Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells
Previous studies report aberrant activation of the hedgehog signaling pathway in the progression of various cancers. This study aimed to investigate the expressions of smoothened and downstream glioma-associated oncogene homology-1 in gastric cancer and the underlying molecular mechanisms. Here, we...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317715546 |
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| _version_ | 1849253803175444480 |
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| author | Zixin Yang Yingqian Lv Long Wang Yong Chen Jinzhu Han Shan Zhao Wei Liu |
| author_facet | Zixin Yang Yingqian Lv Long Wang Yong Chen Jinzhu Han Shan Zhao Wei Liu |
| author_sort | Zixin Yang |
| collection | DOAJ |
| description | Previous studies report aberrant activation of the hedgehog signaling pathway in the progression of various cancers. This study aimed to investigate the expressions of smoothened and downstream glioma-associated oncogene homology-1 in gastric cancer and the underlying molecular mechanisms. Here, we first detected the expression in 58 cases of primary gastric cancer tissue and matched normal tissue specimens by western blot analysis and quantitative reverse transcription polymerase chain reaction. Cell proliferation and cycle were assayed in gastric cancer cells after blocking the hedgehog pathway by lentiviral-short hairpin RNA knockdown. In vitro inhibition of hedgehog pathway resulted in decreased cell proliferation and migration. Our studies demonstrate an important role for smoothened and glioma-associated oncogene homology-1 in gastric cancer and suggest inhibition of hedgehog pathway as a novel and potent strategy to treat gastric cancer patients. |
| format | Article |
| id | doaj-art-47c6fda1c9204de19c12cd1aab64dcb5 |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-47c6fda1c9204de19c12cd1aab64dcb52025-08-20T03:56:13ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317715546Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cellsZixin Yang0Yingqian Lv1Long Wang2Yong Chen3Jinzhu Han4Shan Zhao5Wei Liu6The Second Department of Medical Oncology, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaThe Second Department of Medical Oncology, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Medical Oncology, Northern Jiangsu People’s Hospital, Yangzhou, ChinaThe Second Department of Medical Oncology, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaThe Second Department of Medical Oncology, The Second Hospital of Hebei Medical University, Shijiazhuang, ChinaThe Palliative Medicine Center, Peking University Cancer Hospital, Peking, ChinaPrevious studies report aberrant activation of the hedgehog signaling pathway in the progression of various cancers. This study aimed to investigate the expressions of smoothened and downstream glioma-associated oncogene homology-1 in gastric cancer and the underlying molecular mechanisms. Here, we first detected the expression in 58 cases of primary gastric cancer tissue and matched normal tissue specimens by western blot analysis and quantitative reverse transcription polymerase chain reaction. Cell proliferation and cycle were assayed in gastric cancer cells after blocking the hedgehog pathway by lentiviral-short hairpin RNA knockdown. In vitro inhibition of hedgehog pathway resulted in decreased cell proliferation and migration. Our studies demonstrate an important role for smoothened and glioma-associated oncogene homology-1 in gastric cancer and suggest inhibition of hedgehog pathway as a novel and potent strategy to treat gastric cancer patients.https://doi.org/10.1177/1010428317715546 |
| spellingShingle | Zixin Yang Yingqian Lv Long Wang Yong Chen Jinzhu Han Shan Zhao Wei Liu Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells Tumor Biology |
| title | Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells |
| title_full | Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells |
| title_fullStr | Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells |
| title_full_unstemmed | Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells |
| title_short | Inhibition of hedgehog pathway reveals the regulatory role of SMO in gastric cancer cells |
| title_sort | inhibition of hedgehog pathway reveals the regulatory role of smo in gastric cancer cells |
| url | https://doi.org/10.1177/1010428317715546 |
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