Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice

Ulcerative colitis (UC) is effectively alleviated by <i>Saccharomyces boulardii</i> (<i>S. boulardii</i>), an important probiotic. Postbiotics, defined as beneficial non-viable microorganisms and/or their components, can potentially improve gut health. In this study, we utili...

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Main Authors: Yuxin Jin, Xinge Xu, Kunlun Huang, Zhihong Liang
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/7/1109
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author Yuxin Jin
Xinge Xu
Kunlun Huang
Zhihong Liang
author_facet Yuxin Jin
Xinge Xu
Kunlun Huang
Zhihong Liang
author_sort Yuxin Jin
collection DOAJ
description Ulcerative colitis (UC) is effectively alleviated by <i>Saccharomyces boulardii</i> (<i>S. boulardii</i>), an important probiotic. Postbiotics, defined as beneficial non-viable microorganisms and/or their components, can potentially improve gut health. In this study, we utilized <i>S. boulardii</i> to prepare postbiotics via freeze-drying and spray-drying methods, characterized the resulting postbiotics, and investigated their efficacy and underlying mechanisms in preventing UC. In a mouse model of UC induced by dextran sulfate sodium (DSS), we found that prevention with two forms of <i>S. boulardii</i> postbiotics alleviated colitis symptoms triggered by DSS, mitigated colon tissue damage, maintained the distribution of intestinal occludin and ZO-1 proteins, and suppressed the secretion and expression of TNF-α, IL-1β, and IL-6 in serum and colon tissues. Additionally, <i>S. boulardii</i> postbiotics mitigated dysbiosis by modulating gut microbiota composition, including the balance between Bacteroidota and Firmicutes (F/B), as well as the levels of <i>Akkermansia</i>, <i>Muribaculaceae</i>, <i>Dubosiella</i>, and <i>Turicibacter</i>. In conclusion, as a novel biotherapeutic agent, <i>S. boulardii</i> postbiotics effectively prevent DSS-induced UC in mice. Compared to live <i>S. boulardii</i>, postbiotics may hold greater potential for UC prevention.
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spelling doaj-art-486250c02f844792b000ea56f2218f8a2025-08-20T02:15:58ZengMDPI AGFoods2304-81582025-03-01147110910.3390/foods14071109Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in MiceYuxin Jin0Xinge Xu1Kunlun Huang2Zhihong Liang3College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaUlcerative colitis (UC) is effectively alleviated by <i>Saccharomyces boulardii</i> (<i>S. boulardii</i>), an important probiotic. Postbiotics, defined as beneficial non-viable microorganisms and/or their components, can potentially improve gut health. In this study, we utilized <i>S. boulardii</i> to prepare postbiotics via freeze-drying and spray-drying methods, characterized the resulting postbiotics, and investigated their efficacy and underlying mechanisms in preventing UC. In a mouse model of UC induced by dextran sulfate sodium (DSS), we found that prevention with two forms of <i>S. boulardii</i> postbiotics alleviated colitis symptoms triggered by DSS, mitigated colon tissue damage, maintained the distribution of intestinal occludin and ZO-1 proteins, and suppressed the secretion and expression of TNF-α, IL-1β, and IL-6 in serum and colon tissues. Additionally, <i>S. boulardii</i> postbiotics mitigated dysbiosis by modulating gut microbiota composition, including the balance between Bacteroidota and Firmicutes (F/B), as well as the levels of <i>Akkermansia</i>, <i>Muribaculaceae</i>, <i>Dubosiella</i>, and <i>Turicibacter</i>. In conclusion, as a novel biotherapeutic agent, <i>S. boulardii</i> postbiotics effectively prevent DSS-induced UC in mice. Compared to live <i>S. boulardii</i>, postbiotics may hold greater potential for UC prevention.https://www.mdpi.com/2304-8158/14/7/1109<i>Saccharomyces boulardii</i>postbioticsprobioticulcerative colitisgut microbiotainflammatory
spellingShingle Yuxin Jin
Xinge Xu
Kunlun Huang
Zhihong Liang
Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
Foods
<i>Saccharomyces boulardii</i>
postbiotics
probiotic
ulcerative colitis
gut microbiota
inflammatory
title Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
title_full Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
title_fullStr Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
title_full_unstemmed Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
title_short Pre-Administration of <i>Saccharomyces boulardii</i>-Derived Postbiotics Effectively Prevents Dextran Sulfate Sodium-Induced Colitis in Mice
title_sort pre administration of i saccharomyces boulardii i derived postbiotics effectively prevents dextran sulfate sodium induced colitis in mice
topic <i>Saccharomyces boulardii</i>
postbiotics
probiotic
ulcerative colitis
gut microbiota
inflammatory
url https://www.mdpi.com/2304-8158/14/7/1109
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AT kunlunhuang preadministrationofisaccharomycesboulardiiiderivedpostbioticseffectivelypreventsdextransulfatesodiuminducedcolitisinmice
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