Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi)
Wild tulips in Xinjiang Uygur Autonomous Region are unique and elite germplasm resources, which are widely distributed in China. It is of great necessity to protect, develop, and fully utilize those valuable materials for species diversity and breeding regimes mainly due to the destruction of primit...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2017-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.11.041 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849419293948641280 |
|---|---|
| author | LI Yue WU Yun ELMONGY Mohamed LÜ Xuesi REN Ziming SUN Minyi XIA Yiping |
| author_facet | LI Yue WU Yun ELMONGY Mohamed LÜ Xuesi REN Ziming SUN Minyi XIA Yiping |
| author_sort | LI Yue |
| collection | DOAJ |
| description | Wild tulips in Xinjiang Uygur Autonomous Region are unique and elite germplasm resources, which are widely distributed in China. It is of great necessity to protect, develop, and fully utilize those valuable materials for species diversity and breeding regimes mainly due to the destruction of primitive habitat. These wild species are often characterized numerous outstanding horticultural traits (e.g., resistance and ornamental values) with great potential in breeding. Due to changeable climatic conditions, as well as the influence of diseases, it still has many problems in overcoming the degeneration of bulbs throughout the hot summer in some regions like Zhejiang Province. Tissue culture is an effective alternative method to preserve germplasm resources compared with the conventional ways. However, the survival rate of plantlets after transplanting is much higher than the bulblets formed in vitro. Therefore, tulip bulblets in vitro obtained through the sterile sowing technology are easier to achieve the successful conservation and propagation of wild tulip under non-primitive habitat. It is of great importance for the preservation and utilization of wild tulips with excellent traits in China.The study aims to screen out the optimal conditions for the seed germination of Tulipa sinkiangensi native to Xinjiang, including sterilization method (ethanol, 2% NaClO or 0.1% HgCl<sub>2</sub>), peeling or not, gibberellin (GA<sub>3</sub>) pretreatment concentration (0, 300, 600 mg/L), basal medium (MS, 1/2MS) and culture temperature (4 ℃, 25 ℃).The results showed that 75% ethanol treatment for 30 seconds and 2% sodium hypochlorite for 10 minutes were the best sterilization method for tulip seeds, with the contamination rate of 0%. The germination rate was higher at 4 ℃ without peeling off the seed coat, while it was higher at 25 ℃ with peeling off seed coat. Four degrees celsius was necessary for breaking dormancy, whereas 25 ℃ was more suitable for seed germination and growth. The optimal conditions for seed germination of wild tulips were pretreatment with 600 mg/L GA<sub>3</sub> for 24 hours before sowing, and 1/2MS basal medium at 25 ℃. Under these conditions, the germination rate of wild tulips reached 96.67% with strong growth potential. We also found that peeling off the seed coat could not significantly increase the germination rate, but could significantly shorten the seed germination time, resulting in more robust shoot growth. Eventually, about more than 80% sprouted seeds formed small bulblets in vitro, while the seeds without peeling off the seed coat failed. Meanwhile, the formation rate of the bulblets in the aseptic sowing was significantly higher than that in the soil sowing.This study provides an experimental basis for in vitro conservation of wild tulips and helps to enhance the protection and utilization of wild tulip resources in China. |
| format | Article |
| id | doaj-art-47f760ca055040a3bced84276bdb50f0 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-47f760ca055040a3bced84276bdb50f02025-08-20T03:32:09ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552017-05-014330731610.3785/j.issn.1008-9209.2016.11.04110089209Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi)LI YueWU YunELMONGY MohamedLÜ XuesiREN ZimingSUN MinyiXIA YipingWild tulips in Xinjiang Uygur Autonomous Region are unique and elite germplasm resources, which are widely distributed in China. It is of great necessity to protect, develop, and fully utilize those valuable materials for species diversity and breeding regimes mainly due to the destruction of primitive habitat. These wild species are often characterized numerous outstanding horticultural traits (e.g., resistance and ornamental values) with great potential in breeding. Due to changeable climatic conditions, as well as the influence of diseases, it still has many problems in overcoming the degeneration of bulbs throughout the hot summer in some regions like Zhejiang Province. Tissue culture is an effective alternative method to preserve germplasm resources compared with the conventional ways. However, the survival rate of plantlets after transplanting is much higher than the bulblets formed in vitro. Therefore, tulip bulblets in vitro obtained through the sterile sowing technology are easier to achieve the successful conservation and propagation of wild tulip under non-primitive habitat. It is of great importance for the preservation and utilization of wild tulips with excellent traits in China.The study aims to screen out the optimal conditions for the seed germination of Tulipa sinkiangensi native to Xinjiang, including sterilization method (ethanol, 2% NaClO or 0.1% HgCl<sub>2</sub>), peeling or not, gibberellin (GA<sub>3</sub>) pretreatment concentration (0, 300, 600 mg/L), basal medium (MS, 1/2MS) and culture temperature (4 ℃, 25 ℃).The results showed that 75% ethanol treatment for 30 seconds and 2% sodium hypochlorite for 10 minutes were the best sterilization method for tulip seeds, with the contamination rate of 0%. The germination rate was higher at 4 ℃ without peeling off the seed coat, while it was higher at 25 ℃ with peeling off seed coat. Four degrees celsius was necessary for breaking dormancy, whereas 25 ℃ was more suitable for seed germination and growth. The optimal conditions for seed germination of wild tulips were pretreatment with 600 mg/L GA<sub>3</sub> for 24 hours before sowing, and 1/2MS basal medium at 25 ℃. Under these conditions, the germination rate of wild tulips reached 96.67% with strong growth potential. We also found that peeling off the seed coat could not significantly increase the germination rate, but could significantly shorten the seed germination time, resulting in more robust shoot growth. Eventually, about more than 80% sprouted seeds formed small bulblets in vitro, while the seeds without peeling off the seed coat failed. Meanwhile, the formation rate of the bulblets in the aseptic sowing was significantly higher than that in the soil sowing.This study provides an experimental basis for in vitro conservation of wild tulips and helps to enhance the protection and utilization of wild tulip resources in China.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.11.041wild tulip (<italic>Tulipa sinkiangensi</italic>)sterile sowingresource preservationgermination ratebulblet formation |
| spellingShingle | LI Yue WU Yun ELMONGY Mohamed LÜ Xuesi REN Ziming SUN Minyi XIA Yiping Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) 浙江大学学报. 农业与生命科学版 wild tulip (<italic>Tulipa sinkiangensi</italic>) sterile sowing resource preservation germination rate bulblet formation |
| title | Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) |
| title_full | Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) |
| title_fullStr | Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) |
| title_full_unstemmed | Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) |
| title_short | Aseptic germination and bulblet formation of wild tulip (Tulipa sinkiangensi) |
| title_sort | aseptic germination and bulblet formation of wild tulip tulipa sinkiangensi |
| topic | wild tulip (<italic>Tulipa sinkiangensi</italic>) sterile sowing resource preservation germination rate bulblet formation |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.11.041 |
| work_keys_str_mv | AT liyue asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT wuyun asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT elmongymohamed asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT luxuesi asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT renziming asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT sunminyi asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi AT xiayiping asepticgerminationandbulbletformationofwildtuliptulipasinkiangensi |