Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast
The human Thyroid Cancer-1 (hTC-1) protein, also known as C8orf4 was initially identified as a gene that was up-regulated in human thyroid cancer. Here we show that hTC-1 is a peptide that prevents the effects of over-expressing Bax in yeast. Analysis of the 106 residues of hTC-1 in available...
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Shared Science Publishers OG
2015-07-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/human-thyroid-cancer-1-tc-1-is-a-vertebrate-specific-oncogenic-protein-that-protects-against-copper-and-pro-apoptotic-genes-in-yeast/ |
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| author | Natalie K. Jones Nagla T.T. Arab Rawan Eid Nada Gharib Sara Sheiban Hojatollah Vali Chamel Khoury Alistair Murray Eric Boucher Craig A. Mandato Paul G. Young Michael T. Greenwood |
| author_facet | Natalie K. Jones Nagla T.T. Arab Rawan Eid Nada Gharib Sara Sheiban Hojatollah Vali Chamel Khoury Alistair Murray Eric Boucher Craig A. Mandato Paul G. Young Michael T. Greenwood |
| author_sort | Natalie K. Jones |
| collection | DOAJ |
| description | The human Thyroid Cancer-1 (hTC-1) protein, also known as C8orf4 was initially identified as a gene that was up-regulated in human thyroid cancer. Here we show that hTC-1 is a peptide that prevents the effects of over-expressing Bax in yeast. Analysis of the 106 residues of hTC-1 in available protein databases revealed direct orthologues in jawed-vertebrates, including mammals, frogs, fish and sharks. No TC-1 orthologue was detected in lower organisms, including yeast. Here we show that TC-1 is a general pro-survival peptide since it prevents the growth- and cell death-inducing effects of copper in yeast. Human TC-1 also prevented the deleterious effects that occur due to the over-expression of a number of key pro-apoptotic peptides, including YCA1, YBH3, NUC1, and AIF1. Even though the protective effects were more pronounced with the over-expression of YBH3 and YCA1, hTC-1 could still protect yeast mutants lacking YBH3 and YCA1 from the effects of copper sulfate. This suggests that the protective effects of TC-1 are not limited to specific pathways or processes. Taken together, our results indicate that hTC-1 is a pro-survival protein that retains its function when heterologously expressed in yeast. Thus yeast is a useful model to characterize the potential roles in cell death and survival of cancer related genes. |
| format | Article |
| id | doaj-art-47f042867bfa408ba5fcd07f9692b507 |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-47f042867bfa408ba5fcd07f9692b5072025-08-20T02:52:37ZengShared Science Publishers OGMicrobial Cell2311-26382015-07-012724725510.15698/mic2015.07.213123455678Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeastNatalie K. Jones0Nagla T.T. Arab1Rawan Eid2Nada Gharib3Sara Sheiban4Hojatollah Vali5Chamel Khoury6Alistair Murray7Eric Boucher8Craig A. Mandato9Paul G. Young10Michael T. Greenwood11Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada.Department of Biology, Queen’s University, Kingston, Ontario, Canada.Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.The human Thyroid Cancer-1 (hTC-1) protein, also known as C8orf4 was initially identified as a gene that was up-regulated in human thyroid cancer. Here we show that hTC-1 is a peptide that prevents the effects of over-expressing Bax in yeast. Analysis of the 106 residues of hTC-1 in available protein databases revealed direct orthologues in jawed-vertebrates, including mammals, frogs, fish and sharks. No TC-1 orthologue was detected in lower organisms, including yeast. Here we show that TC-1 is a general pro-survival peptide since it prevents the growth- and cell death-inducing effects of copper in yeast. Human TC-1 also prevented the deleterious effects that occur due to the over-expression of a number of key pro-apoptotic peptides, including YCA1, YBH3, NUC1, and AIF1. Even though the protective effects were more pronounced with the over-expression of YBH3 and YCA1, hTC-1 could still protect yeast mutants lacking YBH3 and YCA1 from the effects of copper sulfate. This suggests that the protective effects of TC-1 are not limited to specific pathways or processes. Taken together, our results indicate that hTC-1 is a pro-survival protein that retains its function when heterologously expressed in yeast. Thus yeast is a useful model to characterize the potential roles in cell death and survival of cancer related genes.http://microbialcell.com/researcharticles/human-thyroid-cancer-1-tc-1-is-a-vertebrate-specific-oncogenic-protein-that-protects-against-copper-and-pro-apoptotic-genes-in-yeast/TC-1apoptosiscell deathanti-apoptoticcell survivalcanceryeast |
| spellingShingle | Natalie K. Jones Nagla T.T. Arab Rawan Eid Nada Gharib Sara Sheiban Hojatollah Vali Chamel Khoury Alistair Murray Eric Boucher Craig A. Mandato Paul G. Young Michael T. Greenwood Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast Microbial Cell TC-1 apoptosis cell death anti-apoptotic cell survival cancer yeast |
| title | Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast |
| title_full | Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast |
| title_fullStr | Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast |
| title_full_unstemmed | Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast |
| title_short | Human Thyroid Cancer-1 (TC-1) is a vertebrate specific oncogenic protein that protects against copper and pro-apoptotic genes in yeast |
| title_sort | human thyroid cancer 1 tc 1 is a vertebrate specific oncogenic protein that protects against copper and pro apoptotic genes in yeast |
| topic | TC-1 apoptosis cell death anti-apoptotic cell survival cancer yeast |
| url | http://microbialcell.com/researcharticles/human-thyroid-cancer-1-tc-1-is-a-vertebrate-specific-oncogenic-protein-that-protects-against-copper-and-pro-apoptotic-genes-in-yeast/ |
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