In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci
Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like pr...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/8578936 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561213398056960 |
|---|---|
| author | Diana G. Rios-Valencia Edgar O. López-Villegas Dylan Diaz Chiguer Adrian Marquez Navarro Ruben D. Díaz-Martín Benjamin Nogueda-Torres Javier R. Ambrosio |
| author_facet | Diana G. Rios-Valencia Edgar O. López-Villegas Dylan Diaz Chiguer Adrian Marquez Navarro Ruben D. Díaz-Martín Benjamin Nogueda-Torres Javier R. Ambrosio |
| author_sort | Diana G. Rios-Valencia |
| collection | DOAJ |
| description | Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like protein in cysticerci of Taenia crassiceps and Taenia solium using the deduced amino acid sequence of T. solium septin 4 (SEPT4_Tsm), to design and synthesize a derived immunogenic peptide (residues 88 to 103), to prepare a specific rabbit polyclonal antibody, and to examine the effects of FCF at different concentrations and exposure times on an in vitro culture of T. crassiceps cysticerci. In vitro, FCF altered the morphology and motility of T. crassiceps cysticerci, and its effects were reversible under specific concentrations. In addition, we observed by ultrastructural observation that FCF alters the cellular subunit of the protonephridial system of cestodes, where disruption of the axoneme pattern of flame cells was observed. The rabbit polyclonal antibody prepared against the synthetic peptide recognized a major band of 41 kDa in both parasites. Our results establish the importance of SEPT4_Tsm in the dynamics and survival of taeniid cysticerci, as well as their susceptibility to FCF. This is also the first report that a septin is present in the cytoskeleton of taeniids. |
| format | Article |
| id | doaj-art-4865737ec82e4940ad4f01f603b00dfd |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-4865737ec82e4940ad4f01f603b00dfd2025-02-03T01:25:38ZengWileyJournal of Parasitology Research2090-00232090-00312019-01-01201910.1155/2019/85789368578936In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid CysticerciDiana G. Rios-Valencia0Edgar O. López-Villegas1Dylan Diaz Chiguer2Adrian Marquez Navarro3Ruben D. Díaz-Martín4Benjamin Nogueda-Torres5Javier R. Ambrosio6Departamento de Parasitología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, 11340 Ciudad de México, MexicoCentral de Instrumentación de Microscopia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Ciudad de México, MexicoClínica de Especialidades Indianilla, Dirección Médica, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, 06720 Ciudad de México, MexicoComisión Federal para la Protección contra Riesgos Sanitarios, 03810 Ciudad de México, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Ciudad de México, MexicoDepartamento de Parasitología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, 11340 Ciudad de México, MexicoDepartamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 Ciudad de México, MexicoCytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. The aim of this work was to determine the presence of a septin-like protein in cysticerci of Taenia crassiceps and Taenia solium using the deduced amino acid sequence of T. solium septin 4 (SEPT4_Tsm), to design and synthesize a derived immunogenic peptide (residues 88 to 103), to prepare a specific rabbit polyclonal antibody, and to examine the effects of FCF at different concentrations and exposure times on an in vitro culture of T. crassiceps cysticerci. In vitro, FCF altered the morphology and motility of T. crassiceps cysticerci, and its effects were reversible under specific concentrations. In addition, we observed by ultrastructural observation that FCF alters the cellular subunit of the protonephridial system of cestodes, where disruption of the axoneme pattern of flame cells was observed. The rabbit polyclonal antibody prepared against the synthetic peptide recognized a major band of 41 kDa in both parasites. Our results establish the importance of SEPT4_Tsm in the dynamics and survival of taeniid cysticerci, as well as their susceptibility to FCF. This is also the first report that a septin is present in the cytoskeleton of taeniids.http://dx.doi.org/10.1155/2019/8578936 |
| spellingShingle | Diana G. Rios-Valencia Edgar O. López-Villegas Dylan Diaz Chiguer Adrian Marquez Navarro Ruben D. Díaz-Martín Benjamin Nogueda-Torres Javier R. Ambrosio In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci Journal of Parasitology Research |
| title | In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci |
| title_full | In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci |
| title_fullStr | In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci |
| title_full_unstemmed | In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci |
| title_short | In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci |
| title_sort | in vitro analyses reveal the effect of synthetic cytokinin forchlorfenuron fcf on a septin like protein of taeniid cysticerci |
| url | http://dx.doi.org/10.1155/2019/8578936 |
| work_keys_str_mv | AT dianagriosvalencia invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT edgarolopezvillegas invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT dylandiazchiguer invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT adrianmarqueznavarro invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT rubenddiazmartin invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT benjaminnoguedatorres invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci AT javierrambrosio invitroanalysesrevealtheeffectofsyntheticcytokininforchlorfenuronfcfonaseptinlikeproteinoftaeniidcysticerci |