Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing
In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gol...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/1432781 |
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| author | Wanida Tangkawsakul Toemsak Srikhirin Kazunari Shinbo Keizo Kato Futao Kaneko Akira Baba |
| author_facet | Wanida Tangkawsakul Toemsak Srikhirin Kazunari Shinbo Keizo Kato Futao Kaneko Akira Baba |
| author_sort | Wanida Tangkawsakul |
| collection | DOAJ |
| description | In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test. We obtained the lowest detection limits of 1.58 × 105 cells/ml for RBC-A and 3.83 × 105 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 108 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing. |
| format | Article |
| id | doaj-art-4840a2a553344ea7bdbb8f96af24fa37 |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-4840a2a553344ea7bdbb8f96af24fa372025-08-20T02:38:02ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/14327811432781Application of Long-Range Surface Plasmon Resonance for ABO Blood TypingWanida Tangkawsakul0Toemsak Srikhirin1Kazunari Shinbo2Keizo Kato3Futao Kaneko4Akira Baba5Graduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, 8050 Ikarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanMaterials Science and Engineering Programme, Multidisciplinary Unit and Center of Intelligence Materials and Systems, NANOTEC Center of Excellence at Mahidol University, Department of Physics, Faculty of Science, Mahidol University, Rama 6 Rd., Phayathai, Rajathavee, Bangkok 10400, ThailandGraduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, 8050 Ikarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanGraduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, 8050 Ikarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanGraduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, 8050 Ikarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanGraduate School of Science and Technology and Center for Transdisciplinary Research, Niigata University, 8050 Ikarashi 2-Nocho, Nishi-ku, Niigata 950-2181, JapanIn this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test. We obtained the lowest detection limits of 1.58 × 105 cells/ml for RBC-A and 3.83 × 105 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 108 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing.http://dx.doi.org/10.1155/2016/1432781 |
| spellingShingle | Wanida Tangkawsakul Toemsak Srikhirin Kazunari Shinbo Keizo Kato Futao Kaneko Akira Baba Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing International Journal of Analytical Chemistry |
| title | Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing |
| title_full | Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing |
| title_fullStr | Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing |
| title_full_unstemmed | Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing |
| title_short | Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing |
| title_sort | application of long range surface plasmon resonance for abo blood typing |
| url | http://dx.doi.org/10.1155/2016/1432781 |
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