, Kanazawa 920-8640, JapanBiliary intraepithelial neoplasia (BilIN) is a precursor lesion of hilar/perihilar and extrahepatic cholangiocarcinoma. BilIN represents the process of multistep cholangiocarcinogenesis and is the biliary counterpart of pancreatic intraepithelial neoplasia (PanIN). This study was performed to clarify the histological characteristics of BilIN in relation to PanIN. Using paraffin-embedded tissue sections of surgically resected specimens of cholangiocarcinoma associated with BilIN and pancreatic ductal adenocarcinoma associated with PanIN, immunohistochemical staining was performed using primary antibodies against MUC1, MUC2, MUC5AC, cyclin D1, p21, p53, and S100P. For mucin staining, Alcian blue pH 2.5 was used. Most of the molecules examined here showed similar expression patterns in BilIN and PanIN, in which their expression tended to increase along with the increase in atypia of the epithelial lesions. Significant differences were observed in the increase in mucin production and the expression of S100P in PanIN-1 and the expression of p53 in PanIN-3, when compared with those in BilIN of a corresponding grade. These results suggest that cholangiocarcinoma and pancreatic ductal adenocarcinoma share, at least in part, a common carcinogenic process and further confirm that BilIN can be regarded as the biliary counterpart of PanIN.http://dx.doi.org/10.1155/2014/678260 |
| spellingShingle | Yasunori Sato Kenichi Harada Motoko Sasaki Yasuni Nakanuma Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia International Journal of Hepatology |
| title | Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia |
| title_full | Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia |
| title_fullStr | Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia |
| title_full_unstemmed | Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia |
| title_short | Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia |
| title_sort | histological characterization of biliary intraepithelial neoplasia with respect to pancreatic intraepithelial neoplasia |
| url | http://dx.doi.org/10.1155/2014/678260 |
| work_keys_str_mv | AT yasunorisato histologicalcharacterizationofbiliaryintraepithelialneoplasiawithrespecttopancreaticintraepithelialneoplasia AT kenichiharada histologicalcharacterizationofbiliaryintraepithelialneoplasiawithrespecttopancreaticintraepithelialneoplasia AT motokosasaki histologicalcharacterizationofbiliaryintraepithelialneoplasiawithrespecttopancreaticintraepithelialneoplasia AT yasuninakanuma histologicalcharacterizationofbiliaryintraepithelialneoplasiawithrespecttopancreaticintraepithelialneoplasia |