Encapsulation for efficient cryopreservation
Cryopreservation is a fundamental technology in biomedical research, regenerative medicine, and tissue engineering, enabling the long-term storage of cells, tissues, and organs. However, its effectiveness is limited by challenges such as intracellular ice formation, cryoprotectant toxicity, and redu...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-08-01
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| Series: | Frigid Zone Medicine |
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| Online Access: | https://doi.org/10.1515/fzm-2025-0008 |
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| author | Memon Kashan Zhang Bing Fareed Muhammad Azam Zhao Gang |
| author_facet | Memon Kashan Zhang Bing Fareed Muhammad Azam Zhao Gang |
| author_sort | Memon Kashan |
| collection | DOAJ |
| description | Cryopreservation is a fundamental technology in biomedical research, regenerative medicine, and tissue engineering, enabling the long-term storage of cells, tissues, and organs. However, its effectiveness is limited by challenges such as intracellular ice formation, cryoprotectant toxicity, and reduced post-thaw viability. This review explores the crucial role of encapsulation in enhancing cryopreservation efficiency, with a focus on recent advances in materials science, bioengineering, and cryobiology. Emerging technologies, such as nanotechnology and stimuli-responsive polymers, are transforming encapsulation strategies. Innovations such as microfluidic systems offer precise control over cooling rates and cryoprotectant distribution, thereby mitigating conventional limitations. The review also addresses current obstacles related to scaling up encapsulation processes and ensuring the long-term biocompatibility and stability of preserved specimens. By synthesizing recent findings, this work provides a comprehensive resource for researchers and clinicians seeking to enhance biopreservation techniques and their applications in contemporary medicine and biotechnology. Finally, the review identifies critical knowledge gaps that must be addressed to improve the efficacy of cryopreservation strategies and advance their clinical translation. |
| format | Article |
| id | doaj-art-485b441cedf34c439e16f6de76ffe435 |
| institution | Kabale University |
| issn | 2719-8073 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Frigid Zone Medicine |
| spelling | doaj-art-485b441cedf34c439e16f6de76ffe4352025-08-25T06:10:32ZengDe GruyterFrigid Zone Medicine2719-80732025-08-0152738010.1515/fzm-2025-0008Encapsulation for efficient cryopreservationMemon Kashan0Zhang Bing1Fareed Muhammad Azam2Zhao Gang3Department of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei230026, ChinaDepartment of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei230026, ChinaDepartment of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei230026, ChinaDepartment of Electronic Engineering and Information Science, University of Science and Technology of China, Hefei230026, ChinaCryopreservation is a fundamental technology in biomedical research, regenerative medicine, and tissue engineering, enabling the long-term storage of cells, tissues, and organs. However, its effectiveness is limited by challenges such as intracellular ice formation, cryoprotectant toxicity, and reduced post-thaw viability. This review explores the crucial role of encapsulation in enhancing cryopreservation efficiency, with a focus on recent advances in materials science, bioengineering, and cryobiology. Emerging technologies, such as nanotechnology and stimuli-responsive polymers, are transforming encapsulation strategies. Innovations such as microfluidic systems offer precise control over cooling rates and cryoprotectant distribution, thereby mitigating conventional limitations. The review also addresses current obstacles related to scaling up encapsulation processes and ensuring the long-term biocompatibility and stability of preserved specimens. By synthesizing recent findings, this work provides a comprehensive resource for researchers and clinicians seeking to enhance biopreservation techniques and their applications in contemporary medicine and biotechnology. Finally, the review identifies critical knowledge gaps that must be addressed to improve the efficacy of cryopreservation strategies and advance their clinical translation.https://doi.org/10.1515/fzm-2025-0008cryopreservationencapsulationhydrogelsbiomaterialstissue engineeringregenerative medicinenanotechnologysmart polymers |
| spellingShingle | Memon Kashan Zhang Bing Fareed Muhammad Azam Zhao Gang Encapsulation for efficient cryopreservation Frigid Zone Medicine cryopreservation encapsulation hydrogels biomaterials tissue engineering regenerative medicine nanotechnology smart polymers |
| title | Encapsulation for efficient cryopreservation |
| title_full | Encapsulation for efficient cryopreservation |
| title_fullStr | Encapsulation for efficient cryopreservation |
| title_full_unstemmed | Encapsulation for efficient cryopreservation |
| title_short | Encapsulation for efficient cryopreservation |
| title_sort | encapsulation for efficient cryopreservation |
| topic | cryopreservation encapsulation hydrogels biomaterials tissue engineering regenerative medicine nanotechnology smart polymers |
| url | https://doi.org/10.1515/fzm-2025-0008 |
| work_keys_str_mv | AT memonkashan encapsulationforefficientcryopreservation AT zhangbing encapsulationforefficientcryopreservation AT fareedmuhammadazam encapsulationforefficientcryopreservation AT zhaogang encapsulationforefficientcryopreservation |