Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation
Summary: The mitochondrial genome (mitogenome) is a vital tool in molecular phylogenetics, evolutionary biology, and population genetics. Here, we present a protocol for extracting mitochondrial DNA from shrimp (Macrobrachium rosenbergii) and prawn (Penaeus monodon) species using a differential cent...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002588 |
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| author | Dipta Chandra Pal Muhammad Manjurul Karim |
| author_facet | Dipta Chandra Pal Muhammad Manjurul Karim |
| author_sort | Dipta Chandra Pal |
| collection | DOAJ |
| description | Summary: The mitochondrial genome (mitogenome) is a vital tool in molecular phylogenetics, evolutionary biology, and population genetics. Here, we present a protocol for extracting mitochondrial DNA from shrimp (Macrobrachium rosenbergii) and prawn (Penaeus monodon) species using a differential centrifugation approach without relying on commercially available kits. We describe steps for sample preparation, crude mitochondrial extraction, mitochondrial DNA isolation, and purity assessment. We then detail procedures for confirming the presence of the mitogenome and sequencing the mitochondrial genome.For complete details on the use and execution of this protocol, please refer to Pal et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-47dc98c5ce6e4d91999158bdb22a23b3 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-47dc98c5ce6e4d91999158bdb22a23b32025-08-20T02:02:25ZengElsevierSTAR Protocols2666-16672025-06-016210385210.1016/j.xpro.2025.103852Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugationDipta Chandra Pal0Muhammad Manjurul Karim1Department of Microbiology, University of Dhaka, Dhaka 1000, BangladeshDepartment of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh; Corresponding authorSummary: The mitochondrial genome (mitogenome) is a vital tool in molecular phylogenetics, evolutionary biology, and population genetics. Here, we present a protocol for extracting mitochondrial DNA from shrimp (Macrobrachium rosenbergii) and prawn (Penaeus monodon) species using a differential centrifugation approach without relying on commercially available kits. We describe steps for sample preparation, crude mitochondrial extraction, mitochondrial DNA isolation, and purity assessment. We then detail procedures for confirming the presence of the mitogenome and sequencing the mitochondrial genome.For complete details on the use and execution of this protocol, please refer to Pal et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002588GenomicsMolecular BiologySequence analysisSequencing |
| spellingShingle | Dipta Chandra Pal Muhammad Manjurul Karim Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation STAR Protocols Genomics Molecular Biology Sequence analysis Sequencing |
| title | Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation |
| title_full | Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation |
| title_fullStr | Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation |
| title_full_unstemmed | Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation |
| title_short | Protocol for mitochondrial DNA isolation from Macrobrachium rosenbergii and Penaeus monodon using differential centrifugation |
| title_sort | protocol for mitochondrial dna isolation from macrobrachium rosenbergii and penaeus monodon using differential centrifugation |
| topic | Genomics Molecular Biology Sequence analysis Sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002588 |
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