Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets

The objective of this study was to investigate the effects of fermented wheat bran (FWB) on growth performance, nutrient digestibility, serum biochemistry, short-chain fatty acids, and intestinal microbiota of weaned piglets. One hundred twenty-eight weaned piglets were randomly assigned to 4 groups...

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Main Authors: Ninghui Jia, Jin Jin, Xinru Wei, Massimo Trabalza-Marinucci, Gang Jia, Qiang Zhou, Ruinan Zhang, Hua Li, Fali Wu, Hua Zhao, Hefeng Luo, Lianqiang Che, Jiayong Tang
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Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1561196/full
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author Ninghui Jia
Jin Jin
Xinru Wei
Massimo Trabalza-Marinucci
Gang Jia
Qiang Zhou
Ruinan Zhang
Hua Li
Fali Wu
Hua Zhao
Hefeng Luo
Lianqiang Che
Jiayong Tang
author_facet Ninghui Jia
Jin Jin
Xinru Wei
Massimo Trabalza-Marinucci
Gang Jia
Qiang Zhou
Ruinan Zhang
Hua Li
Fali Wu
Hua Zhao
Hefeng Luo
Lianqiang Che
Jiayong Tang
author_sort Ninghui Jia
collection DOAJ
description The objective of this study was to investigate the effects of fermented wheat bran (FWB) on growth performance, nutrient digestibility, serum biochemistry, short-chain fatty acids, and intestinal microbiota of weaned piglets. One hundred twenty-eight weaned piglets were randomly assigned to 4 groups, each with 8 pens and 4 piglets per pen: basal diet group (BD), 5% wheat bran group (5% WB), 5% fermented wheat bran group (5% FWB), and 10% fermented wheat bran group (10% FWB) for a 28-day trial. Results showed that compared to the BD group, the diarrhea rate in the 5% WB group was significantly increased (p < 0.05) at d 15–28 and d 1–28. In contrast, at d 15–28 and d 1–28, the diarrhea rates in the 5% FWB and 10% FWB groups were significantly lower than those in the 5% WB group and showed no significant difference compared to the BD group. Moreover, the apparent total tract digestibility (ATTD) of DM, GE, CP, EE, CF and ADF at d 1–14, and EE and NDF at d 15–28 in the 5% FWB group were significantly improved compared to the 5% WB group (p < 0.05). However, only the ATTD of CP, EE and CF at d 1–14 in the 10% FWB group were significantly higher than those in the 5% WB group (p < 0.01). Compared to the BD group, the pH of cecum chyme and serum urea nitrogen content in the 5% FWB and 10% FWB groups were significantly reduced (p < 0.05), and those in the 10% FWB group were significantly lower than those in the 5% WB group (p < 0.01). The propionic acid content of cecum chyme in the 5% FWB and 10% FWB groups, and butyric acid content in the 10% FWB group were significantly higher than those in the BD group (p < 0.05). LEfSe analysis (LDA score > 3.0) identified 4 species, 6 species of Proteobacteria, 2 species, and 9 species that were enriched in the BD, 5% WB, 5%F WB and 10%F WB groups, respectively. Additionally, Dialister, Prevotellaceae_NK3B31_group, Mitsuokella, Succinivibrio, and Prevotella were significantly and positively correlated with the concentrations of valeric acid, propionic acid, and acetic acid (p < 0.05). In conclusion, 10% FWB supplementation in weaned piglet diets did not affect growth performance, it reduced the diarrhea rate compared to the 5% WB group, potentially due to enhanced nutrient digestibility, elevated SCFAs levels, and shifts in microbial composition.
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spelling doaj-art-47c3f7e567204a77b7bfbccd1b513b132025-08-20T02:12:54ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.15611961561196Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned pigletsNinghui Jia0Jin Jin1Xinru Wei2Massimo Trabalza-Marinucci3Gang Jia4Qiang Zhou5Ruinan Zhang6Hua Li7Fali Wu8Hua Zhao9Hefeng Luo10Lianqiang Che11Jiayong Tang12Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaDepartment of Veterinary Medicine, University of Perugia, Perugia, ItalyKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaDekon Food and Agriculture Group, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaThe objective of this study was to investigate the effects of fermented wheat bran (FWB) on growth performance, nutrient digestibility, serum biochemistry, short-chain fatty acids, and intestinal microbiota of weaned piglets. One hundred twenty-eight weaned piglets were randomly assigned to 4 groups, each with 8 pens and 4 piglets per pen: basal diet group (BD), 5% wheat bran group (5% WB), 5% fermented wheat bran group (5% FWB), and 10% fermented wheat bran group (10% FWB) for a 28-day trial. Results showed that compared to the BD group, the diarrhea rate in the 5% WB group was significantly increased (p < 0.05) at d 15–28 and d 1–28. In contrast, at d 15–28 and d 1–28, the diarrhea rates in the 5% FWB and 10% FWB groups were significantly lower than those in the 5% WB group and showed no significant difference compared to the BD group. Moreover, the apparent total tract digestibility (ATTD) of DM, GE, CP, EE, CF and ADF at d 1–14, and EE and NDF at d 15–28 in the 5% FWB group were significantly improved compared to the 5% WB group (p < 0.05). However, only the ATTD of CP, EE and CF at d 1–14 in the 10% FWB group were significantly higher than those in the 5% WB group (p < 0.01). Compared to the BD group, the pH of cecum chyme and serum urea nitrogen content in the 5% FWB and 10% FWB groups were significantly reduced (p < 0.05), and those in the 10% FWB group were significantly lower than those in the 5% WB group (p < 0.01). The propionic acid content of cecum chyme in the 5% FWB and 10% FWB groups, and butyric acid content in the 10% FWB group were significantly higher than those in the BD group (p < 0.05). LEfSe analysis (LDA score > 3.0) identified 4 species, 6 species of Proteobacteria, 2 species, and 9 species that were enriched in the BD, 5% WB, 5%F WB and 10%F WB groups, respectively. Additionally, Dialister, Prevotellaceae_NK3B31_group, Mitsuokella, Succinivibrio, and Prevotella were significantly and positively correlated with the concentrations of valeric acid, propionic acid, and acetic acid (p < 0.05). In conclusion, 10% FWB supplementation in weaned piglet diets did not affect growth performance, it reduced the diarrhea rate compared to the 5% WB group, potentially due to enhanced nutrient digestibility, elevated SCFAs levels, and shifts in microbial composition.https://www.frontiersin.org/articles/10.3389/fvets.2025.1561196/fullfermented wheat brangrowth performanceintestinal healthnutrients digestibilityweaned piglet
spellingShingle Ninghui Jia
Jin Jin
Xinru Wei
Massimo Trabalza-Marinucci
Gang Jia
Qiang Zhou
Ruinan Zhang
Hua Li
Fali Wu
Hua Zhao
Hefeng Luo
Lianqiang Che
Jiayong Tang
Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
Frontiers in Veterinary Science
fermented wheat bran
growth performance
intestinal health
nutrients digestibility
weaned piglet
title Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
title_full Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
title_fullStr Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
title_full_unstemmed Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
title_short Effects of fermented wheat bran on growth performance, nutrient digestibility and intestinal microbiota of weaned piglets
title_sort effects of fermented wheat bran on growth performance nutrient digestibility and intestinal microbiota of weaned piglets
topic fermented wheat bran
growth performance
intestinal health
nutrients digestibility
weaned piglet
url https://www.frontiersin.org/articles/10.3389/fvets.2025.1561196/full
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