eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection
In this study, we revealed a critical role of eukaryotic elongation factor-2 kinase (eEF-2K), a negative regulator of protein synthesis, in regulating T cells during vaccinia virus (VACV) infection. We found that eEF-2K-deficient (eEF-2K⁻/⁻) mice exhibited a significantly higher proportion of VACV-s...
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MDPI AG
2024-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/1/26 |
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| author | Liqing Wang Benny Shone Song Rayansh Poojary Xiaofang Xiong Xingcong Ren Jin-Ming Yang Jianxun Song |
| author_facet | Liqing Wang Benny Shone Song Rayansh Poojary Xiaofang Xiong Xingcong Ren Jin-Ming Yang Jianxun Song |
| author_sort | Liqing Wang |
| collection | DOAJ |
| description | In this study, we revealed a critical role of eukaryotic elongation factor-2 kinase (eEF-2K), a negative regulator of protein synthesis, in regulating T cells during vaccinia virus (VACV) infection. We found that eEF-2K-deficient (eEF-2K⁻/⁻) mice exhibited a significantly higher proportion of VACV-specific effector CD8<sup>+</sup> T cells without compromising the development of VACV-specific memory CD8<sup>+</sup> T cells. RNA sequencing demonstrated that eEF-2K⁻/⁻ VACV-specific effector CD8<sup>+</sup> T cells had enhanced functionality, which improves their capacity to combat viral infection during the effector phase. Moreover, we identified tumor necrosis factor receptor-associated factor 3 (TRAF3) as a critical mediator of the stronger antiviral response observed in eEF-2K⁻/⁻ effector CD8<sup>+</sup> T cells. These findings suggest that targeting eEF-2K may provide a novel strategy to augmenting effector CD8<sup>+</sup> T cell responses against viral infections. |
| format | Article |
| id | doaj-art-4806b76ea3a04db5972cd4896997de2e |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-4806b76ea3a04db5972cd4896997de2e2025-01-24T13:52:18ZengMDPI AGViruses1999-49152024-12-011712610.3390/v17010026eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral InfectionLiqing Wang0Benny Shone Song1Rayansh Poojary2Xiaofang Xiong3Xingcong Ren4Jin-Ming Yang5Jianxun Song6Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USADepartment of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USADepartment of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USADepartment of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USADepartment of Toxicology and Cancer Biology, University of Kentucky College of Medicine, Lexington, KY 40536, USADepartment of Toxicology and Cancer Biology, University of Kentucky College of Medicine, Lexington, KY 40536, USADepartment of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, Bryan, TX 77807, USAIn this study, we revealed a critical role of eukaryotic elongation factor-2 kinase (eEF-2K), a negative regulator of protein synthesis, in regulating T cells during vaccinia virus (VACV) infection. We found that eEF-2K-deficient (eEF-2K⁻/⁻) mice exhibited a significantly higher proportion of VACV-specific effector CD8<sup>+</sup> T cells without compromising the development of VACV-specific memory CD8<sup>+</sup> T cells. RNA sequencing demonstrated that eEF-2K⁻/⁻ VACV-specific effector CD8<sup>+</sup> T cells had enhanced functionality, which improves their capacity to combat viral infection during the effector phase. Moreover, we identified tumor necrosis factor receptor-associated factor 3 (TRAF3) as a critical mediator of the stronger antiviral response observed in eEF-2K⁻/⁻ effector CD8<sup>+</sup> T cells. These findings suggest that targeting eEF-2K may provide a novel strategy to augmenting effector CD8<sup>+</sup> T cell responses against viral infections.https://www.mdpi.com/1999-4915/17/1/26eEF-2Keffector CD8<sup>+</sup> T cellsvaccinia virus (VACV)T cell immunityTRAF3viral infection |
| spellingShingle | Liqing Wang Benny Shone Song Rayansh Poojary Xiaofang Xiong Xingcong Ren Jin-Ming Yang Jianxun Song eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection Viruses eEF-2K effector CD8<sup>+</sup> T cells vaccinia virus (VACV) T cell immunity TRAF3 viral infection |
| title | eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection |
| title_full | eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection |
| title_fullStr | eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection |
| title_full_unstemmed | eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection |
| title_short | eEF-2K Deficiency Boosts the Virus-Specific Effector CD8<sup>+</sup> T Cell Responses During Viral Infection |
| title_sort | eef 2k deficiency boosts the virus specific effector cd8 sup sup t cell responses during viral infection |
| topic | eEF-2K effector CD8<sup>+</sup> T cells vaccinia virus (VACV) T cell immunity TRAF3 viral infection |
| url | https://www.mdpi.com/1999-4915/17/1/26 |
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