NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.)
Background. Gray mold disease caused by Botrytis cinerea Pers. is a phytosanitary problem in blueberry (Vaccinium corymbosum L.). It is characterized for affecting soft tissues (flowers and fruits) in the field and in the post-harvest stage, reducing crop yield. This has motivated to the search for...
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Universidad Autónoma de Yucatán
2024-11-01
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| Series: | Tropical and Subtropical Agroecosystems |
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| Online Access: | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5072 |
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| author | Yasmin Carmen Arestegui Cantoral Eryka Gaslac Zumaeta Betsabe Leon Ttacca |
| author_facet | Yasmin Carmen Arestegui Cantoral Eryka Gaslac Zumaeta Betsabe Leon Ttacca |
| author_sort | Yasmin Carmen Arestegui Cantoral |
| collection | DOAJ |
| description | Background. Gray mold disease caused by Botrytis cinerea Pers. is a phytosanitary problem in blueberry (Vaccinium corymbosum L.). It is characterized for affecting soft tissues (flowers and fruits) in the field and in the post-harvest stage, reducing crop yield. This has motivated to the search for native strains of Trichoderma that are effective in biological control. Objective. To determine the effect of 19 native Trichoderma strains on the control of gray mold on flowers and fruits of blueberry under laboratory conditions. Methodology. Under a complete randomized experimental design, mycoparasitism and antibiosis tests were carried out to evaluate colonization and mycelial inhibition of the pathogen, respectively, using the dual confrontation method and production of secondary metabolites. For the bioassays, a suspension of 1×106 conidia mL-1 per strain was sprayed on blueberry flowers and fruits. Disease incidence and severity were evaluated. Data were analyzed with the statistical program InfoStat. Results. All Trichoderma strains completely colonized the pathogen, being considered as aggressive mycoparasites and inhibited the mycelial growth of the pathogen up to 42.74%. Likewise, the application of Trichoderma spore suspension had inhibitory effects on gray mold, highlighting the strain HE- ArT161 that reduced the incidence (66.67% and 20%) and severity (34% and 12%), respectively in flowers and fruits. Implications. The use of these native strains of Trichoderma may contribute to future research in the field where they can be incorporated for an integrated management of the disease. Conclusion. These Trichoderma isolates against B. cinerea showed in vitro control, stimulating mycelial inhibition of the pathogen and reducing the development of disease lesions. |
| format | Article |
| id | doaj-art-48371fa6196346c483abfb7cf47ced44 |
| institution | OA Journals |
| issn | 1870-0462 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universidad Autónoma de Yucatán |
| record_format | Article |
| series | Tropical and Subtropical Agroecosystems |
| spelling | doaj-art-48371fa6196346c483abfb7cf47ced442025-08-20T02:16:46ZengUniversidad Autónoma de YucatánTropical and Subtropical Agroecosystems1870-04622024-11-0127310.56369/tsaes.50721743NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.)Yasmin Carmen Arestegui Cantoral0Eryka Gaslac Zumaeta1Betsabe Leon Ttacca2"Universidad Nacional de Cañete""Grupo de Investigación para el Desarrollo Sustentable de Ceja de Selva, Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas""Universidad Nacional de Cañete"Background. Gray mold disease caused by Botrytis cinerea Pers. is a phytosanitary problem in blueberry (Vaccinium corymbosum L.). It is characterized for affecting soft tissues (flowers and fruits) in the field and in the post-harvest stage, reducing crop yield. This has motivated to the search for native strains of Trichoderma that are effective in biological control. Objective. To determine the effect of 19 native Trichoderma strains on the control of gray mold on flowers and fruits of blueberry under laboratory conditions. Methodology. Under a complete randomized experimental design, mycoparasitism and antibiosis tests were carried out to evaluate colonization and mycelial inhibition of the pathogen, respectively, using the dual confrontation method and production of secondary metabolites. For the bioassays, a suspension of 1×106 conidia mL-1 per strain was sprayed on blueberry flowers and fruits. Disease incidence and severity were evaluated. Data were analyzed with the statistical program InfoStat. Results. All Trichoderma strains completely colonized the pathogen, being considered as aggressive mycoparasites and inhibited the mycelial growth of the pathogen up to 42.74%. Likewise, the application of Trichoderma spore suspension had inhibitory effects on gray mold, highlighting the strain HE- ArT161 that reduced the incidence (66.67% and 20%) and severity (34% and 12%), respectively in flowers and fruits. Implications. The use of these native strains of Trichoderma may contribute to future research in the field where they can be incorporated for an integrated management of the disease. Conclusion. These Trichoderma isolates against B. cinerea showed in vitro control, stimulating mycelial inhibition of the pathogen and reducing the development of disease lesions.https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5072antibiosisbiocontrolcrecimiento micelialmicoparasitismopatógeno. |
| spellingShingle | Yasmin Carmen Arestegui Cantoral Eryka Gaslac Zumaeta Betsabe Leon Ttacca NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) Tropical and Subtropical Agroecosystems antibiosis biocontrol crecimiento micelial micoparasitismo patógeno. |
| title | NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) |
| title_full | NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) |
| title_fullStr | NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) |
| title_full_unstemmed | NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) |
| title_short | NATIVE Trichoderma STRAINS FOR THE CONTROL OF GREY MOULD (Botrytis cinerea Pers.) IN FRUITS AND FLOWERS OF BLUEBERRY (Vaccinium corymbosum L.) |
| title_sort | native trichoderma strains for the control of grey mould botrytis cinerea pers in fruits and flowers of blueberry vaccinium corymbosum l |
| topic | antibiosis biocontrol crecimiento micelial micoparasitismo patógeno. |
| url | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5072 |
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