Interlaboratory validation of an optimized protocol for measuring α-amylase activity by the INFOGEST international research network

Interlaboratory validation of an optimized protocol for measuring α-amylase activity by the INFOGEST international research network

Abstract The activity of α-amylases is frequently determined using a single-point assay at 20 °C. Previous work within INFOGEST “Working Group 5 - Starch digestion and amylases” identified significant interlaboratory variation with this protocol. The current study aimed to evaluate the repeatability...

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Main Authors: Daniela Freitas, Shannon Gwala, Gwénaële Henry, Athina Lazaridou, Christine Boesch, Dorine Duijsens, Faye Wheller, Ivan M. Lopez-Rodulfo, Kali Kotsiou, Kendall R. Corbin, Marilisa Alongi, Mario M. Martinez, Maryam S. Hafiz, Monic M. M. Tomassen, Natalia Perez-Moral, Natalia P. Vidal, Renata M. C. Ariëns, Sebnem Simsek, Sedef Nehir El, Sibel Karakaya, Steven Le Feunteun, Shanna Bastiaan-Net, Svenja Krause, Bin Zhang, Caroline Orfila, Simon Ballance, Terri Grassby
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-12561-y
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