Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i>
<i>Sanghuangporous vaninii</i>, as an edible and medicinal macrofungus, represents a high source of polyphenols with considerable antioxidant activities. However, due to the significant differences in polyphenol content and bioactivity caused by different cultivation substrates, its anti...
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2024-12-01
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| Series: | Antioxidants |
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| author | Peng Liu Yuyang Wang Daoyou Chen Zhengpeng Li Di Wu Zhong Zhang Wanchao Chen Wen Li Yan Yang |
| author_facet | Peng Liu Yuyang Wang Daoyou Chen Zhengpeng Li Di Wu Zhong Zhang Wanchao Chen Wen Li Yan Yang |
| author_sort | Peng Liu |
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| description | <i>Sanghuangporous vaninii</i>, as an edible and medicinal macrofungus, represents a high source of polyphenols with considerable antioxidant activities. However, due to the significant differences in polyphenol content and bioactivity caused by different cultivation substrates, its antioxidant mechanism has not been fully determined. In this paper, five groups of <i>S. vaninii</i> fruiting bodies were collected from cultivation substrates from different areas. The ethanol extracts of mulberry sawdust from Haining City (HNMS) had the highest polyphenol content, as well as excellent antioxidant activity. HNMS3, a polyphenol component with promising antioxidant capacity, was further isolated through optimization with different extractants, silica gel column chromatography, and thin layer chromatography analysis. UPLC-Q-TOF-MS analysis showed that HNMS3 was composed of 33 compounds, corresponding to 257 targets of oxidative stress by network pharmacology analysis, which were strongly associated with mental health and neurodegenerative diseases. Protein–protein interaction and molecular docking analysis indicated that eight hub genes (PPARG, IL-6, STAT3, PTGS2, SRC, MTOR, ERS1, and EGFR) are attributed to the regulation of the key compounds hispidin, inoscavin A, inoscavin_C, and phellibaumin B. Consequently, this study obtains <i>S. vaninii</i> polyphenolic component HNMS3 with excellent antioxidant capacity, simultaneously revealing its potential antioxidant mechanisms, providing new insights into the application of <i>S. vaninii</i>. |
| format | Article |
| id | doaj-art-48140f030af74f9b8ec651b28cddfe9e |
| institution | OA Journals |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-48140f030af74f9b8ec651b28cddfe9e2025-08-20T02:01:01ZengMDPI AGAntioxidants2076-39212024-12-011312148710.3390/antiox13121487Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i>Peng Liu0Yuyang Wang1Daoyou Chen2Zhengpeng Li3Di Wu4Zhong Zhang5Wanchao Chen6Wen Li7Yan Yang8Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaShanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China<i>Sanghuangporous vaninii</i>, as an edible and medicinal macrofungus, represents a high source of polyphenols with considerable antioxidant activities. However, due to the significant differences in polyphenol content and bioactivity caused by different cultivation substrates, its antioxidant mechanism has not been fully determined. In this paper, five groups of <i>S. vaninii</i> fruiting bodies were collected from cultivation substrates from different areas. The ethanol extracts of mulberry sawdust from Haining City (HNMS) had the highest polyphenol content, as well as excellent antioxidant activity. HNMS3, a polyphenol component with promising antioxidant capacity, was further isolated through optimization with different extractants, silica gel column chromatography, and thin layer chromatography analysis. UPLC-Q-TOF-MS analysis showed that HNMS3 was composed of 33 compounds, corresponding to 257 targets of oxidative stress by network pharmacology analysis, which were strongly associated with mental health and neurodegenerative diseases. Protein–protein interaction and molecular docking analysis indicated that eight hub genes (PPARG, IL-6, STAT3, PTGS2, SRC, MTOR, ERS1, and EGFR) are attributed to the regulation of the key compounds hispidin, inoscavin A, inoscavin_C, and phellibaumin B. Consequently, this study obtains <i>S. vaninii</i> polyphenolic component HNMS3 with excellent antioxidant capacity, simultaneously revealing its potential antioxidant mechanisms, providing new insights into the application of <i>S. vaninii</i>.https://www.mdpi.com/2076-3921/13/12/1487<i>Sanghuangporous vaninii</i>polyphenolsantioxidant activityisolationnetwork pharmacology |
| spellingShingle | Peng Liu Yuyang Wang Daoyou Chen Zhengpeng Li Di Wu Zhong Zhang Wanchao Chen Wen Li Yan Yang Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> Antioxidants <i>Sanghuangporous vaninii</i> polyphenols antioxidant activity isolation network pharmacology |
| title | Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> |
| title_full | Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> |
| title_fullStr | Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> |
| title_full_unstemmed | Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> |
| title_short | Isolation and Antioxidant Mechanism of Polyphenols from <i>Sanghuangporous vaninii</i> |
| title_sort | isolation and antioxidant mechanism of polyphenols from i sanghuangporous vaninii i |
| topic | <i>Sanghuangporous vaninii</i> polyphenols antioxidant activity isolation network pharmacology |
| url | https://www.mdpi.com/2076-3921/13/12/1487 |
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