Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production

Abstract The CRISPR-Cas9 system has frequently been used for genome editing in Streptomyces; however, cytotoxicity, caused by off-target cleavage, limits its application. In this study, we implement innovative modification to Cas9, strategically addressing challenges encountered during gene manipula...

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Bibliographic Details
Main Authors: Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-56278-y
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