Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans
Pathogenic yeast are resistant to the existing synthetic antifungal agents, demanding an additional efforts to seek more effective therapeutic agents as antifungal against such pathogens. In order to achieve this, we try to evaluate the antifungal activity of black seed, honey and zam-zam water usi...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Umaru Musa Yar'adua University, Katsina, Nigeria
2018-12-01
|
| Series: | UMYU Journal of Microbiology Research |
| Subjects: | |
| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/227 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145880642945024 |
|---|---|
| author | Ummasalma Aliyu Saulawa Sani Liti Hamisu |
| author_facet | Ummasalma Aliyu Saulawa Sani Liti Hamisu |
| author_sort | Ummasalma Aliyu Saulawa |
| collection | DOAJ |
| description |
Pathogenic yeast are resistant to the existing synthetic antifungal agents, demanding an additional efforts to seek more effective therapeutic agents as antifungal against such pathogens. In order to achieve this, we try to evaluate the antifungal activity of black seed, honey and zam-zam water using C. albican as the test organism. Samples of black seed powder, honey (HN) and Zam-zam water (ZZ) were collected from Katsina metropolis, Katsina state. The samples were then transported to the Microbiology laboratory, Umaru Musa Yar’adua University Katsina for analysis. An ethanolic extraction of the black seed was prepared. The clinical isolates C. albicans was cultured on Sabouraud dextrose agar (SDA). Agar well diffusion method was used in evaluating the synergistic activity of the test samples on C. albicans. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the test samples were also evaluated. The result shows that ethanolic black seed extract (BSE) has the highest zone of inhibition at 14mm followed by honey (HN) with 12mm while Zam-zam (ZZ) water having the least at 2mm. The MIC and MFC of the most active sample were found to be at 30mg/ml and 60mg/ml respectively.
|
| format | Article |
| id | doaj-art-483e9e8dbbf340e687ab3f2c63e45918 |
| institution | OA Journals |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-483e9e8dbbf340e687ab3f2c63e459182025-08-20T02:28:00ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222018-12-013210.47430/ujmr.1832.017227Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicansUmmasalma Aliyu Saulawa0Sani Liti Hamisu1Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University Katsina, Nigeria.Institute of Infection and Global Health, School of Life Science, University of Liverpool, United Kingdom. Pathogenic yeast are resistant to the existing synthetic antifungal agents, demanding an additional efforts to seek more effective therapeutic agents as antifungal against such pathogens. In order to achieve this, we try to evaluate the antifungal activity of black seed, honey and zam-zam water using C. albican as the test organism. Samples of black seed powder, honey (HN) and Zam-zam water (ZZ) were collected from Katsina metropolis, Katsina state. The samples were then transported to the Microbiology laboratory, Umaru Musa Yar’adua University Katsina for analysis. An ethanolic extraction of the black seed was prepared. The clinical isolates C. albicans was cultured on Sabouraud dextrose agar (SDA). Agar well diffusion method was used in evaluating the synergistic activity of the test samples on C. albicans. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the test samples were also evaluated. The result shows that ethanolic black seed extract (BSE) has the highest zone of inhibition at 14mm followed by honey (HN) with 12mm while Zam-zam (ZZ) water having the least at 2mm. The MIC and MFC of the most active sample were found to be at 30mg/ml and 60mg/ml respectively. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/227Black seedHoneyZam-ZamCandida albicans |
| spellingShingle | Ummasalma Aliyu Saulawa Sani Liti Hamisu Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans UMYU Journal of Microbiology Research Black seed Honey Zam-Zam Candida albicans |
| title | Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans |
| title_full | Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans |
| title_fullStr | Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans |
| title_full_unstemmed | Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans |
| title_short | Evaluation of Synergistic Activity of Black Seed Extract (Nigella sativa), Honey and Zamzam Water on Candida albicans |
| title_sort | evaluation of synergistic activity of black seed extract nigella sativa honey and zamzam water on candida albicans |
| topic | Black seed Honey Zam-Zam Candida albicans |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/227 |
| work_keys_str_mv | AT ummasalmaaliyusaulawa evaluationofsynergisticactivityofblackseedextractnigellasativahoneyandzamzamwateroncandidaalbicans AT sanilitihamisu evaluationofsynergisticactivityofblackseedextractnigellasativahoneyandzamzamwateroncandidaalbicans |