Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum
Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/829076 |
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| author | Sohail Ahmad Shabir Ahmad Ahtaram Bibi Muhammad Saqib Ishaq Muhammad Siddique Afridi Farina Kanwal Muhammad Zakir Farid Fatima |
| author_facet | Sohail Ahmad Shabir Ahmad Ahtaram Bibi Muhammad Saqib Ishaq Muhammad Siddique Afridi Farina Kanwal Muhammad Zakir Farid Fatima |
| author_sort | Sohail Ahmad |
| collection | DOAJ |
| description | Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000 mg/mL) and then several dilutions (50, 100, 150, 200, and 250 mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard. The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated to mass spectrometer was used. The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model. |
| format | Article |
| id | doaj-art-47df543f16bb427bbc33c9dc24180c9c |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-47df543f16bb427bbc33c9dc24180c9c2025-08-20T03:36:07ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/829076829076Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferumSohail Ahmad0Shabir Ahmad1Ahtaram Bibi2Muhammad Saqib Ishaq3Muhammad Siddique Afridi4Farina Kanwal5Muhammad Zakir6Farid Fatima7Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Chemistry, Islamia College University, Peshawar 25120, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Microbiology, Abasyn University, Peshawar 25000, PakistanMedicinal Botanic Centre, PCSIR Labs Complex, Peshawar 25120, PakistanMedicinal Botanic Centre, PCSIR Labs Complex, Peshawar 25120, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanHeliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000 mg/mL) and then several dilutions (50, 100, 150, 200, and 250 mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard. The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated to mass spectrometer was used. The essential fatty acids for growth maintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.http://dx.doi.org/10.1155/2014/829076 |
| spellingShingle | Sohail Ahmad Shabir Ahmad Ahtaram Bibi Muhammad Saqib Ishaq Muhammad Siddique Afridi Farina Kanwal Muhammad Zakir Farid Fatima Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum The Scientific World Journal |
| title | Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum |
| title_full | Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum |
| title_fullStr | Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum |
| title_full_unstemmed | Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum |
| title_short | Phytochemical Analysis, Antioxidant Activity, Fatty Acids Composition, and Functional Group Analysis of Heliotropium bacciferum |
| title_sort | phytochemical analysis antioxidant activity fatty acids composition and functional group analysis of heliotropium bacciferum |
| url | http://dx.doi.org/10.1155/2014/829076 |
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