Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda

Abstract Background Systematic tuberculosis (TB) screening is recommended for all people with HIV (PWH) because of its potential to improve TB outcomes through earlier diagnosis and treatment initiation. As such, systematic screening may be particularly important for men, who experience excess TB pr...

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Main Authors: Lelia H. Chaisson, Fred C. Semitala, Sandra Mwebe, Eileen P. Scully, Jane Katende, Lucy Asege, Martha Nakaye, Alfred O. Andama, Adithya Cattamanchi, Christina Yoon
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Infectious Diseases
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Online Access:https://doi.org/10.1186/s12879-025-10835-4
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author Lelia H. Chaisson
Fred C. Semitala
Sandra Mwebe
Eileen P. Scully
Jane Katende
Lucy Asege
Martha Nakaye
Alfred O. Andama
Adithya Cattamanchi
Christina Yoon
author_facet Lelia H. Chaisson
Fred C. Semitala
Sandra Mwebe
Eileen P. Scully
Jane Katende
Lucy Asege
Martha Nakaye
Alfred O. Andama
Adithya Cattamanchi
Christina Yoon
author_sort Lelia H. Chaisson
collection DOAJ
description Abstract Background Systematic tuberculosis (TB) screening is recommended for all people with HIV (PWH) because of its potential to improve TB outcomes through earlier diagnosis and treatment initiation. As such, systematic screening may be particularly important for men, who experience excess TB prevalence and mortality compared to women. We assessed sex differences among PWH undergoing systematic TB screening, including TB prevalence and severity, diagnostic accuracy of screening tools, and TB outcomes. Methods We enrolled and followed adults with HIV (CD4 ≤ 350 cells/µL) initiating antiretroviral therapy (ART) at two HIV/AIDS clinics in Uganda from July 2013 to December 2016. All participants underwent TB screening and sputum collection for TB testing (Xpert MTB/RIF [Xpert], culture). We evaluated diagnostic accuracy of four WHO-recommended TB screening strategies (symptom screen; C-reactive protiein [CRP]; symptom screen followed by CRP, if symptomatic [symptoms + CRP]; Xpert) for culture-positive TB and compared TB prevalence, days-to-treatment initiation, and 3-month mortality by sex. Results Of 1,549 participants, 727 (46.9%) were male and 236 (15.2%) had culture-positive TB. Compared to females, males had lower pre-ART CD4 counts (median 139 vs. 183 cells/µL, p < 0.001), higher TB prevalence (20.5% vs. 10.6%, p < 0.001), and higher mycobacterial load as measured by Xpert semi-quantitative grade (p = 0.03). Sensitivity was high (≥ 89.8%) for all screening strategies except Xpert (Xpert sensitivity 57.2%) and did not differ by sex. Specificity varied widely from 13.9% for symptom screen to 99.2% for Xpert, and was 5–15% lower for males than females for symptom screen, CRP, and symptoms + CRP. Among PWH with culture-positive TB, median days-to-treatment initiation (2 vs. 4, p = 0.13) and 3-month mortality (9.4% vs. 9.2%, p = 0.96) were similar for males and females. Conclusions Although ART-naïve males undergoing systematic screening had more advanced HIV and TB than females, days-to-TB treatment initiation and early TB mortality were similar, suggesting that systematic TB screening has the potential to reduce sex-based disparities in TB outcomes.
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spelling doaj-art-484017f5ed844862a2e91d1e077dc1022025-08-20T03:08:05ZengBMCBMC Infectious Diseases1471-23342025-04-0125111010.1186/s12879-025-10835-4Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, UgandaLelia H. Chaisson0Fred C. Semitala1Sandra Mwebe2Eileen P. Scully3Jane Katende4Lucy Asege5Martha Nakaye6Alfred O. Andama7Adithya Cattamanchi8Christina Yoon9UCSF Center for Tuberculosis, University of California, San FranciscoDepartment of Internal Medicine, Makerere UniversityInfectious Diseases Research CollaborationDivision of Infectious Diseases, Department of Medicine, Johns Hopkins University School of MedicineInfectious Diseases Research CollaborationInfectious Diseases Research CollaborationInfectious Diseases Research CollaborationDepartment of Internal Medicine, Makerere UniversityDivision of Pulmonary Diseases and Critical Care Medicine, Department of Medicine, University of California IrvineUCSF Center for Tuberculosis, University of California, San FranciscoAbstract Background Systematic tuberculosis (TB) screening is recommended for all people with HIV (PWH) because of its potential to improve TB outcomes through earlier diagnosis and treatment initiation. As such, systematic screening may be particularly important for men, who experience excess TB prevalence and mortality compared to women. We assessed sex differences among PWH undergoing systematic TB screening, including TB prevalence and severity, diagnostic accuracy of screening tools, and TB outcomes. Methods We enrolled and followed adults with HIV (CD4 ≤ 350 cells/µL) initiating antiretroviral therapy (ART) at two HIV/AIDS clinics in Uganda from July 2013 to December 2016. All participants underwent TB screening and sputum collection for TB testing (Xpert MTB/RIF [Xpert], culture). We evaluated diagnostic accuracy of four WHO-recommended TB screening strategies (symptom screen; C-reactive protiein [CRP]; symptom screen followed by CRP, if symptomatic [symptoms + CRP]; Xpert) for culture-positive TB and compared TB prevalence, days-to-treatment initiation, and 3-month mortality by sex. Results Of 1,549 participants, 727 (46.9%) were male and 236 (15.2%) had culture-positive TB. Compared to females, males had lower pre-ART CD4 counts (median 139 vs. 183 cells/µL, p < 0.001), higher TB prevalence (20.5% vs. 10.6%, p < 0.001), and higher mycobacterial load as measured by Xpert semi-quantitative grade (p = 0.03). Sensitivity was high (≥ 89.8%) for all screening strategies except Xpert (Xpert sensitivity 57.2%) and did not differ by sex. Specificity varied widely from 13.9% for symptom screen to 99.2% for Xpert, and was 5–15% lower for males than females for symptom screen, CRP, and symptoms + CRP. Among PWH with culture-positive TB, median days-to-treatment initiation (2 vs. 4, p = 0.13) and 3-month mortality (9.4% vs. 9.2%, p = 0.96) were similar for males and females. Conclusions Although ART-naïve males undergoing systematic screening had more advanced HIV and TB than females, days-to-TB treatment initiation and early TB mortality were similar, suggesting that systematic TB screening has the potential to reduce sex-based disparities in TB outcomes.https://doi.org/10.1186/s12879-025-10835-4TuberculosisHIVSex differencesSystematic screeningSymptomsC-reactive protein
spellingShingle Lelia H. Chaisson
Fred C. Semitala
Sandra Mwebe
Eileen P. Scully
Jane Katende
Lucy Asege
Martha Nakaye
Alfred O. Andama
Adithya Cattamanchi
Christina Yoon
Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
BMC Infectious Diseases
Tuberculosis
HIV
Sex differences
Systematic screening
Symptoms
C-reactive protein
title Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
title_full Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
title_fullStr Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
title_full_unstemmed Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
title_short Sex differences in systematic screening for tuberculosis among antiretroviral therapy naïve people with HIV in Kampala, Uganda
title_sort sex differences in systematic screening for tuberculosis among antiretroviral therapy naive people with hiv in kampala uganda
topic Tuberculosis
HIV
Sex differences
Systematic screening
Symptoms
C-reactive protein
url https://doi.org/10.1186/s12879-025-10835-4
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