Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool
Background: Several studies reported the health benefits of Opuntia ficus indica extracts. Aim: This study aimed to (1) optimizes extraction conditions. (2) Point out its antioxidant and cytoprotective potential against cadmium toxicity in rat liver homogenate cells and HepG2. Methods: This ex...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2024-12-01
|
| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=215882 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850202886246498304 |
|---|---|
| author | Jihen Taleb Saida Ncibi Sabah Dhibi Khaled Athmouni Najet Ghouaidia Aida Dhibi Abdurraouf Zaet Lazhar Zourgui |
| author_facet | Jihen Taleb Saida Ncibi Sabah Dhibi Khaled Athmouni Najet Ghouaidia Aida Dhibi Abdurraouf Zaet Lazhar Zourgui |
| author_sort | Jihen Taleb |
| collection | DOAJ |
| description | Background:
Several studies reported the health benefits of Opuntia ficus indica extracts.
Aim:
This study aimed to (1) optimizes extraction conditions. (2) Point out its antioxidant and cytoprotective potential against cadmium toxicity in rat liver homogenate cells and HepG2.
Methods:
This experiment used the response surface methodology. It helped to determine the optimum conditions necessary to get the highest phenolic compound yield from Opuntia ficus indica cladodes aqueous extract. Then, a chromatography analysis was conducted to determine the phenolic compounds present in this extract. To explore the antioxidant abilities of this extract, this study used superoxide radical and hydroxyl radicals scavenging assays. Finally, to explore its biological benefits and its protective effect against cadmium toxicity, these research investigators carried out (1) an in vitro protein glycation test (2) MTT and inverted microscope imaging in HepG2 and HEK293 cell line (3) a lipid peroxidation test using rat erythrocytes.
Results:
The best extraction conditions were 10 minutes for the extraction time, 80°C for the temperature, and 30 ml/g for the liquid-solid ratio. The in vitro tests showed that Opuntia ficus indica aqueous extract has a high capacity to disable the free radical effect and prevent HepG2 and erythrocytes. Besides, the HPLC method identified 5 phenolic compounds within this extract.
Conclusion:
This study confirmed the health benefits of Opuntia ficus indica. Thus, more attention should be given to investigating this plant's capacities and isolating and identifying its bioactive molecules. [Open Vet J 2024; 14(12.000): 3257-3268] |
| format | Article |
| id | doaj-art-483dfcb74e10495fadb7c071d784d04f |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-483dfcb74e10495fadb7c071d784d04f2025-08-20T02:11:38ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123257326810.5455/OVJ.2024.v14.i12.11215882Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing toolJihen Taleb0Saida Ncibi1Sabah Dhibi2Khaled Athmouni3Najet Ghouaidia4Aida Dhibi5Abdurraouf Zaet6Lazhar Zourgui1 Research Lab. BMA "Biodiversities, Molecules, Applications", Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia. 2 Laboratory of Environmental Biotechnologies and Oasis Ecosystem (BEEO), 2100, Gafsa, Tunisia. 2 Laboratory of Environmental Biotechnologies and Oasis Ecosystem (BEEO), 2100, Gafsa, Tunisia. 3 Faculty of Science, Jazan University, Kingdom of Saudi Arabia. 2 Laboratory of Environmental Biotechnologies and Oasis Ecosystem (BEEO), 2100, Gafsa, Tunisia. 4 Department of life sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and planktonology, University of Sfax Tunisia, Faculty of Sciences, Unit UR 11 ES 72/Street of Soukra Km 3.5, B.P. 1171, CP 3000 Sfax, Tunisia. 1 Research Lab. BMA "Biodiversities, Molecules, Applications", Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia. 5 Faculty of medical technology, University of Zawia, Libya. 6 Biomedical research team, University of Zawia, Libya. 1 Research Lab. BMA "Biodiversities, Molecules, Applications", Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.Background: Several studies reported the health benefits of Opuntia ficus indica extracts. Aim: This study aimed to (1) optimizes extraction conditions. (2) Point out its antioxidant and cytoprotective potential against cadmium toxicity in rat liver homogenate cells and HepG2. Methods: This experiment used the response surface methodology. It helped to determine the optimum conditions necessary to get the highest phenolic compound yield from Opuntia ficus indica cladodes aqueous extract. Then, a chromatography analysis was conducted to determine the phenolic compounds present in this extract. To explore the antioxidant abilities of this extract, this study used superoxide radical and hydroxyl radicals scavenging assays. Finally, to explore its biological benefits and its protective effect against cadmium toxicity, these research investigators carried out (1) an in vitro protein glycation test (2) MTT and inverted microscope imaging in HepG2 and HEK293 cell line (3) a lipid peroxidation test using rat erythrocytes. Results: The best extraction conditions were 10 minutes for the extraction time, 80°C for the temperature, and 30 ml/g for the liquid-solid ratio. The in vitro tests showed that Opuntia ficus indica aqueous extract has a high capacity to disable the free radical effect and prevent HepG2 and erythrocytes. Besides, the HPLC method identified 5 phenolic compounds within this extract. Conclusion: This study confirmed the health benefits of Opuntia ficus indica. Thus, more attention should be given to investigating this plant's capacities and isolating and identifying its bioactive molecules. [Open Vet J 2024; 14(12.000): 3257-3268]http://www.ejmanager.com/fulltextpdf.php?mno=215882antioxidantcytoprotectiveextractionoptimizationopuntia ficus indica cladode |
| spellingShingle | Jihen Taleb Saida Ncibi Sabah Dhibi Khaled Athmouni Najet Ghouaidia Aida Dhibi Abdurraouf Zaet Lazhar Zourgui Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool Open Veterinary Journal antioxidant cytoprotective extraction optimization opuntia ficus indica cladode |
| title | Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool |
| title_full | Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool |
| title_fullStr | Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool |
| title_full_unstemmed | Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool |
| title_short | Cytoprotective potential of Opuntia ficus indica cladode extract against cadmium toxicity in HepG2 and HEK293 cell lines using surface methodology as an optimizing tool |
| title_sort | cytoprotective potential of opuntia ficus indica cladode extract against cadmium toxicity in hepg2 and hek293 cell lines using surface methodology as an optimizing tool |
| topic | antioxidant cytoprotective extraction optimization opuntia ficus indica cladode |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=215882 |
| work_keys_str_mv | AT jihentaleb cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT saidancibi cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT sabahdhibi cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT khaledathmouni cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT najetghouaidia cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT aidadhibi cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT abdurraoufzaet cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool AT lazharzourgui cytoprotectivepotentialofopuntiaficusindicacladodeextractagainstcadmiumtoxicityinhepg2andhek293celllinesusingsurfacemethodologyasanoptimizingtool |