Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa
The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (S...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2015/786010 |
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| author | Feng-Hua Chen Ke Li Lu Yin Chun-Qiu Chen Zhao-Wen Yan Gui-Ming Chen |
| author_facet | Feng-Hua Chen Ke Li Lu Yin Chun-Qiu Chen Zhao-Wen Yan Gui-Ming Chen |
| author_sort | Feng-Hua Chen |
| collection | DOAJ |
| description | The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reported in vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na+-K+-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine. |
| format | Article |
| id | doaj-art-47e90a65819e4efe8c98c3897bfc33d6 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-47e90a65819e4efe8c98c3897bfc33d62025-08-20T03:36:07ZengWileyBiochemistry Research International2090-22472090-22552015-01-01201510.1155/2015/786010786010Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted MucosaFeng-Hua Chen0Ke Li1Lu Yin2Chun-Qiu Chen3Zhao-Wen Yan4Gui-Ming Chen5Ultrasound Department, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, ChinaDepartment of General Surgery, Shanghai Jiaotong University Affiliated First People’s Hospital, Shanghai 200080, ChinaShanghai Institute of Digestive Surgery, Shanghai Jiaotong University Medical College, Shanghai 200025, ChinaDepartment of General Surgery, Shanghai Jiaotong University Affiliated First People’s Hospital, Shanghai 200080, ChinaDepartment of Pathology, Shanghai Jiaotong University Medical College, Shanghai 200025, ChinaDepartment of General Surgery, Shanghai Jiaotong University Affiliated First People’s Hospital, Shanghai 200080, ChinaThe intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reported in vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na+-K+-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.http://dx.doi.org/10.1155/2015/786010 |
| spellingShingle | Feng-Hua Chen Ke Li Lu Yin Chun-Qiu Chen Zhao-Wen Yan Gui-Ming Chen Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa Biochemistry Research International |
| title | Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa |
| title_full | Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa |
| title_fullStr | Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa |
| title_full_unstemmed | Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa |
| title_short | Protective Effect of Sodium Nitroprusside on the Rat Small Intestine Transplanted Mucosa |
| title_sort | protective effect of sodium nitroprusside on the rat small intestine transplanted mucosa |
| url | http://dx.doi.org/10.1155/2015/786010 |
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