Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer

Abstract Colorectal cancer (CRC) is a leading global health issue, ranking third in incidence and second in cancer mortality. Immunotherapy, effective mainly in mismatch repair-deficient CRC, may benefit from dietary interventions. This study investigates caffeine’s potential to boost programmed dea...

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Main Authors: Yuechen Liu, Zhengyu Liu, Yating Hu, Yunyan Ling, Shunjie Qing, Yang Liu, Yizhi Zhan, Zhiyong Shen, Yuan Fang, Haijun Deng
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60958-0
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author Yuechen Liu
Zhengyu Liu
Yating Hu
Yunyan Ling
Shunjie Qing
Yang Liu
Yizhi Zhan
Zhiyong Shen
Yuan Fang
Haijun Deng
author_facet Yuechen Liu
Zhengyu Liu
Yating Hu
Yunyan Ling
Shunjie Qing
Yang Liu
Yizhi Zhan
Zhiyong Shen
Yuan Fang
Haijun Deng
author_sort Yuechen Liu
collection DOAJ
description Abstract Colorectal cancer (CRC) is a leading global health issue, ranking third in incidence and second in cancer mortality. Immunotherapy, effective mainly in mismatch repair-deficient CRC, may benefit from dietary interventions. This study investigates caffeine’s potential to boost programmed death-1 (PD-1) immunotherapy efficacy in CRC, revealing that caffeine significantly reduces tumor growth, extends survival, and enhances CD8+ T cell activity in CRC by suppressing kynurenine pathway. Mechanistically, caffeine decreases kynurenine via the Krüppel-like factor 4 (KLF4)- Collagen type XII alpha 1 (COL12A1)- Mitogen-Activated Protein Kinase (MAPK)-Indoleamine 2,3-dioxygenase 1 (IDO1) axis, mitigating CD8+ T cell exhaustion. Combining caffeine with PD-1 therapy further prolongs survival, highlighting the value of integrating nutritional strategies into cancer treatment to improve outcomes and broaden therapeutic options. Here, we show caffeine can enhance PD-1 immunotherapy in CRC by suppressing kynurenine pathway, suggesting its potential as an adjunctive dietary therapy.
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id doaj-art-484170ac28d24c128fa7ab5e62a9e46a
institution Kabale University
issn 2041-1723
language English
publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-484170ac28d24c128fa7ab5e62a9e46a2025-08-20T03:37:38ZengNature PortfolioNature Communications2041-17232025-07-0116111810.1038/s41467-025-60958-0Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancerYuechen Liu0Zhengyu Liu1Yating Hu2Yunyan Ling3Shunjie Qing4Yang Liu5Yizhi Zhan6Zhiyong Shen7Yuan Fang8Haijun Deng9Department of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical UniversityDepartment of Pathology, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of Radiation Oncology, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Nanfang Hospital, Southern Medical UniversityAbstract Colorectal cancer (CRC) is a leading global health issue, ranking third in incidence and second in cancer mortality. Immunotherapy, effective mainly in mismatch repair-deficient CRC, may benefit from dietary interventions. This study investigates caffeine’s potential to boost programmed death-1 (PD-1) immunotherapy efficacy in CRC, revealing that caffeine significantly reduces tumor growth, extends survival, and enhances CD8+ T cell activity in CRC by suppressing kynurenine pathway. Mechanistically, caffeine decreases kynurenine via the Krüppel-like factor 4 (KLF4)- Collagen type XII alpha 1 (COL12A1)- Mitogen-Activated Protein Kinase (MAPK)-Indoleamine 2,3-dioxygenase 1 (IDO1) axis, mitigating CD8+ T cell exhaustion. Combining caffeine with PD-1 therapy further prolongs survival, highlighting the value of integrating nutritional strategies into cancer treatment to improve outcomes and broaden therapeutic options. Here, we show caffeine can enhance PD-1 immunotherapy in CRC by suppressing kynurenine pathway, suggesting its potential as an adjunctive dietary therapy.https://doi.org/10.1038/s41467-025-60958-0
spellingShingle Yuechen Liu
Zhengyu Liu
Yating Hu
Yunyan Ling
Shunjie Qing
Yang Liu
Yizhi Zhan
Zhiyong Shen
Yuan Fang
Haijun Deng
Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
Nature Communications
title Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
title_full Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
title_fullStr Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
title_full_unstemmed Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
title_short Caffeine enhances antitumor T-cell activity by suppressing kynurenine pathway in colorectal cancer
title_sort caffeine enhances antitumor t cell activity by suppressing kynurenine pathway in colorectal cancer
url https://doi.org/10.1038/s41467-025-60958-0
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