Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer
BackgroundColorectal cancer (CRC) is a leading cause of morbidity and mortality, and timely diagnosis and intervention are crucial for cancer patients. Transfer RNA-derived fragments (tRFs) play a noncoding regulatory role in organisms. Serum EV(extracellular vesicles), as an integral mediator of in...
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Frontiers Media S.A.
2024-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1474095/full |
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| author | Jiefei Peng Jiefei Peng Fan Bu Fan Bu Lei Duan Lei Duan Anna Song Guojun Wang Zhijun Zhang Zhijun Zhang |
| author_facet | Jiefei Peng Jiefei Peng Fan Bu Fan Bu Lei Duan Lei Duan Anna Song Guojun Wang Zhijun Zhang Zhijun Zhang |
| author_sort | Jiefei Peng |
| collection | DOAJ |
| description | BackgroundColorectal cancer (CRC) is a leading cause of morbidity and mortality, and timely diagnosis and intervention are crucial for cancer patients. Transfer RNA-derived fragments (tRFs) play a noncoding regulatory role in organisms. Serum EV(extracellular vesicles), as an integral mediator of intercellular transmission of genetic information vesicles in Transfer RNA-derived fragment (tRF RNA), are expected to be minimally invasive diagnostic and predictive biologic factors of CRC.MethodsCollect serum samples from 205 CRC patients, and then isolate extracellular vesicles from the serum. Captured the physical morphology of EV through transmission electron microscopy. The particle size was detected by particle size assay, and protein expression on the surface of EV was verified by Western blot. Gene microarrays were screened for differentially expressed tRF-RNA. TRF RNAs were verified by qPCR for differential expression in 205 CRC patients and 201 healthy donors, assessing the CRC diagnostic efficiency by area under the curve (AUC).ResultsCompared with 201 healthy donors, CRC patients experienced significantly down-regulated serum EV 3’tRF-ThrCGT while significantly up-regulated 3’tRF-mtlleGAT. Serum EV 3’tRF-ThrCGT and 3’tRF-mtlleGAT predictive diagnostic efficiency: 0.669 and 0.656, and the combination of CEA and CA724 predictive diagnostic efficiency was 0.938.ConclusionThe study data showed that 3’tRF-ThrCGT and 3’tRF-mtlelGAT can be minimally invasive diagnostic CRC indicators. The combination of tumor markers CEA and CA724 has important diagnostic significance. |
| format | Article |
| id | doaj-art-4805f703ff744c7eac01fa1daa1ba520 |
| institution | OA Journals |
| issn | 2234-943X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-4805f703ff744c7eac01fa1daa1ba5202025-08-20T02:10:31ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-10-011410.3389/fonc.2024.14740951474095Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancerJiefei Peng0Jiefei Peng1Fan Bu2Fan Bu3Lei Duan4Lei Duan5Anna Song6Guojun Wang7Zhijun Zhang8Zhijun Zhang9Department of Clinical Laboratory, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaShandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaDepartment of Clinical Laboratory, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaShandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaDepartment of Clinical Laboratory, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaShandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaDepartment of Reproduction and Genetics, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaDepartment of Neurosurgery, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaDepartment of Clinical Laboratory, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaShandong Provincial Key Medical and Health Laboratory of Anti-Drug Resistant Drug Research, Affiliated Taian City Central Hospital of Qingdao University, Taian, ChinaBackgroundColorectal cancer (CRC) is a leading cause of morbidity and mortality, and timely diagnosis and intervention are crucial for cancer patients. Transfer RNA-derived fragments (tRFs) play a noncoding regulatory role in organisms. Serum EV(extracellular vesicles), as an integral mediator of intercellular transmission of genetic information vesicles in Transfer RNA-derived fragment (tRF RNA), are expected to be minimally invasive diagnostic and predictive biologic factors of CRC.MethodsCollect serum samples from 205 CRC patients, and then isolate extracellular vesicles from the serum. Captured the physical morphology of EV through transmission electron microscopy. The particle size was detected by particle size assay, and protein expression on the surface of EV was verified by Western blot. Gene microarrays were screened for differentially expressed tRF-RNA. TRF RNAs were verified by qPCR for differential expression in 205 CRC patients and 201 healthy donors, assessing the CRC diagnostic efficiency by area under the curve (AUC).ResultsCompared with 201 healthy donors, CRC patients experienced significantly down-regulated serum EV 3’tRF-ThrCGT while significantly up-regulated 3’tRF-mtlleGAT. Serum EV 3’tRF-ThrCGT and 3’tRF-mtlleGAT predictive diagnostic efficiency: 0.669 and 0.656, and the combination of CEA and CA724 predictive diagnostic efficiency was 0.938.ConclusionThe study data showed that 3’tRF-ThrCGT and 3’tRF-mtlelGAT can be minimally invasive diagnostic CRC indicators. The combination of tumor markers CEA and CA724 has important diagnostic significance.https://www.frontiersin.org/articles/10.3389/fonc.2024.1474095/fullcolorectal cancerserum EV3’tRF-ThrCGT3’tRF-mtlleGATminimally invasive diagnostic predictorstumor markers |
| spellingShingle | Jiefei Peng Jiefei Peng Fan Bu Fan Bu Lei Duan Lei Duan Anna Song Guojun Wang Zhijun Zhang Zhijun Zhang Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer Frontiers in Oncology colorectal cancer serum EV 3’tRF-ThrCGT 3’tRF-mtlleGAT minimally invasive diagnostic predictors tumor markers |
| title | Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| title_full | Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| title_fullStr | Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| title_full_unstemmed | Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| title_short | Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| title_sort | serum extracellular vesicles 3 trf thrcgtand 3 trf mtllegat combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer |
| topic | colorectal cancer serum EV 3’tRF-ThrCGT 3’tRF-mtlleGAT minimally invasive diagnostic predictors tumor markers |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1474095/full |
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