Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis
Ceftiofur nanoemulsion (CNE) with stable property was made of Tween-80, glycerine, soybean oil, water, ceftiofur and dimethyl sulfoxide (DMSO), which was detected as an oil-in-water nanoemulsion with an average diameter of 60.54 nm. To investigate the distribution of CNE in milk, ceftiofur sodium (C...
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Zhejiang University Press
2018-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.171 |
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| author | LIANG Siyuan WANG Yuemin HU Songhua |
| author_facet | LIANG Siyuan WANG Yuemin HU Songhua |
| author_sort | LIANG Siyuan |
| collection | DOAJ |
| description | Ceftiofur nanoemulsion (CNE) with stable property was made of Tween-80, glycerine, soybean oil, water, ceftiofur and dimethyl sulfoxide (DMSO), which was detected as an oil-in-water nanoemulsion with an average diameter of 60.54 nm. To investigate the distribution of CNE in milk, ceftiofur sodium (CS) and CNE were incubated for 12 h at 37℃ in milk containing Staphylococcus aureus or Escherichia coli, and then the colony was counted in upper, middle and lower parts of milk. The results showed that from upper part to lower part, the colony counts were increased successively in the CNE-treated milk, which was on the contrary in the CS-treated milk, indicating that CNE tended to distribute in the upper part, while CS tended to distribute in the lower part. To further explore the therapeutic effect of CNE on bacterial infection, mice infected S. aureus were injected with CS or CNE at a dose of 19.1, 38.2 and 57.3 μmol/kg, respectively. In the CS-treated group, the survival rates of mice were 40%, 50% and 70%, respectively; while in the CNE-treated group, the survival rates of mice were 40%, 70%and 80%, respectively. Therefore, CNE had superior therapeutic effect. This study provides reference values for clinical use of CNE to treat bacterial infection. |
| format | Article |
| id | doaj-art-4868e86e95e142e3b2bf2113d5ecaddc |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-4868e86e95e142e3b2bf2113d5ecaddc2025-08-20T02:47:32ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552018-03-014424725210.3785/j.issn.1008-9209.2018.04.17110089209Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitisLIANG SiyuanWANG YueminHU SonghuaCeftiofur nanoemulsion (CNE) with stable property was made of Tween-80, glycerine, soybean oil, water, ceftiofur and dimethyl sulfoxide (DMSO), which was detected as an oil-in-water nanoemulsion with an average diameter of 60.54 nm. To investigate the distribution of CNE in milk, ceftiofur sodium (CS) and CNE were incubated for 12 h at 37℃ in milk containing Staphylococcus aureus or Escherichia coli, and then the colony was counted in upper, middle and lower parts of milk. The results showed that from upper part to lower part, the colony counts were increased successively in the CNE-treated milk, which was on the contrary in the CS-treated milk, indicating that CNE tended to distribute in the upper part, while CS tended to distribute in the lower part. To further explore the therapeutic effect of CNE on bacterial infection, mice infected S. aureus were injected with CS or CNE at a dose of 19.1, 38.2 and 57.3 μmol/kg, respectively. In the CS-treated group, the survival rates of mice were 40%, 50% and 70%, respectively; while in the CNE-treated group, the survival rates of mice were 40%, 70%and 80%, respectively. Therefore, CNE had superior therapeutic effect. This study provides reference values for clinical use of CNE to treat bacterial infection.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.171ceftiofur nanoemulsionbovine mastitis<italic>Staphylococcus aureus</italic><italic>Escherichia coli</italic> |
| spellingShingle | LIANG Siyuan WANG Yuemin HU Songhua Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis 浙江大学学报. 农业与生命科学版 ceftiofur nanoemulsion bovine mastitis <italic>Staphylococcus aureus</italic> <italic>Escherichia coli</italic> |
| title | Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| title_full | Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| title_fullStr | Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| title_full_unstemmed | Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| title_short | Preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| title_sort | preparation of ceftiofur nanoemulsion and its antimicrobial effect on major pathogenic bacteria of bovine mastitis |
| topic | ceftiofur nanoemulsion bovine mastitis <italic>Staphylococcus aureus</italic> <italic>Escherichia coli</italic> |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.04.171 |
| work_keys_str_mv | AT liangsiyuan preparationofceftiofurnanoemulsionanditsantimicrobialeffectonmajorpathogenicbacteriaofbovinemastitis AT wangyuemin preparationofceftiofurnanoemulsionanditsantimicrobialeffectonmajorpathogenicbacteriaofbovinemastitis AT husonghua preparationofceftiofurnanoemulsionanditsantimicrobialeffectonmajorpathogenicbacteriaofbovinemastitis |