Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion

Background: Pleural effusions are caused by various conditions, including tuberculosis and malignancies. Accurate differentiation between tuberculous pleural effusion (TPE) and non-tuberculous pleural effusion (NTPE) is crucial for effective treatment outcomes. While adenosine deaminase (ADA) levels...

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Main Authors: R. Sai Deeksha, Basava, Shraddha Acharya
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-01-01
Series:International Journal of Medicine and Health Development
Subjects:
Online Access:https://doi.org/10.4103/ijmh.ijmh_59_24
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author R. Sai Deeksha
Basava
Shraddha Acharya
author_facet R. Sai Deeksha
Basava
Shraddha Acharya
author_sort R. Sai Deeksha
collection DOAJ
description Background: Pleural effusions are caused by various conditions, including tuberculosis and malignancies. Accurate differentiation between tuberculous pleural effusion (TPE) and non-tuberculous pleural effusion (NTPE) is crucial for effective treatment outcomes. While adenosine deaminase (ADA) levels are commonly used in diagnosing TPE, the pleural fluid LDH-to-ADA ratio is a potentially more accurate diagnostic marker. Objective: To evaluate the effectiveness of the LDH-to-ADA ratio in distinguishing between TPE and NTPE, especially in cases of increased ADA levels. Materials and Methods: This retrospective study included 80 patients who underwent their first thoracentesis with a pleural fluid ADA level of 40 IU/L or higher. Patients were categorized into TPE (n = 40) and NTPE (n = 40) groups based on clinical, radiological, and laboratory findings. Pleural fluid parameters, including LDH and ADA levels, were analyzed. Receiver operating characteristic (ROC) curve analysis was conducted to determine the diagnostic accuracy of the LDH-to-ADA ratio, with cut-off values assessed for the sensitivity and specificity. Odds ratios were calculated to evaluate the association between the LDH-to-ADA ratio and pleural effusion types. Results: ROC analysis demonstrated that the pleural fluid LDH-to-ADA ratio in comparison to the ADA levels was superior in identifying TPE, with an AUC of 0.86 (sensitivity: 75%; specificity: 90.1%), and for NTPE, with an AUC of 0.90 (sensitivity: 80%; specificity: 77.9%). The LDH-to-ADA ratio provided a strong correlation with TPE and NTPE, confirmed by both crude and adjusted odds ratios, respectively. Conclusion: The pleural fluid LDH-to-ADA ratio is a highly effective diagnostic tool for distinguishing between TPE and NTPE. It offers superior diagnostic performance compared to ADA levels alone, with well-defined cut off values enhancing its utility in clinical practice for accurate diagnosis and management of pleural effusions.
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spelling doaj-art-4866f35e77e047e9b814860e254e54fe2025-08-20T02:56:27ZengWolters Kluwer Medknow PublicationsInternational Journal of Medicine and Health Development2635-36952667-28632025-01-01301757910.4103/ijmh.ijmh_59_24Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular EffusionR. Sai DeekshaBasavaShraddha AcharyaBackground: Pleural effusions are caused by various conditions, including tuberculosis and malignancies. Accurate differentiation between tuberculous pleural effusion (TPE) and non-tuberculous pleural effusion (NTPE) is crucial for effective treatment outcomes. While adenosine deaminase (ADA) levels are commonly used in diagnosing TPE, the pleural fluid LDH-to-ADA ratio is a potentially more accurate diagnostic marker. Objective: To evaluate the effectiveness of the LDH-to-ADA ratio in distinguishing between TPE and NTPE, especially in cases of increased ADA levels. Materials and Methods: This retrospective study included 80 patients who underwent their first thoracentesis with a pleural fluid ADA level of 40 IU/L or higher. Patients were categorized into TPE (n = 40) and NTPE (n = 40) groups based on clinical, radiological, and laboratory findings. Pleural fluid parameters, including LDH and ADA levels, were analyzed. Receiver operating characteristic (ROC) curve analysis was conducted to determine the diagnostic accuracy of the LDH-to-ADA ratio, with cut-off values assessed for the sensitivity and specificity. Odds ratios were calculated to evaluate the association between the LDH-to-ADA ratio and pleural effusion types. Results: ROC analysis demonstrated that the pleural fluid LDH-to-ADA ratio in comparison to the ADA levels was superior in identifying TPE, with an AUC of 0.86 (sensitivity: 75%; specificity: 90.1%), and for NTPE, with an AUC of 0.90 (sensitivity: 80%; specificity: 77.9%). The LDH-to-ADA ratio provided a strong correlation with TPE and NTPE, confirmed by both crude and adjusted odds ratios, respectively. Conclusion: The pleural fluid LDH-to-ADA ratio is a highly effective diagnostic tool for distinguishing between TPE and NTPE. It offers superior diagnostic performance compared to ADA levels alone, with well-defined cut off values enhancing its utility in clinical practice for accurate diagnosis and management of pleural effusions.https://doi.org/10.4103/ijmh.ijmh_59_24adaldhtuberculosis
spellingShingle R. Sai Deeksha
Basava
Shraddha Acharya
Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
International Journal of Medicine and Health Development
ada
ldh
tuberculosis
title Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
title_full Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
title_fullStr Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
title_full_unstemmed Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
title_short Comparison of Pleural Fluid Adenosine Deaminase with the Lactate Dehydrogenase/Adenosine Deaminase Ratio in Differentiating Tubercular Pleural Effusion from Non-tubercular Effusion
title_sort comparison of pleural fluid adenosine deaminase with the lactate dehydrogenase adenosine deaminase ratio in differentiating tubercular pleural effusion from non tubercular effusion
topic ada
ldh
tuberculosis
url https://doi.org/10.4103/ijmh.ijmh_59_24
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