Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms
The thermal unfolding of hemocyanin isoforms, β-HaH and αD+N-HaH, isolated from the hemolymph of garden snails Helix aspersa maxima, was studied by means of differential scanning calorimetry (DSC). One transition, with an apparent transition temperature (Tm) at 79.88°C, was detected in the thermogra...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8450792 |
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| author | Svetla Todinova Yuliana Raynova Krassimira Idakieva |
| author_facet | Svetla Todinova Yuliana Raynova Krassimira Idakieva |
| author_sort | Svetla Todinova |
| collection | DOAJ |
| description | The thermal unfolding of hemocyanin isoforms, β-HaH and αD+N-HaH, isolated from the hemolymph of garden snails Helix aspersa maxima, was studied by means of differential scanning calorimetry (DSC). One transition, with an apparent transition temperature (Tm) at 79.88°C, was detected in the thermogram of β-HaH in 20 mM HEPES buffer, containing 0.1 M NaCl, 5 mM CaCl2, and 5 mM MgCl2, pH 7.0, at scan rate of 1.0°C min−1. By means of successive annealing procedure, two individual transitions were identified in the thermogram of αD+N-HaH. Denaturation of both hemocyanins was found to be an irreversible process. The scan-rate dependence of the calorimetric profiles indicated that the thermal unfolding of investigated hemocyanins was kinetically controlled. The thermal denaturation of the isoforms β-HaH and αD+N-HaH was described by the two-state irreversible model, and parameters of the Arrhenius equation were calculated. |
| format | Article |
| id | doaj-art-47d318ac4d2642c69699c46eb069987e |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-47d318ac4d2642c69699c46eb069987e2025-02-03T06:08:12ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/84507928450792Calorimetric Study of Helix aspersa Maxima Hemocyanin IsoformsSvetla Todinova0Yuliana Raynova1Krassimira Idakieva2Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Bl. 21, Acad. G. Bonchev Str., Sofia 1113, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Bl. 9, Acad. G. Bonchev Str., Sofia 1113, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Bl. 9, Acad. G. Bonchev Str., Sofia 1113, BulgariaThe thermal unfolding of hemocyanin isoforms, β-HaH and αD+N-HaH, isolated from the hemolymph of garden snails Helix aspersa maxima, was studied by means of differential scanning calorimetry (DSC). One transition, with an apparent transition temperature (Tm) at 79.88°C, was detected in the thermogram of β-HaH in 20 mM HEPES buffer, containing 0.1 M NaCl, 5 mM CaCl2, and 5 mM MgCl2, pH 7.0, at scan rate of 1.0°C min−1. By means of successive annealing procedure, two individual transitions were identified in the thermogram of αD+N-HaH. Denaturation of both hemocyanins was found to be an irreversible process. The scan-rate dependence of the calorimetric profiles indicated that the thermal unfolding of investigated hemocyanins was kinetically controlled. The thermal denaturation of the isoforms β-HaH and αD+N-HaH was described by the two-state irreversible model, and parameters of the Arrhenius equation were calculated.http://dx.doi.org/10.1155/2018/8450792 |
| spellingShingle | Svetla Todinova Yuliana Raynova Krassimira Idakieva Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms Journal of Analytical Methods in Chemistry |
| title | Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms |
| title_full | Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms |
| title_fullStr | Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms |
| title_full_unstemmed | Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms |
| title_short | Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms |
| title_sort | calorimetric study of helix aspersa maxima hemocyanin isoforms |
| url | http://dx.doi.org/10.1155/2018/8450792 |
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