A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat
Abstract Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-03-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57936-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849774520436523008 |
|---|---|
| author | Xia Yang Leilei Zhang Jiajie Wei Lexin Liu Di Liu Xiangning Yan Minjie Yuan Lingran Zhang Ning Zhang Yan Ren Feng Chen |
| author_facet | Xia Yang Leilei Zhang Jiajie Wei Lexin Liu Di Liu Xiangning Yan Minjie Yuan Lingran Zhang Ning Zhang Yan Ren Feng Chen |
| author_sort | Xia Yang |
| collection | DOAJ |
| description | Abstract Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of TaCAT2 regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2Ser214, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2Ser214 than in TaCAT2Thr214, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2Ser214 wheat plants. Furthermore, the function of TaSnRK1α regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants. |
| format | Article |
| id | doaj-art-487274dd0dd04aaf9fcf784cdf322c0b |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-487274dd0dd04aaf9fcf784cdf322c0b2025-08-20T03:01:41ZengNature PortfolioNature Communications2041-17232025-03-0116111510.1038/s41467-025-57936-xA TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheatXia Yang0Leilei Zhang1Jiajie Wei2Lexin Liu3Di Liu4Xiangning Yan5Minjie Yuan6Lingran Zhang7Ning Zhang8Yan Ren9Feng Chen10Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityKey Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping /Agronomy College, Henan Agricultural UniversityAbstract Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of TaCAT2 regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2Ser214, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2Ser214 than in TaCAT2Thr214, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2Ser214 wheat plants. Furthermore, the function of TaSnRK1α regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants.https://doi.org/10.1038/s41467-025-57936-x |
| spellingShingle | Xia Yang Leilei Zhang Jiajie Wei Lexin Liu Di Liu Xiangning Yan Minjie Yuan Lingran Zhang Ning Zhang Yan Ren Feng Chen A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat Nature Communications |
| title | A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat |
| title_full | A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat |
| title_fullStr | A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat |
| title_full_unstemmed | A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat |
| title_short | A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat |
| title_sort | tasnrk1α tacat2 model mediates resistance to fusarium crown rot by scavenging ros in common wheat |
| url | https://doi.org/10.1038/s41467-025-57936-x |
| work_keys_str_mv | AT xiayang atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT leileizhang atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT jiajiewei atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT lexinliu atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT diliu atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT xiangningyan atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT minjieyuan atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT lingranzhang atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT ningzhang atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT yanren atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT fengchen atasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT xiayang tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT leileizhang tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT jiajiewei tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT lexinliu tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT diliu tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT xiangningyan tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT minjieyuan tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT lingranzhang tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT ningzhang tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT yanren tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat AT fengchen tasnrk1atacat2modelmediatesresistancetofusariumcrownrotbyscavengingrosincommonwheat |